human induced pluripotent stem (iPS) cell
Human induced pluripotent stem cell-based microphysiological tissue models of myocardium and liver for drug development
... in stem cell biology and microfl uidic fabri- cation technology have established the foundation to develop organ-inspired high-throughput in vitro ...of human induced pluripotent ...
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Exosomes Secreted from Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Prevent Osteonecrosis of the Femoral Head by Promoting Angiogenesis
... by human-induced pluripotent stem cell-derived mesenchymal stem cells (hiPS-MSC-Exos) could promote angiogenesis of ischemic tissue in limb ischemia and skin defects [17, ...and ...
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Long-term Safety and Efficacy of Human-Induced Pluripotent Stem Cell (iPS) Grafts in a Preclinical Model of Retinitis Pigmentosa
... After 28 d of iPS differentiation, total cellular protein was extracted and ana- lyzed by sodium dodecyl sulfate– polyacrylamide gel electrophoresis. Sam- ples were then transferred to nitrocellu- lose membranes. ...
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Generation of human induced pluripotent stem cell-derived cardiomyocytes in 2D monolayer and scalable 3D suspension bioreactor cultures with reduced batch-to-batch variations
... into RNase free 1.5 tubes, washed by PBS (-/-), and centrifuged at 300 g for 2 minutes at 4 °C. The pellet was re-suspended in 1 mL TRIzol (Life technologies, # 15596026), repetitive pipetting and mixing on a vortex ...
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Induced pluripotent stem cells in the study of neurological diseases
... aforementioned cell types and follows the same steps described during normal embryonic development ...erentiating iPS cells into neurons is inhibition of pathways such as those of transforming growth factor ...
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Disruption of histamine/H1R signaling pathway represses cardiac differentiation and maturation of human induced pluripotent stem cells
... The in vitro cardiac differentiation from PSCs is simi- lar to heart development involving the primitive streak formation, the specification of the lateral mesoderm, and the generation of cardiac progenitor cells and ...
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Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation
... in iPS cells has been reported and may be associated with partial reprogramming ...favored iPS cells that retained expression of the indu- cing transgenes, and thus created partially-reprogrammed cells that ...
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Dynamic instability of genomic methylation patterns in pluripotent stem cells
... non- stem cells, which maintain methylation patterns in the absence of ...that human ES and induced pluripotent stem (iPS) cells tend to lose DNA methylation spontaneously in a ...
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A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement
... and cell pluripotent stem cell culture methods H9 human embryonic stem cells (hES) were purchased from WiCell (Madison, Wisconsin, ...USA). Induced pluripo- tent ...
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Successful disease-specific induced pluripotent stem cell generation from patients with kidney transplantation
... potent stem cells into specific renal cell types, or possi- bly a transplantable whole kidney, would enable the potential of cell therapy for ...of human ES cells into renal lineage expressing ...
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Temporal epigenetic modifications differentially regulate ES cell like colony formation and maturation
... to induced pluripotent stem (iPS) cells by forced expression of the transcription factors Oct4, Sox2, and either Klf4 and cMyc or Nanog and Lin28, using virus-based ...of iPS cells can ...
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Stem cells: a new paradigm for disease modeling and developing therapies for age related macular degeneration
... of stem cell biology for both disease modeling and therapeutic ...application. Human embryonic stem cells and induced pluripotent stem cells (iPSCs) have been used in ...
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Transplantation of induced pluripotent stem cell-derived mesenchymal stem cells improved erectile dysfunction induced by cavernous nerve injury
... Mesenchymal stem cells (MSC) are special cells with multi-lineage differentiation abilities ...for cell-based medical therapies because of their multi-lineage differentiation potential and ability to ...
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Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes
... drug- induced arrhythmias ...or human embryonic kidney cells, based on the two guidelines ICH S7B and ICH E14 [4, ...model cell lines, possessing interspecies differences in ion channels, biological ...
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The potential and limitations of induced pluripotent stem cells to achieve wound healing
... multiple cell types and ...therapies. Stem cell therapy is emerging as a potential treatment for chronic wounds, and adult-derived stem cells are currently employed in several commercially ...
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Pluripotent stem cells for Parkinson's disease: progress and challenges
... for cell replacement therapy. Initial eff orts focused on fetal cell transplantation, and signifi cant clinical benefi t lasting more than 10 years has been reported in some ...of human ...
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Human development, heredity and evolution
... in human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) technology have enabled the generation of human organoids (miniature versions of ...
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Understanding Megakaryopoiesis And Thrombopoiesis Using Human Stem Cells Models
... Human stem cell models (CD34+ hematopoietic progenitors, embryonic stem cells and induced pluripotent stem cells (iPSCs)) are powerful tools for the study of ...
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Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
... mesenchymal stem cells (MSCs) are known for their therapeutic potential and promising future in regenerative ...of human ASCs (hASCs), it is fundamental to compare their characteristics and understand how ...
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Polyethylene glycol in spinal cord injury repair: a critical review
... Figure 2 (A) Schematic for tethering peptides to PEG dimethacrylate gels. (B) Diagram of a syringe pump system used for embedding a linear gradient of peptide within a hydrogel. For two-dimensional cultures, only two ...
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