Human bone marrow mesenchymal stem cell (hMSC) isolation
BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation.
... the isolation of BMhMSC would simplify and enable direct cross-comparison to take place in the absence of distinct ...and isolation is glucose. Cell culture media utilized in the isolation of ...
42
A novel tsRNA-16902 regulating the adipogenic differentiation of human bone marrow mesenchymal stem cells
... 3T3-L1 cell line model, potentially indicating that our results are more physiologically ...regulate stem cell states in murine embryonic stem cells (mESCs), consistent with our findings ...
14
Original Article Isolation and characterization of mesenchymal stem cells from human bone marrow
... 3 bone fragment was immediately immersed in Dulbecco’s modi- fied Eagle’s medium (DMEM) in a 50 ml tube on ice for temporary ...the bone frag- ment was put into the clean bench as soon as ...spongy ...
7
Lipidomics for Human Bone Marrow Mesenchymal Stem Cells
... cleaves PIPs to produce diacylglycerol and inositol phosphate both of which can act as second messengers (Fisher & Jain 2009). 1.4 Lipidomics The previous scientific revolution eras, i.e. genomics and proteomics, have ...
76
An efficient method for isolation of murine bone marrow mesenchymal stem cells
... in cell proliferation and cell cycle progression has been ...the cell sorting technique for the isolation of ...a cell population exhibiting reduced clonogenicity and osteogenic ...
8
Equine Bone Marrow Derived Mesenchymal Stem Cells: Isolation and Multilineage Differentiation
... Bone marrow harvest Collection and isolation of eMSCs were performed by use of a modification of published protocols. 7,17 For collection, horses were moved to standing stocks and sedated. A 10cm ...
8
Mesenchymal stem cell alterations in bone marrow lesions in hip osteoarthritis
... BML bone despite a higher cross-sectional bone ...BML bone could also be attributable to the defect in the capacity of BML MSCs to regulate bone ...subchondral bone osteoblasts and ...
12
Original Article Effects of acidosis on the osteogenic differentiation of human bone marrow mesenchymal stem cell
... in human bone marrow mesenchymal stem cells (hBMMSCs) induced by simulated respiratory acidosis and the mechanisms underlying ...blotting. Cell proliferation was measured with ...
8
Age associated hydroxymethylation in human bone marrow mesenchymal stem cells
... Fig. 5 Chromatin signatures associated with DNA hydroxymethylation in MSCs of two different human age groups. a Heatmap showing the rela‑ tive enrichment of the hyper5hmC (upper) and hypo5hmC (lower) sites for a ...
14
Isolation of clinically relevant concentrations of bone marrow mesenchymal stem cells without centrifugation
... different bone marrow niches, further studies investi- gating the geographical location of different cell types within the marrow space and the influence of different aspiration protocols on ...
10
Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium
... native bone is still required to this day. Since human bone cells directly interact with nanostructured extracellular matrices, one of the most promising methods of improving titanium’s ...
12
Microbiota regulates bone marrow mesenchymal stem cell lineage differentiation and immunomodulation
... E Xiao 1,2 † , Linhai He 1,2 † , Qiong Wu 4,5 , Junxiang Li 4,5 , Yang He 1,2 , Lu Zhao 1,2 , Shuo Chen 1,2 , Jingang An 1,2 , Yansong Liu 6 , Chider Chen 3* and Yi Zhang 1,2* Abstract Health is dependent on the ...
7
Impact of bone marrow mesenchymal stem cell immunomodulation on the osteogenic effects of laponite
... potential bone biomaterial when evaluated with the traditional “one cell” strategy; however, this effect was attenuated when the evaluating system changed to a “multiple cell types” approach to ...
18
Bone marrow mesenchymal stem cells promote osteosarcoma cell proliferation and invasion
... local stem cells adjacent to osteosarcoma cells are involved in tumor growth and invasion may be useful for osteosar- coma therapy, as these results suggest that the bone marrow tissue surrounding ...
7
Comparative analysis of curative effect of bone marrow mesenchymal stem cell and bone marrow mononuclear cell transplantation for spastic cerebral palsy
... These findings are the same as those for previous studies (Alok Sharma et al. [4] using BMMNCs; Guojun Chen et al. using neural stem cell-like cells derived from autolo- gous BMMSCs) about the timing of ...
9
Aspirin promotes bone marrow mesenchymal stem cell-based calvarial bone regeneration in mini swine
... of bone disorders, such as periodontitis and ...in bone remodeling in the process of periodontitis, although the bacterial infection is thought to be one of the dominant ...MSCs-mediated cell therapy ...
11
Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig
... of bone marrow derived progenitor cells, the number of which increase rapidly following smoking cessation [7, ...profile. Bone marrow dys- function caused by constant CS exposure and failure ...
12
Hepatitis B virus infection and replication in human bone marrow mesenchymal stem cells
... of bone marrow stem cells, BMSCs are relatively easily separated and grown in cul- ...with human hepatocyte culture ...that human BMSCs are capable of differentiating into functional ...
8
Cajanine promotes osteogenic differentiation and proliferation of human bone marrow mesenchymal stem cells
... Bone marrow MSCs are maintained in a relative state of quiescence until they are activated by a variety of physi- ological and pathological ...stimuli. Bone marrow MSCs are capable of ...
6
The mechanism of miR-889 regulates osteogenesis in human bone marrow mesenchymal stem cells
... Methods: Osteoporosis patients and normal control bone tissues were collected and used PCR techniques to identify the change of miR-889 and WNT7A. Moreover, the dynamic change of miR-889 and WNT7A during ...
8