HIV-1 viral load estimation using Artus assay:
Evaluation of Effect of Specimen Handling Parameters for Plasma Preparation Tubes on Viral Load Measurements Obtained by Using the Abbott RealTime HIV 1 Load Assay
... Abbott HIV-1 RealTime assay uses automated extrac- tion of viral nucleic acid, which is performed on the m2000sp using purification reagents specific for RNA, followed by am- ...
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Assessment of the Cavidi ExaVir Load Assay for Monitoring Plasma Viral Load in HIV 2 Infected Patients
... ABSTRACT HIV plasma viral load is an established marker of disease progression and of response to antiretroviral therapy, but currently there is no commercial assay validated for the ...
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Comparative evaluation of the performance of the Abbott RealTime HIV 1 assay for measurement of HIV 1 plasma viral load on genetically diverse samples from Greece
... for HIV-1 viral load measure- ments offer the potential of increased sensitivity, expanded dynamic range, diminished risk of contamina- tion and high ...accurate viral load ...
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Role of HIV 1 Viral Load in Initiating Antiretroviral Therapy
... TaqMan® HIV-1 test. 2.1. Estimation of HIV Viral Load Principles of the Procedure The COBAS® TaqMan® HIV-1 Test is based on three major processes: 1) manual ...
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The mouse viral outgrowth assay: avatars for the detection of HIV 1 reservoirs
... cell-associated HIV-1 RNA ...no HIV-1 DNA, RNA or replication-competent virus was detected from PBMCs or cells isolated from the ileum, rectum, lymph nodes, bone marrow and cerebrospinal fluid ...
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GeneXpert HIV 1 quant assay, a new tool for scale up of viral load monitoring in the success of ART programme in India
... GeneXpert assay for all PLHIV with presumptive TB to ensure early diagnosis of TB will help on convergence of load per day for TB where less number of machines is available at district ...one assay ...
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A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting
... commercial HIV-1 viral load assays are designed and optimized for the detection of viruses found in North America and ...the HIV-1 non-B subtypes ...C HIV-1 viruses ...
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Comparative Performances of HIV 1 RNA Load Assays at Low Viral Load Levels: Results of an International Collaboration
... reported using EDTA tubes for sample collection and ...version 1 assay is being phased out or is no longer in use, and the Amplicor assay has been ...Amplicor assay are of limited ...
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Quantitative Analysis of Human Herpesvirus 8 Viral Load Using a Real Time PCR Assay
... over- estimation of the sensitivity of the ...PCR assay or by conventional PCR assays (data not ...to 1 copies per well, and each dilution was submitted to four independent ...Table 1, the ...
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Leukodepletion as a Point of Care Method for Monitoring HIV 1 Viral Load in Whole Blood
... for HIV-1 RNA quantification. For HIV-1 RNA quantifi- cation, in-house reverse transcription (RT)-qPCR was used, as previously described ...semiquantitative assay. The Samba ...
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Evaluation of the Whole Blood Alere Q NAT Point of Care RNA Assay for HIV 1 Viral Load Monitoring in a Primary Health Care Setting in Mozambique
... recommend using viral load test data as a binary result above or below a specific virological failure threshold, determining ART success may be more appro- priate using a trend analysis, as is ...
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Lower Sensitivity and Avidity Modifications of the Vitros Anti HIV 1+2 Assay for Detection of Recent HIV Infections and Incidence Estimation
... 5). Using additional assays, such as the BED assay, further improves the overall FRR to 5% (95% CI, ...of HIV infection dynamics in a population, one needs to calculate the incidence of infection in ...
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Evaluation of Quantification of HIV 1 RNA Viral Load in Plasma and Dried Blood Spots by Use of the Semiautomated Cobas Amplicor Assay and the Fully Automated Cobas Ampliprep/TaqMan Assay, Version 2 0, in Kisumu, Kenya
... CAP/CTM using DBS speci- mens may be appropriate under the WHO guidelines for detecting viral loads above the threshold of 5,000 copies/ml, further study using specimens from HIV-infected ...
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Performance of the Xpert HIV 1 Viral Load Assay: a Systematic Review and Meta analysis
... Xpert HIV-1 VL and the reference test were used to classify patients above or below certain ...Xpert HIV-1 VL would allow for direct comparisons in systematic ...predominant HIV subtype ...
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Evaluation of the RealTime HIV 1, Xpert HIV 1, and Aptima HIV 1 Quant Dx Assays in Comparison to the NucliSens EasyQ HIV 1 v2 0 Assay for Quantification of HIV 1 Viral Load
... DISCUSSION HIV-1 viral loads in the blood determine the risk of disease pro- gression and guide antiretroviral treatment decisions ...clinical HIV laboratories, accurate sensitive viral ...
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Use of Sno Strip Filter Paper Wicks for Collection of Genital Tract Samples Allows Reproducible Determination of Human Immunodeficiency Virus Type 1 (HIV 1) RNA Viral Load with a Commercial HIV 1 Viral Load Assay
... of HIV-1 RNA have included Dacron swabs, cervicovaginal lavage, and absorption wicks (1, 12, ...genital viral load measure- ments, it is important to ensure that such measurements are ...
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IMPLEMENTING HIV VIRAL LOAD TESTING
... from viral load requisition forms can provide key information, such as the pace of clinical and laboratory roll-out, types of people receiving viral load testing, viral suppression ...
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Point of Care HIV Viral Load Testing:
... Staff: Lab specialists, senior techs, Programme officer Dx: HIV serology by ELISA, other ELISA, CD4 count, basic. chemistry, haematology & microbiology[r] ...
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Cumulative Impact of Host and Viral Factors on HIV-1 Viral-Load Control during Early Infection
... overall viral fitness ...and viral determinants of control of viremia in recently infected individuals, and it may explain a portion of the observed variabil- ity in responses to similar antiretroviral drug ...
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Viral genetic variation accounts for a third of variability in HIV-1 set-point viral load in Europe
... on viral load can be described as a linear model with a fixed effect representing the expectation of viral load at the tips and a ran- dom effect with a covariance structure depending on the ...
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