HRP-2
Performance of four HRP 2/pLDH combination rapid diagnostic tests and field microscopy as screening tests for malaria in pregnancy in Indonesia: a cross sectional study
... Results: Overall 950 were recruited and 98.7 % were asymptomatic. The prevalence of malaria was 3.0–3.4 % by RDTs, and 3.6, 5.0 and 6.6 % by field microscopy, expert microscopy and PCR, respectively. The geometric-mean ...
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Performance of HRP 2 based rapid diagnostic test for malaria and its variation with age in an area of intense malaria transmission in southern tanzania
... performed. HRP-2 test sensitivity, specificity, positive and negative predictive values were calculated and compared between distinct age groups, using microscopy as “gold ...the HRP-2 test. ...
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HRP 2 determines HIV 1 integration site selection in LEDGF/p75 depleted cells
... file 2 and 3), suggesting only subtle differences between LEDGF/p75 and HRP-2 for the interaction with the local ...and HRP-2 double KD shifted integration significantly out of ...
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Lessons learned from the use of HRP 2 based rapid diagnostic test in community wide screening and treatment of asymptomatic carriers of Plasmodium falciparum in Burkina Faso
... A possible explanation is that several subjects may have harboured parasites at low densities that were below de- tection limit of the HRP-2 test. The proportion of subjects with parasite count <500 ...
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Direct comparison of the histidine rich protein 2 enzyme linked immunosorbent assay (HRP 2 ELISA) and malaria SYBR green I fluorescence (MSF) drug sensitivity tests in Plasmodium falciparum reference clones and fresh ex vivo field isolates from Cambodia
... Dried drug plates, for use in the HRP-2 and MSF assays, were prepared using published methods [9]. Briefly, six drugs, dihydroartemisinin (DHA), artesunate (AS), meflo- quine hydrochloride (MQ), quinine ...
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Optimizing the HRP 2 in vitro malaria drug susceptibility assay using a reference clone to improve comparisons of Plasmodium falciparum field isolates
... The value of using an artemisinin-susceptible W2 clone to control for the inherent variability in the HRP-2 in vitro assay was demonstrated here, particularly with field iso- lates, defining a range for ...
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Performance of a HRP 2/pLDH based rapid diagnostic test at the Bangladesh India Myanmar border areas for diagnosis of clinical malaria
... Several evaluation studies of Parascreen in different countries reported overall sensitivity ranging from 47.5 to 95.5% and specificity from 64.3 to 98.5% with varying performance for falciparum and non-falciparum ...
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A CRITICAL REVIEW OF DESIGN AND TYPES OF HUMANOID ROBOT
... The mechanical design of the humanoid requires careful and complex tradeoffs between form, function, power, weight, cost and manufacturability. For example, in terms of form, the robot should conform to the proportions ...
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Field evaluation of a PfHRP 2/pLDH rapid diagnostic test and light microscopy for diagnosis and screening of falciparum malaria during the peak seasonal transmission in an endemic area in Yemen
... is still the gold standard for species identification and detecting the severity of malaria by quantifying parasitae- mia and for differentiation of transmissible stages from those responsible for clinical disease [26]. ...
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Detection of the Plasmodium falciparumAntigen Histidine Rich Protein 2 in Blood of Pregnant Women: Implications for Diagnosing Placental Malaria
... Table 2 also reveal that the ELISA detected HRP-2 in 49% of the samples obtained from preg- nant women who were diagnosed as being malarial parasite negative; ...that HRP-2 may persist ...
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Evaluation of the Palutop+4 malaria rapid diagnostic test in a non endemic setting
... the HRP-2 line and its co-presence with the pan-pLDH line were indicative for the parasite density, this prediction was not as strict and conclusive as observed in other malaria RDTs ...
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Application of loop mediated isothermal amplification for malaria diagnosis during a follow up study in São Tomé
... The diagnostic testing effort in STP is high. The an- nual blood examination rate exceeds 30% on average, far greater than in other countries in the region [2]. How- ever, current surveillance systems rely on ...
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Placental Plasmodium falciparum malaria infection: Operational accuracy of HRP2 rapid diagnostic tests in a malaria endemic setting
... However, malaria rapid diagnostic tests (RDTs) may provide a solution as emerging evidence suggests that RDTs are capable of detecting placental malaria better than microscopy and may detect sub-microscopic infec- tions ...
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Attenuation of Plasmodium falciparum in vitro drug resistance phenotype following culture adaptation compared to fresh clinical isolates in Cambodia
... Results: IC 50 for culture-adapted specimens were significantly lower compared to the original fresh isolates for both HRP-2 and SYBR-Green I assays, with greater than a 50 % decline for the majority of ...
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Prozone in malaria rapid diagnostics tests: how many cases are missed?
... Some limitations are however to be mentioned. The wet season of 2009-2010 in Mozambique was character- ized by irregular rainfall and long dry spells. Due to this low rainfall (30% below expected value in November 2009 ...
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Performance comparison of CareStart™ HRP2/pLDH combo rapid malaria test with light microscopy in north western Tigray, Ethiopia: a cross sectional study
... protein 2 (HRP-2), plasmodium specific lactate dehydrogenase (pLDH) and aldolase antigens [12, ...detects HRP- 2 and PAN-pLDH had shown good results for the diagnosis of different ...
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Test characteristics of two rapid antigen detection tests (SD FK50 and SD FK60) for the diagnosis of malaria in returned travellers
... the HRP-2 antigen ...(> 2) years compared to those stored for a shorter period (results not shown), and the samples had not been exposed to repeat thawing and ...
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The gradient of Gurken, a long range morphogen, is directly regulated by Cbl mediated endocytosis
... punctate HRP signals were dramatically increased in follicle cells with medium overexpression of CblL, but were not detectable in cells with high expression levels of CblL ...
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Paper Microzone Plates as Analytical Tools for Studying Enzyme Stability: A Case Study on the Stabilization of Horseradish Peroxidase Using Trehalose and SU-8 Epoxy Novolac Resin
... the HRP assay had a half-life of 0.4 days in the absence of trehalose and a half-life of 1.1 days in the presence of trehalose. All three studies con fi rmed the enzyme-stabilizing e ff ect of trehalose, an ...
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Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris
... active HRP (Figure ...for HRP under the tested ...affect HRP productivity, the observed activity-enhancing trends seen for HEM1 and HEM3 were considered a consequence of the co-overexpressed HEM ...
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