HPV16 E7
Realgar, a traditional Chinese medicine, induces apoptosis of HPV16-positive cervical cells through a HPV16 E7-related pathway
... and HPV16 E7, we detected the protein expression of these proteins by Western ...the HPV16 E7 and Bax expressions were markedly ...of HPV16 E6 was constant in both the ...
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CUL2 overexpression driven by CUL2/E2F1/miR-424 regulatory loop promotes HPV16 E7 induced cervical carcinogenesis
... that HPV16 E7, but not other genotypes, can bind to scaffold protein CUL2 during inducing cervical carcinogenesis, but the expression level, associated regulating mechanism, and potential carcinogenicity of ...
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Combination of proteasome and HDAC inhibitor enhances HPV16 E7-specific CD8+ T cell immune response and antitumor effects in a preclinical cervical cancer model
... with HPV16 E7 peptide ...and E7-specificity using E7 peptide- loaded H-2D b ...of E7-specific CD8+ T cells among PBMCs compared to those treated with bortezomib or SAHA alone (Figure 2C ...
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Hypoxia inducible factor-1 alpha expression is increased in infected positive HPV16 DNA oral squamous cell carcinoma and positively associated with HPV16 E7 oncoprotein
... of HPV16 E7 oncoprotein It was analyzed whether high-risk HPV16 E7 oncopro- tein were detectable by immunohistochemical ...No HPV16 E7 oncoprotein staining could be detected in ...
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Enhancing antitumor immunogenicity of HPV16-E7 DNA vaccine by fusing DNA encoding E7-antigenic peptide to DNA encoding capsid protein L1 of Bovine papillomavirus
... The papillomavirus (PV) major capsid protein has been shown to spontaneously self-assemble into virus-like particles (VLPs) upon administration and expression [8, 9]. Additionally, VLPs are non-infectious, as they do not ...
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<p>The Intracellular Delivery Of Anti-HPV16 E7 scFvs Through Engineered Extracellular Vesicles Inhibits The Proliferation Of HPV-Infected Cells</p>
... Purpose: Single-chain variable fragments (scFvs) are one of the smallest antigen-binding units having the invaluable advantage to be expressed by a unique short open reading frame (ORF). Despite their reduced size, ...
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Recombinant HPV16 E7 assembled into particles induces an immune response and specific tumour protection administered without adjuvant in an animal model
... of HPV16 is mainly ascribed to the viral oncoprotein E7, which has been shown to interact with a variety of cellular ...proteins. HPV16 E7 is a 98-amino-acid phos- phoprotein (11 kDa) that ...
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Original Article Therapeutic and anti-metastatic effect of CIKs on cervical cancer enhanced by modified HPV16 E7-pulsed DCs with SOCS1-knockdown
... modified HPV16 E7 (HPV16mE7) with reduced transforma- tion activity and enhanced antigenicity was employed to load DCs with silenced suppressor of cytokine signaling 1 (SOCS1) ...of HPV16 ...
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Amino-functionalized poly(L-lactide) lamellar single crystals as a valuable substrate for delivery of HPV16-E7 tumor antigen in vaccine development
... histidine-tagged E7 protein were lysed in a denaturing buffer containing 8 M urea, 10 mM NaH 2 PO 4 , 10 mM Tris- HCl (pH 8), 300 mM NaCl, 1 mM dithiothreitol, 1% Triton X-114, and 1% Triton X-100 (Buffer B ...the ...
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<p>Self-Assembled Nanofibers Elicit Potent HPV16 E7-Specific Cellular Immunity And Abolish Established TC-1 Graft Tumor</p>
... of E7-speci fi c IFN- γ -expressing splenocytes detected by ELISPOT and the higher frequency of key effector IFN- γ + CD4 + Th1 cells and IFN- γ + CD8 + CTL in isolated splenocytes in the vaccinated ...of E7 ...
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A novel HPV16 E7-affitoxin for targeted therapy of HPV16-induced human cervical cancer
... HTB-35, HPV16 positive, contains about one to two copies of integrated HPV16 genome), CaSki (ATCC: CRL-1550, HPV16 positive, contains about 600 copies of integrated HPV16 genome), HeLa 229 ...
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Carboplatin/paclitaxel, E7-vaccination and intravaginal CpG as tri-therapy towards efficient regression of genital HPV16 tumors
... orthotopic HPV16 TC-1 model, herein we sequentially investigated in progressively more stringent settings the effects of systemic administration of carboplatin/paclitaxel (C + P) chemotherapy combined with ...
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High-Risk Alphapapillomavirus Oncogenes Impair the Homologous Recombination Pathway
... expressed HPV16 E6 and E7 (HPV E6 and E7) together and separately in primary human keratinocytes ...of HPV16 E6 and E7, p53 and pRB, respectively, as well as by qRT-PCR of 16 HPV E6 and ...
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Serine/threonine kinases 31(STK31) may be a novel cellular target gene for the HPV16 oncogene E7 with potential as a DNA hypomethylation biomarker in cervical cancer
... and E7 mediate the onco- genic activities of hrHPVs and are crucial for hrHPV- induced malignant cell transformation; furthermore these oncoproteins have been proven to affect human CC through different pathways ...
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Interactions with Pocket Proteins Contribute to the Role of Human Papillomavirus Type 16 E7 in the Papillomavirus Life Cycle
... That E7 can inactivate pRb function without degrading pRb, as evidenced by the efficient induction of MCM7 in rafts of cells harboring wild-type or E7 ⌬PTLHE mutant HPV16 repli- cons, as well as the ...
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Proteomics-based identification of VDAC1 as a tumor promoter in cervical carcinoma
... The HPV16 E7 expression plasmid was a gift from Harald zur Hausen (German Cancer Research Center, University of Heidelberg, Heidelberg, Germany) and the specific overexpression plasmid targeting VDAC1 was ...
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Papillomavirus E7 Oncoproteins Share Functions with Polyomavirus Small T Antigens
... HPV16 E7 blocks myoblast differentiation. We next tested the effects of HPV16 oncoproteins on myoblast differentiation in the C2C12 ...of HPV16 oncoproteins in the same ...ST, HPV16 E6, ...
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The human papillomavirus type 16 E7 protein complements adenovirus type 5 E1A amino-terminus-dependent transactivation of adenovirus type 5 early genes and increases ATF and Oct-1 DNA binding activity.
... HPV E7 protein indeed comple- ments the deficiencies of the large-deletion mutant E1A pro- teins, cells known to express HPV E7 were analyzed for per- missiveness for Ad5 E1A mutant ...the HPV16 ...
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Original Article Regulator role of HPV E7 protein on miR-21 expression in cervical carcinoma cells and its functional implication
... summary, HPV16 E7 pro- tein can stimulate the expression of miR-21 in host cells, thus facilitating cervical cancer cell growth, proliferation and ...of E7 in pathogene- sis and progression of ...
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Small Tumor Virus Genomes Are Integrated near Nuclear Matrix Attachment Regions in Transformed Cells
... in HPV16 E7 protein levels has been noted among subclonal populations of cervical epithelial cells harboring exclusively integrated HPV16 DNA ...cellular E7 protein levels was demonstrated and ...
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