HSV-TK
HSV-tk/GCV gene therapy mediated by EBV-LMP1 for EBV-associated cancer
... pVLTR- tk/GCV. It will be important to investigate the toxic effects of pVLTR-tk/GCV genes in normal ...the HSV-tk/GCV system may be used as a gene therapy strategy for EBV-LMP1 positive can- ...
7
Original Article Cell-killing effect of MAR regulating HSV-tk/GCV suicide gene system on ECA109 cells
... sequence. HSV-tk (For- ward): 5’-GCAAGAAGCCACGGAAGTCC-3’, HSV- tk (Reverse): 5’-CCCCGATATGAGGAGCCAGA-3’; MAR-HSV-tk (Forward): 5’-GAGTGAGTGTCAGG- CAGGTG-3’, ...
6
Original Article A fiber-modified adenovirus co-expressing HSV-TK and Coli.NTR enhances antitumor activities in breast cancer cells
... Due to both high levels of CAR and large endo- thelial pores to allow Ad vectors easy access to the liver, unwanted foreign gene transfer into this organ is a concern [10]. We therefore per- formed histopathological ...
11
Introduction of Three Independent Selection Markers in Leishmania
... the HSV-TK/GCV and CD/5-FC [20-22] negative selection systems inhibit the growth of promastigotes, the trans- fectants and mock transfected cells (electroporated in the absence of added DNA) were expanded ...
6
STAT3/NF-κB-Regulated Lentiviral TK/GCV Suicide Gene Therapy for Cisplatin-Resistant Triple-Negative Breast Cancer
... For HSV-tk/GCV suicide gene therapy, tumor microenvironment- specific promoters, such as redox-responsive promoter [21] and the NF-κB-based promoter system [43] have been designed for this ...
17
Ultrasound-targeted microbubble destruction mediated herpes simplex virus-thymidine kinase gene treats hepatoma in mice
... applied. HSV-TK is a prodrug enzyme gene which can express and produce TK in the tumor cells, catalyze nucleoside analogue to form mono- phosphate products, and further form a triphosphoric acid ...
6
Fiber-modified adenovirus-mediated suicide gene therapy can efficiently eliminate bladder cancer cells in vitro and in vivo
... gene HSV- TK could be highly expressed in bladder cancer cells under the control of the UPII ...RGDAd-UPII- TK alone control groups, the cells grew and proliferated well with a normal morphology ...
8
Rapid Phenotypic Characterization Method for Herpes Simplex Virus and Varicella Zoster Virus Thymidine Kinases To Screen for Acyclovir Resistant Viral Infection
... The TK genes of HSV-1, HSV-2, and VZV, under the control of a T7 promoter, were prepared by PCR using primer sets T7-HSV1-TK and HSV1- TK-Dw, T7-HSV2-TK and HSV2-TK-Dw, ...
6
Depletion of adult neurogenesis exacerbates cognitive deficits in Alzheimer’s disease by compromising hippocampal inhibition
... Third, we addressed the role of neurogenesis in regula- tion of the hippocampal circuit. It is thought that the DG regulates pattern separation either by encoding similar inputs with differential firing rates of place ...
13
Improved retroviral suicide gene transfer in colon cancer cell lines after cell synchronization with methotrexate
... the HSV-tk retroviral gene transfer resulted in an increase in GCV-mediated cell ...of HSV-tk gene reached 30% in the DHDK12 cell line 20 hr after MTX removal, doubling the efficiency of ...
12
Diverse Herpes Simplex Virus Type 1 Thymidine Kinase Mutants in Individual Human Neurons and Ganglia
... of tk mutant and wild-type ...of HSV tk mutants in animal neurons is ...mutated HSV tk sequences in human ...ACV-resistant HSV-1. The HSV-1 tk open reading frames ...
10
DNA-mediated gene transfer in Friend leukemia cells by cotransfection of simian virus 40 DNA with herpes simplex virus thymidine kinase DNA.
... Expression of SV40 T-antigen in FLCs after virus infection, and expression of SV40 T-antigen and HSV-TK gene after cotransfection with calcium phosphate-DNA precipitates in Friend leukem[r] ...
8
Development of a helper-independent human adenovirus vector and its use in the transfer of the herpes simplex virus thymidine kinase gene.
... To test the usefulness of this vector, the herpes simplex virus type 1 HSV-1 thymidine kinase gene tk along with its regulatory sequences was inserted into the unique XbaI site of dlEl,3[r] ...
8
Clearance of Herpes Simplex Virus Type 2 by CD8+ T Cells Requires Gamma Interferon and either Perforin- or Fas-Mediated Cytolytic Mechanisms
... ovalbumin-expressing HSV-2 virus (HSV-2 tk ⴚ OVA) from the genital epithelium of recipient mice, and clearance was abrogated by in vivo neutralization of gamma interferon (IFN- ␥ ...clear ...
9
DERIVATION OF TK- CLONES FROM REVERTANT TK+ MAMMALIAN CELLS
... In addition, we have examined a large number of BrdU’ clones independently selected from one TK+ revertant and several BrdU’ clones derived from many TK+ revertants in orde[r] ...
16
Activity of the simian virus 40 early promoter-enhancer in herpes simplex virus type 1 vectors is dependent on its position, the infected cell type, and the presence of Vmw175.
... The repression of SV40-cat in the BamHI z location appeared to require only those HSV-1 gene products expressed in the absence of Vmwl75, while repression of the transgene in the tk loca[r] ...
13
Identification of the herpes simplex virus DNA sequences present in six herpes simplex virus thymidine kinase-transformed mouse cell lines.
... The studies described in this paper were designed to both unambiguously map the HSV-1 and HSV-2 tk genes and to study the patterns of incorporation of non-tk gene viral DNA sequences in [r] ...
14
Evidence for a novel regulatory pathway for herpes simplex virus gene expression in trigeminal ganglion neurons.
... 5383 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Thymidine kinase TK-negative TK- mutant strains of herpes simplex virus type 1 HSV-1 show reduced expression of a an[r] ...
11
Mutant of herpes simplex virus type 1 conditionally able to transform thymidine kinaseless L cells to a tk+ phenotype.
... HSV mutant 1093 to grow in medium selective for the tk+ phenotype Ltk- cells were infected with UV-irradiated KOS 1.1 6, B2006, a tk- mutant of HSV-1 3, Transformed clones/well ata Thymi[r] ...
6
Factors Governing Expression of the Herpes Simplex Virus Gene for Thymidine Kinase in Clonal Derivatives of Transformed Mouse L Cells
... Because the synthesis of viral tk in cells productively infected with tk+ HSV-1 requires the prior synthesis of a proteins 8, 11, 32 and because expression of an a protein appears to be [r] ...
14