HIV-1 LTR
Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV 1 LTR
... Regulation of Cellular Genes by PAAF1 and/or Spt6 We next wished to determine whether PAAF1 and/or Spt6 regulate cellular genes in a similar fashion to the HIV-1 LTR. Thus, transcriptional profiling ...
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NUCKS1, a novel Tat coactivator, plays a crucial role in HIV 1 replication by increasing Tat mediated viral transcription on the HIV 1 LTR promoter
... the HIV-1 LAV strain, and ...Gibco-BRL), 1% L-glutamine, and 1% ...The HIV-1 molecu- lar clone pNL4-3 was obtained through the NIH AIDS Research and Reference Reagent Program, ...
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Selection of RNA aptamers that bind HIV 1 LTR DNA; strand invaders
... the LTR to define the binding sites. Various LTR-325 bp fragments containing 18 bp of the NdeI–XhoI–SalI linker sequences sequentially replacing the wild-type sequence from positions +1 to +325 were ...
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Apolipoprotein E isoform dependently affects Tat-mediated HIV-1 LTR transactivation
... Tat-mediated HIV-1 LTR transactivation using U87MG glioblastoma cells stably transfected with HIV-1 LTR-driven ...Tat-mediated HIV-1 LTR transactivation were ...
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Basal shuttle of NF κB/IκBα in resting T lymphocytes regulates HIV 1 LTR dependent expression
... the HIV-LTR, low but consistent transcrip- tional activity, which was enhanced by Tat expression, was detected ...level LTR activity in resting T cells, nuclear levels of IκBα were increased by LMB ...
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c-Myc and Sp1 Contribute to Proviral Latency by Recruiting Histone Deacetylase 1 to the Human Immunodeficiency Virus Type 1 Promoter
... of HIV-1 activation, a cotransfection of c-Myc and Tat expression vectors was performed along with the active and ...of HIV-1 LTR ...Tat-activated LTR RNA expression is ...
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Targeting Tat Inhibitors in the Assembly of Human Immunodeficiency Virus Type 1 Transcription Complexes
... an HIV-1 LTR-RREIIB-FFL reporter, and the values shown represent percent inhibition at a 1:1 activator/ inhibitor plasmid ratio relative to activation without ...and HIV-1 ...
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Inhibition of human immunodeficiency virus type 1 replication by a Tat-activated, transduced interferon gene: targeted expression to human immunodeficiency virus type 1-infected cells.
... type 1 (HIV-1) therapy in this ...transduced HIV-1 long terminal repeat (LTR)-IFNA2 (the new approved nomenclature for IFN genes is used throughout this article) hybrid gene to ...
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Original Article A high throughput RNAi screen reveals determinants of HIV-1 activity in host kinases
... a HIV-1 LTR-driven reporter ...As HIV-1 LTR contains NFκB motifs, numerous studies suggest that host NFκB signaling plays a critical role in acti- vation of HIV-1 ...
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Role of tumor necrosis factor alpha in activation and replication of the tat-defective human immunodeficiency virus type 1.
... Whereas in acute HIV-1 infection, Tat is the major transcriptional transactivator, the activation of the HIV-1 LTR and the latent endogenous HIV-1 provirus by different extracellular sti[r] ...
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Differential effects of human cytomegalovirus on integrated and unintegrated human immunodeficiency virus sequences.
... on HIV-1 ...integrated HIV-1 LTR, we devised a simple technique for obtaining stably transfected cell lines with a single, but random, site of ...an LTR-chloramphenicol ...
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RNA 3′ Readthrough of Oncoretrovirus and Lentivirus: Implications for Vector Safety and Efficacy
... 3⬘ LTR in an MLV construct to stabilize the readthrough RNA and to allow internal translation of nlCre ...and HIV-1 poly(A) signals, we recloned the wt LTR and the SIN LTR of both MLV ...
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The interferon induced antiviral protein PML (TRIM19) promotes the restriction and transcriptional silencing of lentiviruses in a context specific, isoform specific fashion
... observed HIV-1 restriction phenotype in MEFs in two ...with HIV-1 infection and/or tran- scription by upregulating the production of type I ...subunit 1 (IFNAR- 1) ...of ...
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Expression of a human immunodeficiency virus type 1 long terminal repeat/simian virus 40 early region fusion gene in transgenic mice.
... We investigated the in vivo tissue specificity of the HIV-1 transcription control elements by introducing a hybrid reporter gene containing the HIV-1 LTR fused to the simian virus 40 SV4[r] ...
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Epstein-Barr virus nuclear antigen 2 transactivates the long terminal repeat of human immunodeficiency virus type 1.
... To identify the cis-regulatory sequences of the HIV-1 LTR target for EBNA2 activation, we performed cell transfection experiments in which the wild-type or mutant HIV-1 LTR plasmids carr[r] ...
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Utilization of HIV 1 envelope V3 to identify X4 and R5 specific Tat and LTR sequence signatures
... The four Tat amino acid positions of interest are located within domains responsible for transactivation, cysteine-rich, TAR binding, and nuclear localization, respectively (Fig. 5). In order to further evaluate the ...
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Tumor Suppressor Cylindromatosis (CYLD) Controls HIV Transcription in an NF-κB-Dependent Manner
... modulates HIV infection by limiting vi- ral transcription through negative regulation of the NF- B path- ...that HIV-1 LTR-driven transcription is increased in the absence of CYLD in an ...
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Transient Induction of Cyclin T1 during Human Macrophage Differentiation Regulates Human Immunodeficiency Virus Type 1 Tat Transactivation Function
... LPS reinduces CycT1 expression at late time points in MDMs. Circulating monocytes migrate to tissues and undergo differentiation to become resident macrophages. These tissue macrophages can be activated by pathogens or ...
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The tumor suppressor protein p53 strongly alters human immunodeficiency virus type 1 replication.
... To examine the role of wt-p53 in HIV-1 LTR-directed transcriptional regulation, full-length HIV-1 LTR-CAT plasmids were cotransfected with different quantities of the p53 expression vect[r] ...
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Mutational analysis of human T-cell leukemia virus type I Tax: regions necessary for function determined with 47 mutant proteins.
... By assaying each mutant protein separately on the HTLV-I long terminal repeat LTR and the human immunodeficiency virus type 1 HIV-1 LTR in parallel, we were able to identify regions of T[r] ...
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