Human nucleus pulposus cells
Is Osteogenic Differentiation of Human Nucleus Pulposus Cells a Possibility for Biological Spinal Fusion?
... NP cells treated with osteogenic medium for 21 days, although the extent of staining varied considerably, between patients, both in number (from 10 to 80% of cells) and intensity ...in cells treated ...
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Original Article Resveratrol inhibits TNF-α-induced matrix degradation via the p38/MAPK and Akt pathways in human nucleus pulposus cells
... Abstract: Previous studies have proved that resveratrol has beneficial effects on intervertebral disc regeneration. Tumor necrosis factor-α (TNF-α) has been proven to induce matrix degradation, which plays a critical ...
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Biologic Response of Degenerative Living Human Nucleus Pulposus Cells to Treatment with Cytokines
... living human intervertebral discs ...recombinant human bone morphogenic protein (rh- BMP-2), transforming growth factor-β (TGF-β), interleukin-1β (IL-1β), and tumor necrosis factor-α ...of cells from ...
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Effects of TGF-β1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations
... rabbit nucleus pulposus at the 2-week time ...seed cells continue to be the problems, such as limited source, minor amounts and lower viabil- ...of human NP ...
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Original Article Expression of soluble Fas and soluble FasL in human nucleus pulposus cells
... in human nucleus pulposus (NP) and its attendant relationship with disc ...degeneration. Human NP samples were collected from patients with disc degeneration and cadavers as degenerate and ...
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Acidification changes affect the inflammasome in human nucleus pulposus cells
... CA) cells were obtained from the spinal column (isolated from the nu- cleus pulposus of human intervertebral discs) of a male donor that did not present spine degenerative ...disease. Cells ...
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Expression and regulation of neurotrophins in the nondegenerate and degenerate human intervertebral disc
... chondrocyte-like cells in all regions of the IVD and in all grades of ...cultured human nucleus pulposus cells, stimulation with IL-1β led to significant increases in NGF and BDNF gene ...
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Standardisation of basal medium for reproducible culture of human annulus fibrosus and nucleus pulposus cells
... AF and NP cells were expandable in all tested media. The different media did not affect the typical process of dedif- ferentiation known for cultured disc cells. Furthermore, both cell types showed same ...
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Original Article LincRNA-SLC20A1 (SLC20A1) promotes extracellular matrix degradation in nucleus pulposus cells in human intervertebral disc degeneration by targeting the miR-31-5p/MMP3 axis
... lated genes, such as long non-coding RNAs (lncRNAs), microRNAs (miRNAs) and protein coding genes, have been suggested [10, 11]. LncRNAs are longer than 200 nucleotides in length and have little protein coding potential. ...
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A complex interaction between Wnt signaling and TNF α in nucleus pulposus cells
... The Wnt-antagonizing activity of DKK-4 appears to be indistinguishable from that of DKK-1, whereas DKK-3 has distinct roles in regulating the Wnt pathway, depending on the cell types examined. For example, DKK-3 ...
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Intradiscal transplantation of synovial mesenchymal stem cells prevents intervertebral disc degeneration through suppression of matrix metalloproteinase related genes in nucleus pulposus cells in rabbits
... Paraffin-embedded sections were deparaffinized in xylene, rehydrated through graded alcohol, and immersed in PBS. The samples were pretreated with 0.4 mg/ml proteinase K (DAKO, Carpinteria, CA, USA) in Tris-HCl buffer ...
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Differential expression level of cytokeratin 8 in cells of the bovine nucleus pulposus complicates the search for specific intervertebral disc cell markers
... disc cells from other cartilage cells ...annulus cells, nucleus cells, and ACs, have found no specific markers, apart from ...the human disc nucleus ...vacuolated ...
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Co-culturing nucleus pulposus mesenchymal stem cells with notochordal cell-rich nucleus pulposus explants attenuates tumor necrosis factor-α-induced senescence
... stem cells (SCs), has been verified in human liver, skin, muscle, nervous system, heart, and bone [7, ...progenitor cells in the NP [9]; subsequently, several studies have proposed the exist- ence of ...
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Characteristics and potentials of stem cells derived from human degenerated nucleus pulposus: potential for regeneration of the intervertebral disc
... NPDCs adhered to the plastic surface of a tissue culture flask and were morphologically similar to BMSCs. However, the BMSCs showed slightly superior perform- ance with respect to proliferation capacity and colony- ...
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Expression and Role of Sphingosine 1-Phosphate Receptors in Intervertebral Disc Degeneration
... in human chondrocytes, S1P can inhibit the catabolic response induced by via activation of the S1P receptor[13, 14], thus the activation of the receptor may be a therapeutic target for ...of nucleus ...
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Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells
... of cells may represent promising therapy for treatment of disc degen- eration ...stem cells. Cell-based therapies by injection of IVD cells, chondro- cytes, or stem cells have gained ...
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Novel immortal human cell lines reveal subpopulations in the nucleus pulposus
... (Gibco). Cells were seeded at a density of 30,000 cells/cm 2 ...30,000 cells/cm 2 were incubated in dif- ferentiation medium (Dmed: DMEM/F-12, 1% antibiotic/ antimycotic, 1% ...
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The cytokine and chemokine expression profile of nucleus pulposus cells: implications for degeneration and regeneration of the intervertebral disc
... Four chemokines were selected for IHC investigation of protein production and localisation following LDA ana- lysis of gene expression. Expression of each target chemo- kine was investigated in 30 IVD tissue samples ...
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CD24 identifies nucleus pulposus progenitors/notochordal cells for disc regeneration
... adult human NP cell population is still unclear ...NP cells significantly decreased with in- creasing severity of disc ...notochordal cells has yet been identified, the findings of this study suggest ...
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The Human Inter Vertebral Disc - A Histological Approach
... term human intervertebral discs show the definite circular, concentrically arranged collagen fibers in anulus fibrosus and chondrocytes and degenerated notochordal cells in nucleus ...Notochordal ...
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