Huh7 cells
CD133+EpCAM+ Phenotype Possesses More Characteristics of Tumor Initiating Cells in Hepatocellular Carcinoma Huh7 Cells
... CD133, a 5-transmembrane domain glycopro- tein, expresses in normal [23] and tumor tissues. The function of CD133 is not entirely known yet, however it has been used as a marker of TICs in many solid tumors, including ...
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Increased susceptibility of Huh7 cells to HCV replication does not require mutations in RIG I
... the Huh7 cell clones, we transfected Huh7, Huh7D, and Huh7.5 cells with approximately 100 ng of the indi- cated HCV-hyg-Replicons and selected cells with 65 μg/ ml hygromycin ...Huh7.5 ...
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Bioinformatics analysis of hepatitis C virus genotype 2a-induced human hepatocellular carcinoma in Huh7 cells
... Abstract: Hepatocellular carcinoma (HCC) is a liver cancer that could be induced by hepatitis C virus genotype 2a Japanese fulminant hepatitis-1 (JFH-1) strain. The aim of this study was to investigate the molecular ...
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Original Article miR-21 is involved in resistance of Huh7 cells to cisplatin by regulating Wnt signaling via PTEN not CK1
... This present study further found that miR-21 targets PETN, not CK1, in Huh7 cells. Ma et al. reported that Wnt/β-catenin signaling was hyperactivated in metastatic breast cancer cells expressing ...
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Growth Inhibition of Hepatocellular Carcinoma Huh7 Cells by Lactobacillus casei Extract
... viability was compared and determined by inverted micros- copy (Olympus Model IX51, Tokyo, Japan), comprising a DP50 camera system (Olympus, Tokyo, Japan), using a 0.4% trypan blue dye exclusion method 72 hours after ...
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Linoleate appears to protect against palmitate-induced inflammation in Huh7 cells
... control, Huh7 cells incubated with bovine serum albumin) than those with palmitate treatment alone ...the cells treated by 500 μ M palmitate ...the cells treated by 500 μ M palmiate alone (n = ...
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Synthesis of hepadnavirus particles that contain replication-defective duck hepatitis B virus genomes in cultured HuH7 cells.
... Three types of evidence indicate that the ADR1 genome acts as a helper by providing, in trans, functions required for packaging a second genome: i fluorescent staining directly demonstra[r] ...
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USP15 Participates in Hepatitis C Virus Propagation through Regulation of Viral RNA Translation and Lipid Droplet Formation
... 293T cells were evaluated by immunoprecipitation (IP) with antibodies to FLAG (FL), HA, and Glu-Glu (control [C]) ...in Huh7 cells was observed by confocal ...USP15KOHuh7 cells were observed ...
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Relationships between Hepatitis C Virus Replication and CXCL-8 Production In Vitro
... BB7 cells that were mock transfected or transfected with CXCL8-1 siRNA were tested for the cytokines IL-1  , IL-6, and TNF- ␣ by fluorescent multiplex ELISA ...BB7 cells were transfected with 25 nM of the ...
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Nonstructural 3 Protein of Hepatitis C Virus Modulates the Tribbles Homolog 3/Akt Signaling Pathway for Persistent Viral Infection
... HEK293T cells were cotransfected with Flag-tagged TRIB3 and each Myc-tagged viral protein expression ...interaction, Huh7 cells harboring the HCV replicon derived from genotype 1b were transfected ...
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Neuralized E3 Ubiquitin Protein Ligase 3 Is an Inducible Antiviral Effector That Inhibits Hepatitis C Virus Assembly by Targeting Viral E1 Glycoprotein
... HEK293T cells were cotransfected with plasmids expressing Flag-tagged E1 and NEURL3, NEURL3-ΔRING, or NEURL3-ΔNHR, and cell lysates collected at day 2 posttransfection were immunoprecipitated with an anti-Flag ...
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TR4 nuclear receptor enhances the cisplatin chemo-sensitivity via altering the ATF3 expression to better suppress HCC cell growth
... in Huh7 cells (Figure 3A, mRNA level and protein level), and then treated these cells with cisplatin and applied MTS assay to analyze the cytotoxicity of these ...that Huh7 cells have ...
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Hepatitis C Virus Replication Is Modulated by the Interaction of Nonstructural Protein NS5B and Fatty Acid Synthase
... tions are significantly increased in patients with chronic HCV in- fection (38, 39). FASN is also upregulated in HCV-infected Huh7 cells (18). Accordingly, a significantly higher level of free fatty acid ...
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Silencing HOXD10 by promoter region hypermethylation activates ERK signaling in hepatocellular carcinoma
... Next, we detected phosphorylation status of ERK1/2 by western blot to further explore the mechanism of IGFBP3 in regulation of downstream signaling. After 24 h of serum starvation followed by 10% serum stimu- lation for ...
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CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation
... in Huh7 cells (Figure ...293T cells respectively, only the RNF41-CT mutant was able to precipitate with Flag-tagged CACYBP (Figure 3G), suggesting that CACYBP may be recruited by RNF41 as a substrate ...
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Hepatitis E Virus Replication Requires an Active Ubiquitin-Proteasome System
... underlined). Huh7 cells were transfected with the capped full-length RNA transcripts of the pSK-HEV2 infectious clone, and cells were treated with 1 M MG132 inhibitor at 1 day posttransfection and ...
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Visualisation and analysis of hepatitis C virus non-structural proteins using super-resolution microscopy
... infected cells reveals that NS5A and NS3 organise into ...in Huh7 cells (a hepatocarcinoma cell line), infected with the JFH-1 strain of hepatitis C virus for 24 h, showed that this protein localised ...
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Replication of the Human Hepatitis Delta Virus Genome Is Initiated in Mouse Hepatocytes following Intravenous Injection of Naked DNA or RNA Sequences
... from Huh7 cells at 5 days after transfection with the same DNA (as previously described [5]) and in lane 10 RNA extracted from the liver of an infected woodchuck (as previously described ...
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Paradoxical overexpression of MBNL2 in hepatocellular carcinoma inhibits tumor growth and invasion
... in Huh7 cells did not affect in vitro proliferation and anchorage-independent growth, it increased the migration and invasion ability of Huh7 ...tumor cells escape from this mechanism to form ...
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<p>LncRNA CDKN2B-AS1 Promotes Cell Viability, Migration, and Invasion of Hepatocellular Carcinoma via Sponging miR-424-5p</p>
... into Huh7 cells and MHCC97H cells, respectively, and the transfection ef fi ciency was ...in Huh7 cells but signi fi cantly high in MHCC97H cells (Figure 2A and ...of Huh7 ...
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