Holliday junction
Holliday junction processing enzymes in eukaryotes
... All Holliday junction-specific endonucleases studied so far have been shown to bind and cleave the junction as dimers (reviewed in (White et ...the Holliday junction would be consistent ...
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Fission Yeast Mus81·Eme1 Holliday Junction Resolvase Is Required for Meiotic Crossing Over but Not for Gene Conversion
... D URING meiosis, homologous recombination serves within a gene, appropriate correction of base mis- at least two purposes—to increase genetic diver- matches would give rise to one but not the reciprocal sity, upon which ...
6
Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry
... in Holliday junction recognition and sequence specificity of resolution (Hagan et ...than Holliday junctions, like similar phage resolvases but unlike ...4-stranded Holliday junc- tion with ...
19
Repression of Vaccinia Virus Holliday Junction Resolvase Inhibits Processing of Viral DNA into Unit-Length Genomes
... resembling a four-way Holliday junction (HJ) recombination intermediate (10, 28). Extracts of poxvirus-infected cells con- tain an HJ resolving activity (43). In addition, poxvirus-en- coded topoisomerase ...
12
Holliday junction resolvase in Schizosaccharomyces pombe has identical endonuclease activity to the CCE1 homologue YDC2
... The specificity of the resolvase activity was further confirmed by testing duplex and single-stranded DNAs as competitors. The activity was not affected by the presence of a 1000-fold molar excess of linear duplex ...
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The RuvAB Branch Migration Translocase and RecU Holliday Junction Resolvase Are Required for Double-Stranded DNA Break Repair in Bacillus subtilis
... In models of Escherichia coli recombination and DNA repair, the RuvABC complex directs the branch migration and resolution of Holliday junction DNA. To probe the validity of the E. coli paradigm, we ...
11
The Mus81/Mms4 Endonuclease Acts Independently of Double-Holliday Junction Resolution to Promote a Distinct Subset of Crossovers During Meiosis in Budding Yeast
... Current models for meiotic recombination require that crossovers derive from the resolution of a double-Holliday junction (dHJ) intermediate. In prokaryotes, enzymes responsible for HJ resolution are well ...
14
Lee, Shun-Hsiao (2017): Structural and biochemical characterization of human GEN1 holliday junction resolvase. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... strict Holliday junction resolvase that only recognizes four-way junction and is not able to cut 5’ flap and replication fork DNA, supporting the hypothesis that the human GEN1 α6 plays a critical ...
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Vraschek, Carina Christine (2018): Structural and biochemical studies on holliday junction recognition by the SLX4-MUS81 complex. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... SLX4 interacts as a platform protein with other proteins involved in DNA repair, especially structure-specific endonucleases like MUS81-EME1 and SLX1 [113–115]. The human 200 kDa SLX4 protein consists of six domains ...
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RusA Holliday junction resolvase: DNA complex structure - insights into selectivity and specificity
... We have determined the structure of a catalytically inactive D70N variant of the Escherichia coli RusA resolvase bound to a duplex DNA substrate that reveals critical protein–DNA interactions and per- mits a much clearer ...
8
Molecular mechanism of double Holliday junction dissolution
... double-Holliday junction dissolution reaction and directly interacts with both the BLM helicase and TopoIIIα ...of Holliday junction migration, as shown in yeast, it has an important role ...
5
Characterisation of a Holliday junction resolving enzyme from Schizosaccharomyces pombe
... Although junction-resolving activities have been detected in ...four-way junction-resolving ...a Holliday junction ...liday junction resolution between these two organisms suggests that ...
7
In vitro resolution of poxvirus replicative intermediates into linear minichromosomes with hairpin termini by a virally induced Holliday junction endonuclease.
... 3 0022-538X/92/031551-13$02.00/0 Copyright C 1992, American Society for Microbiology In Vitro Resolution of Poxvirus Replicative Intermediates into Linear Minichromosomes with Hairpin Te[r] ...
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Characterisation of potential Holliday junction resolvases in Trypanosoma brucei
... Trypanosoma are a genus of disease causing protozoan parasite. Trypanosoma brucei (T. brucei), which is the focus of this work, is the cause of two diseases, Human African Trypanosomiasis (HAT) and Nagana. The disease ...
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The Holliday junction on its thirtieth anniversary.
... LEY RADDING (1975) altered ROBIN’S model to give it the flexibility required to handle data both from yeast and from fungi that did show appreciable reciprocality in heter[r] ...
6
Opposing Roles of the Holliday Junction Processing Systems of Escherichia coli in Recombination-Dependent Adaptive Mutation
... Temporary absence of both the RuvABC and RecG resolution systems promotes Lac+ adaptive hypermuta- tion: Conjugational recombination is blocked by the ab- sence of RuvA[r] ...
11
Holliday junction–recognizing protein promotes cell proliferation and correlates with unfavorable clinical outcome of hepatocellular carcinoma
... We speculated that ectopic HJURP expression may promote the progression of HCC cell lines; therefore, we detected mRNA level in HCC tissues and corresponding normal live[r] ...
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The Crystal Structure of D212 from Sulfolobus Spindle-Shaped Virus Ragged Hills Reveals a New Member of the PD-(D/E)XK Nuclease Superfamily
... archaeal Holliday junction cleavage enzymes like Hjc and Hje (8, 34, 37); TnsA, which is a component of TN7 transposase (19); and the homing endonuclease I-SspI ...
8
Analysis of One-Sided Marker Segregation Patterns Resulting From Mammalian Gene Targeting
... double Holliday junction ...the Holliday junction to the left and right of the DSB generates nicks at positions 2,29 and 4,49, respectively, which direct repair of adjacent mismatches in hDNA ...
15
Different genome stability proteins underpin primed and naïve adaptation in E coli CRISPR Cas immunity
... a Holliday junction structure (43,67), was ini- tially appealing to us for facilitating primed adaptation, by Cas1 cleaving a Holliday junction to generate ends for DNA ...represent ...
10