Human Induced Pluripotent Stem Cell derived CMs
Deep phenotyping of human induced pluripotent stem cell–derived atrial and ventricular cardiomyocytes
... Discussion In this study, we applied simplified and efficient protocols for differentiation of human iPSCs into defined populations of atrial or ventricular CM subtypes based on monolayer and feeder-free culture ...
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GLUT4 expression and glucose transport in human induced pluripotent stem cell-derived cardiomyocytes
... Abstract Induced pluripotent stem cell derived cardiomyocytes (iPSC-CM) have the potential to trans- form regenerative cardiac medicine and the modelling of cardiac ...
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Human induced pluripotent stem cell derived neurons as a model for Williams-Beuren syndrome
... able human tissues, we differentiated WBS patient-specific induced pluripotent stem cells (iPSC) into cortical neurons for modeling the neuronal phenotype of ...iPSC derived neurons and ...
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Perspectives on the Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Biomedical Research
... the human heart. The advent of pluripotent stem cell technology that allows cardio- vascular cells to be derived from patients and healthy individ- uals, in some cases genetically ...
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Manifestation of Huntington’s disease pathology in human induced pluripotent stem cell-derived neurons
... recorded SOC currents in GMSLNs differentiated inde- pendently from various HD and WT cell lines. Subse- quently, HD neurons carrying 40–47 glutamine residues reproducibly exhibited 2-fold higher SOC currents ...
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Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes
... Overall, we have shown that the current platform enables quantitative prediction of the potential cardio- toxicity of drugs at different time points by analyzing the changes of CI, beating rate, and amplitude of ...
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Generation and Applications of Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells
... Copyright © 2018 Chengzhu Zhao and Makoto Ikeya. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, ...
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The advancement of human pluripotent stem cell derived therapies into the clinic
... cells derived with this protocol are not fully differentiated and require a maturation step in vivo to reach the definitive β -cell state in which they secrete insulin in response to increasing ...
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Effects of cellular origin on differentiation of human induced pluripotent stem cell–derived endothelial cells
... derived iPSCs before and after differentiation, using fetal primary ECs as a positive control for comparison. These genes included CTGF, HGF, VEGFB, KDR, NOS3, and PECAM1. Consistent with our hypothesis, EC-iPSCs ...
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Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection
... In particular, their effects on tuberculosis infection, espe- cially the immunological function in response to tubercu- losis infection, have not been thoroughly investigated. Thus, in the present study, we optimized the ...
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Cellular and molecular characterization of multiplex autism in human induced pluripotent stem cell-derived neurons
... the induced pluripotent stem cell (iPSC) model of severe ASD affectation was acquired) began to exhibit delays in development by 9 months of ...
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Predicting Induced Pluripotent Stem Cell Derived Cardiomyocytes Differentiation Outcomes
... Statement Induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) have revolutionized cardiovascular ...specific pluripotent stem cells (iPSCs), cells ...
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Induced pluripotent stem cell–derived cardiomyocytes in studies of inherited arrhythmias
... The last characterization of iPS-derived myocytes from LQTS patients was performed by Yazawa et al. in two individuals affected by Timothy syndrome (LQT8) (36). LQT8 is caused by mutations in the gene encoding the ...
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A safeguard system for induced pluripotent stem cell-derived rejuvenated T cell therapy
... SUMMARY The discovery of induced pluripotent stem cells (iPSCs) has created promising new avenues for therapies in regenerative medicine. However, the tumorigenic potential of undifferentiated iPSCs ...
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Deterministic HOX Patterning in Human Pluripotent Stem Cell-Derived Neuroectoderm
... a stem zone proximal to the primitive streak’s regressing node are the cell source for both the posterior neural tube and flanking paraxial mesoderm ( Delfino-Machı´n et ...the stem zone experience a ...
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Differentiation and transplantation of human induced pluripotent stem cell-derived otic epithelial progenitors in mouse cochlea
... ous pluripotent genes SOX2, KLF4, OCT4, and c-MYC in ESC-like colonies compared with that in urinary cells, indicating that transduction of exogenous genes could activate the expression of endogenous ...
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Cardiac repair in a murine model of myocardial infarction with human induced pluripotent stem cell-derived cardiomyocytes
... and cell therapy, there are several major hurdles that need to be addressed prior to their clinical ...in cell-based ...transplanted CMs also need to exhibit a mature phenotype similar to that ...
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2D versus 3D human induced pluripotent stem cell-derived cultures for neurodegenerative disease modelling
... into human induced pluripotent stem cells (hiPSCs), which can then be differentiated into any cell type for ...mimic human brain complexity, 3D models have been proposed as a ...
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Modeling Cardiovascular Risks of E-Cigarettes With Human-Induced Pluripotent Stem Cell-Derived Endothelial Cells.
... Despite its many strengths, our study has several limitations. First, as we were interested in assessing the direct effects of these liquids on vascular health, the results from our in vitro evaluation of e-liquid ...
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Establishment of human trophoblast stem cells from human induced pluripotent stem cell-derived cystic cells under micromesh culture
... cells derived from hiPSCs, for the first ...CTB stem-like cells from hiPSCs. However, these CTB stem-like cells were not proliferative, and their redifferentiation could not be controlled direction- ...
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