hTERT promoter
Xenoantigen, an αGal epitope-expression construct driven by the hTERT-promoter, specifically kills human pancreatic cancer cell line
... As described in Methods, SUSM-1 cells were shown to be negative for the telomerase-activity. The a Gal epitope was not detected on the cell surfaces of either naïve or NK7- transfected SUSM-1 cells (Fig. 3A,3B). Naïve ...
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Sequential combination therapy with flavopiridol and autocatalytic caspase-3 driven by amplified hTERT promoter synergistically suppresses human ovarian carcinoma growth in vitro and in mice
... amplified hTERT promoter system for efficient tran- scriptional targeting of active caspase-3 by using a two-step transcription amplification (TSTA) ...potent, hTERT-restricted apoptosis which is ...
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Latent Membrane Protein 1 of Epstein-Barr Virus Activates the hTERT Promoter and Enhances Telomerase Activity in B Lymphocytes
... of hTERT, the rate-limiting catalytic unit of the telomerase complex, was associated with the expression of the viral latent membrane protein 1 ...of hTERT transcripts, thus suggesting that LMP1 acts at the ...
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Targeting different types of human meningioma and glioma cells using a novel adenoviral vector expressing GFP-TRAIL fusion protein from hTERT promoter
... of hTERT promoter and they used these constructs for in vitro and in vivo anti-glioma studies via liposome delivery ...that hTERT/caspase constructs induced apoptosis in hTERT-positive ...
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Cell-Restricted Immortalization by Human Papillomavirus Correlates with Telomerase Activation and Engagement of the hTERT Promoter by Myc
... induces hTERT mRNA transcription in genital keratinocytes via interactions with Myc protein, thereby increasing cellular telomerase ...exogenous hTERT promoters and to immortalize genital keratinocytes and ...
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MR molecular imaging of tumors based on an optimal hTERT promoter tyrosinase expression system
... (hTERT) promoter or a cytomegalovirus(CMV) promoter in the hopes of performing noninvasive and real-time tumor-specific ...First, hTERT-TYR and CMV-TYR were constructed to infect cancer cell ...
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Synthetic Bax-Anti Bcl2 combination module actuated by super artificial hTERT promoter selectively inhibits malignant phenotypes of bladder cancer
... artificial hTERT promoter-driven re- porter module at ...artificial hTERT promoter could efficiently and selectively drive the expression of downstream gene in bladder cancer ...mutant ...
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The antiretroviral agent saquinavir enhances hTERT expression and telomerase activity in human T leukaemia cells in vitro
... on hTERT transcriptional control To further elucidate the involvement of a transcriptional mechanism underlying saquinavir- mediated increase of telomerase activity, we analyzed the binding of nuclear fac- tors to ...
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Telomere length regulation through epidermal growth factor receptor signaling in cancer
... decreasing promoter activity of ...with hTERT[63]. During the competition for hTERT promoter binding between HIF-1α and c-MYC, if there’s hypoxia, the promoter is predominantly bound by ...
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Oncolytic virotherapy in upper gastrointestinal tract cancers
... An armed oncolytic herpes simplex virus expressing thrombospondin-1 has an enhanced in vivo antitumor effect against human gastric cancer. Therapeutic efficacy of an hTERT promoter- dr[r] ...
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PubMedCentral-PMC5602382.pdf
... the hTERT promoter are known to be important for tight control of hTERT in normal tissues, but the molecular mechanisms leading to hTERT reactivation in cancer are not well-under- ...the ...
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RBFOX3 Promotes Tumor Growth and Progression via hTERT Signaling and Predicts a Poor Prognosis in Hepatocellular Carcinoma
... which hTERT is up-regulated in HCCs and promotes tumor growth and progression is not fully ...modulate hTERT signaling and HCC ...novel hTERT promoter-binding protein in HCC cells using ...
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A novel oHSV-1 targeting telomerase reverse transcriptase- positive cancer cells via tumor-specific promoters regulating the expression of ICP4
... the hTERT promoter to drive the expression of ICP4 from the the HSV-1 ...novel hTERT promoter-regulated oncolytic HSV-1 (oHSV1-hTERT), in which the ICP4 gene was controlled by the ...
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Diverse regulatory manners of human telomerase reverse transcriptase
... the hTERT promoter re- gion during telomerase ...mutant hTERT promoter without affecting that of the WT pro- moter, indicating that GABPA is the hTERT-regulating ETS factor that acts in ...
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RBFOX3 Promotes Gastric Cancer Growth and Progression through Activating HTERT Signaling
... ChIP assay was sone using Carey’s protocol. Briefly, cells were fixed with 1% formaldehyde and the cross-linking quenched with 100μl of 1.375 M glycine/mL of cell culture media. The samples were then sonicated on ice so ...
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Roles of telomeres and telomerase in cancer, and advances in telomerase-targeted therapies
... of hTERT activation in the initial stages of carcinogenesis, and the various genetic and epi- genetic mechanisms involved in its ...recurrent hTERT promoter mutations are highly frequent in many ...
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The correlations between DNA methylation and polymorphisms in the promoter region of the human telomerase reverse transcriptase (hTERT) gene with postoperative recurrence in patients with thyroid carcinoma (TC)
... The hTERT gene being the key component of telomerase catalytic activity, encodes the reverse transcriptase com- ponent of the telomerase complex, and its abnormity is considered to be associated to tumorigenesis ...
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Original Article MicroRNA-138 suppresses cell proliferation of human malignant melanoma cells by targeting hTERT
... hTERT 3’-UTR-full-length. MiR target site predic- tion for hTERT was performed using Target- scan Release 6.2, PicTar5and miRanda 3.0 programs. The wildtype hTERT3’-UTR was con- structed by PCR and inserted ...
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A critical role of nicotinamide phosphoribosyltransferase in human telomerase reverse transcriptase induction by resveratrol in aortic smooth muscle cells
... resistance, and longevity as reviewed by Guarente [23]. The majority of our knowledge of the SIRT family pertains to SIRT1, though the functions for the other SIRT genes, SIRT2–7, in mammalian physiology have recently ...
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Tumor cells derived exosomes contain hTERT mRNA and transform nonmalignant fibroblasts into telomerase positive cells
... that hTERT mRNA, the transcript of the enzyme telomerase, is shuttled from cancer cells via exosomes into telomerase negative fibroblasts, where it is translated into a fully active enzyme and transforms these ...
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