Human hepatocyte-like cells
Rapid generation of functional hepatocyte-like cells from human adipose-derived stem cells
... lineage cells, including adipocytes, osteocytes, and ...parenchyma cells in the context of fibrosis, cancer, or liver dysfunction and whether they pro- mote the development of these symptoms is still un- ...
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<p>In vitro Differentiation of Human TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells</p>
... E12 cells and cells at the various stages of differentia- tion were harvested by dissociation with Tryp-LE (12,605 – 028, Life Technologies, Grand Island, ...the cells for staining, cells were ...
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Hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study
... Transcriptomic characterization of the intermediate cells collected along the course of the differentiation re- vealed a dynamic nature of the process. On the PCA plot (Fig. 3), cell fate changed gradually from ...
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Development of Simple Protocol for Generation of Functionally Active Hepatocyte-like Cells from Human Adipose Tissue-derived Stem Cells
... Aims: Human adipose tissue-derived stem cells (hASCs) are considered as an attractive source of regenerative stem cells, mainly because of their higher proliferation rate, more accessibility and ...
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Identification and characterization of epithelial cells derived from human ovarian follicular fluid
... epithelial cells expresses germ-line stem cell markers, such as octamer-binding transcription factor 4 (OCT4), NANOG, and DEAD box polypeptide 4 ...oocyte-like cells formed spontaneously in vitro and ...
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A robust model of natural hepatitis C infection using hepatocyte like cells derived from human induced pluripotent stem cells as a long term host
... on human hepatocellular carcinoma cells (Huh7 and their deriva- tives) ...derived cells [12, ...primary human hepatocyte. The cancer cells actively entered cellular division ...
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Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte like cells
... ligands, cells were permeabilized with ...against human albu- min (rabbit polyclonal, 1:1,000, or mouse monoclon- al clone HAS-11, 1:1,000), human α FP (clone C3, 1:500), pan-CK (clone C-11, 1:400), ...
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Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening
... for human primary hepatocytes has increased ...these cells is limited by inadequate supply, high cost, and low in vitro proliferation ...these cells is still lost [7, ...primary cells, these ...
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Transplantation of human adipose stem cell-derived hepatocyte-like cells with restricted localization to liver using acellular amniotic membrane
... matrix, including a smooth and compact collagenous basement surface and a meshwork of collagenous fibrils and stroma, was preserved upon decellularization and cryopreservation (Additional file 2). Immunostaining analysis ...
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<p>Hepatic Differentiation of Marmoset Embryonic Stem Cells and Functional Characterization of ESC-Derived Hepatocyte-Like Cells</p>
... primary human hepato- ...to human antibodies 62–64 and the results from our studies, these marmoset HLCs might be ideal candidates to study modelling and cell therapy of human liver diseases; and for ...
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Analysis of differentially expressed genes among human hair follicle–derived iPSCs, induced hepatocyte-like cells, and primary hepatocytes
... the cells began to differen- tiate and the following day was considered to be day ...ES cells or iPSCs into HLCs, we divided the induction process into four ...specialized cells were cultured in the ...
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Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure
... express all of these pluripotent markers (Fig. 1e), indicating hAESCs have the capability of self-renew as well as multi- lineage differentiation potentials. Soft agar allows for the three-dimensional growth of cell ...
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Recapitulation of methotrexate hepatotoxicity with induced pluripotent stem cell-derived hepatocytes from patients with rheumatoid arthritis
... stem cells (iPSCs), which are induced by viruses expressing the Yamanaka factors (OCT4, Sox2, Klf4, c-Myc), can be generated from mul- tiple types of somatic cells, including fibroblasts and per- ipheral ...
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Generation of functional human hepatocytes in vitro: current status and future prospects
... maximum human ALB concentration was 728 μg/ml. In an attempt to generate cells that proliferate more rapidly than CFPHs and that exhibit a repopulative capacity and hep- atocyte functions after ...
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Efficient Differentiation of Human Induced Pluripotent Stem Cell (hiPSC) Derived Hepatocyte-Like Cells on hMSCs Feeder.
... of cells was ...pre-mature hepatocyte of monolayer culture, decreased in final period (Figure ...Undifferentiated cells did not secrete Urea whereas after induction of hepatic differentiation, urea ...
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Different approaches for transformation of mesenchymal stem cells into hepatocyte-like cells
... stem cells; SCs: Stem cells; ESCs: Embryonic stem cells; HLCs: Hepatocyte-like cells; ISCT: The International Society for Cellular Therapy; BM-MSCs: Bone marrow MSCs; AT-MSCs: ...
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Novel approaches using human induced pluripotent stem cells and microRNAs in the development of relevant human hepatocyte models for drug-induced liver injury
... optimal hepatocyte function which limits the utility of the hPH model to acute toxicity studies only, and compromises their use in mechanistic studies of DILI which often occurs following prolonged exposure to ...
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Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D
... biological cells (or bioprinting), tissue engineering was identi fi ed as a major application for this new technology [ 19 ] ...viable cells of multiple cell types into well- defined three-dimensional arrays ...
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Investigating novel methods of enhancing in vitro models of drug induced liver injury
... of hepatocyte-like cells from induced pluripotent stem cells ...iPSC-derived hepatocyte-like cells offer a reproducible, physiologically- relevant, genotypically normal ...
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The therapeutic potential of induced hepatocyte-like cells generated by direct reprogramming on hepatic fibrosis
... GFP-labeled cells were detected in control group (DMEM injection only) at 4 h after transplantation ...primary hepatocyte through observation of co-localization of al- bumin and GFP-labeled iHEPs by ...
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