HMC-1
Brennfleck, Frank Werner (2018): Etablierung eines Tetrazyklin-induzierbaren Genexpressions-Systems zur Untersuchung des Ras-Guaninnukleotid-Austauschfaktors 4 in der humanen Mastzelllinie HMC-1. Dissertation, LMU München: Medizinische Fakultät
... Mastzelllinie HMC-1 wurde 1988 von Butterfield et ...exprimieren HMC-1-Zellen keinen IgE-Rezeptor, nur geringe Mengen der Tryptase und keine Chymase ...LAD 1 und LAD 2 exprimieren neben ...
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Geraniol suppresses proinflammatory mediators in phorbol 12-myristate 13-acetate with A23187-induced HMC-1 cells
... C. HMC-1 cells were exposed to geraniol for 1 hour before treatment with PMACI (20 nM of PMA plus 1 μ M of A23187) and incubated at 37 ° ...
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N822K- or V560G-mutated KIT activation preferentially occurs in lipid rafts of the Golgi apparatus in leukemia cells
... and HMC-1.1. As shown in Additional file 1: Figure S3C, KIT signals remained in BafA1-treated cells, indi- cating that endosome-to-lysosome trafficking is un- necessary for downstream ...file 1: ...
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A novel heparin dependent processing pathway for human tryptase Autocatalysis followed by activation with dipeptidyl peptidase I
... Fig. 1, pro- teolytic processing of rhb-protryptase by an HMC-1 extract occurred in the absence of exogenous heparin or dextran sul- fate, but without tryptase enzymatic ...
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Infiltrating mast cells enhance prostate cancer invasion via altering LncRNA-HOTAIR/PRC2-androgen receptor (AR)-MMP9 signals and increased stem/progenitor cell population
... treated HMC-1 cells with the differentiation reagent phorbol 12-myristate 13-acetate (PMA) to induce the mast cell differentiation and ...cells HMC-1 co-cultured with 4 different PCa cells ...
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Adenosine A2b receptors evoke interleukin 8 secretion in human mast cells An enprofylline sensitive mechanism with implications for asthma
... mechanisms involved have not been defined. We, therefore, investigated the effects of adenosine on the human mast cell line HMC-1. Both the A2a selective agonist CGS21680 and the A2a/A2b nonselective ...
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Dengue Virus Selectively Induces Human Mast Cell Chemokine Production
... Severe dengue virus infections usually occur in individuals who have preexisting anti-dengue virus antibod- ies. Mast cells are known to play an important role in host defense against several pathogens, but their role in ...
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Effects of Ixeris dentata water extract and caffeic acid on allergic inflammation in vivo and in vitro
... The HMC-1 cell line was established from leukemia patients; it expresses characteristic markers for mast cells ...A23187-stimulated HMC-1 cells ...
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Enhanced effects of cigarette smoke extract on inflammatory cytokine expression in IL-1β-activated human mast cells were inhibited by Baicalein via regulation of the NF-κB pathway
... Previously, glucocorticoids that have frequently been used for the treatment of inflammatory diseases, allergy, and autoimmune diseases were thought to suppress NF- B activation. Glucocorticoids induce the transcription ...
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Mast cells dysregulate apoptotic and cell cycle genes in mucosal squamous cell carcinoma
... HMC-1 down-regulates CDK6 expression in SCC25. CDKs are important regulators of cell cycle progression. CDK6, which first appears in mid-G1 phase, is important for G1 phase progression and G1/S transition. ...
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Evidence for a novel Kit adhesion domain mediating human mast cell adhesion to structural airway cells
... to HMC-1 cells that had been epitope-masked as described by Popkov et ...the HMC-1 cells (1-2 × 10 7 cells) were incubated with 200 μl of a pool of the anti-mast cell serum R91 and R92 ...
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An improved 3D tetraculture system mimicking the cellular organisation at the alveolar barrier to study the potential toxic effects of particles on the lung
... Furthermore the ratio of A549, THP-1 and HMC-1 cells was optimized in order to reproduce a physiologic- ally relevant cell density and distribution [29]. In the alveolar model presented in this ...
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Human mast cells decrease SLPI levels in type II – like alveolar cell model, in vitro
... The role of mast cells as primary effector cells in IgE- dependent, immediate hypersensitivity is well established [57]. The discovery that mast cells can release a wide variety of immune mediators, including proteases, ...
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Dengue Virus Infection of Mast Cells Triggers Endothelial Cell Activation
... line HMC-1 (7) were mock treated using conditioned medium from uninfected Vero cells or ex- posed (multiplicity of infection [MOI] of 1 to 3) to dengue virus alone or dengue virus or UV-inactivated ...
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Baicalein inhibits IL-1β- and TNF-α-induced inflammatory cytokine production from human mast cells via regulation of the NF-κB pathway
... NB in the nucleus [42]. Additionally, 12-lipoxygenase (12- LOX) has been implicated as a mediator of inflammation, atherosclerosis, and cancer [43-45]. Several in vitro studies have suggested 12/15-LOX products to be ...
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Andrographolide suppresses thymic stromal lymphopoietin in phorbol myristate acetate/calcium ionophore A23187-activated mast cells and 2,4-dinitrofluorobenzene-induced atopic dermatitis-like mice model
... HMC-1 cells were grown on glass chamber slides for 24 hours and pretreated with ANDRO (50 μ M) for 2 hours, followed by PMACI stimulation for 20 minutes. Cells were then fixed with 4% paraformaldehyde in ...
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ALDH/CD44 identifies uniquely tumorigenic cancer stem cells in salivary gland mucoepidermoid carcinomas
... Human salivary gland mucoepidermoid carcinoma cell lines (UM-HMC-1, UM-HMC-3A, UM-HMC-3B) previously characterized in our laboratory [38] were cultured in high glucose Dulbecco’s Modified ...
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Signal pathways in astrocytes activated by cross-talk between of astrocytes and mast cells through CD40-CD40L
... score 1, loss of weight and tail weakness; score 2, weakness in hind limb; score 3, complete hind limb paralysis; score 4, hind limb paralysis with forelimb weakness or paralysis; and score 5, moribund or ...
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Transfer of microRNAs by extracellular membrane microvesicles: a nascent crosstalk model in tumor pathogenesis, especially tumor cell-microenvironment interactions
... Table 1, in view of this field, the plasma EMV-containing miRNA panel as a sensitive and tumor-specific marker has become a hot topic in the majority of solid tumors, to a large extent, which represents the ...
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SURVEY ON LOAD BALANCING IN MOBILE CLOUD COMPUTING
... C.Chen et. al., [10] proposed a Heterogeneous Mobile Cloud (HMC) computing design that efficiently utilizes the communication and computation resources to support data storage and data processing services in a ...
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