Human osteoarthritic chondrocytes
The unfolded protein response genes in human osteoarthritic chondrocytes: PERK emerges as a potential therapeutic target
... OA chondrocytes under H-UPR ...in chondrocytes is crucial as the type of collagen produced influences the quality of the cartilage ...OA chondrocytes could induce a decreased expression of type II ...
12
MicroRNA 125b regulates the expression of aggrecanase 1 (ADAMTS 4) in human osteoarthritic chondrocytes
... isolated chondrocytes were seeded in 75 cm 2 culture flasks and incubated in DMEM containing 10% fetal bovine serum (FBS), peni- cillin (100 U/ml), and streptomycin (100 mg/ml) at ...
11
Prostaglandin E2 regulates the expression of connective tissue growth factor (CTGF/CCN2) in human osteoarthritic chondrocytes via the EP4 receptor
... lated chondrocytes were then washed and cultured in vitro as a monolayer in Dulbecco ’ s modified Eagle ’ s medium supplemented with 10% fetal bovine serum and ...
6
Effects of low-intensity pulsed ultrasound and hyperbaric oxygen on human osteoarthritic chondrocytes
... The chondrocytes were released from the articular cartilage by sequential digestion with 1 mg/ml collage- nase (Sigma, ...isolated chondrocytes were centrifuged (1,000 rpm for 5 min), washed with PBS, and ...
6
Treatment with ephrin B2 positively impacts the abnormal metabolism of human osteoarthritic chondrocytes
... of human OA chondrocytes was prompted by various ...from human OA subchon- dral bone [12] suggest that this ephrin system could be tar- geted as a specific therapeutic approach in the development of ...
10
Transient receptor potential ankyrin 1 (TRPA1) is functionally expressed in primary human osteoarthritic chondrocytes
... man chondrocytes, to support the criteria set by Fer- nandes et ...primary human OA chondrocytes and in human T/C28a2 chon- drocyte cell ...
11
4 Hydroxynonenal induces apoptosis in human osteoarthritic chondrocytes: the protective role of glutathione S transferase
... Oxidative stress has been at the centre of the pathophysiolog- ical scene of OA disease over the past 30 years. Alterations in mitochondrial redox metabolism and respiratory functions may elicit the increased production ...
11
Investigation of the direct effects of salmon calcitonin on human osteoarthritic chondrocytes
... in human articular ...in human articular cartilage isolated directly without any prior cul- ...lated human chondrocytes pooled from four patients, and were in all instances able to detect ...
10
Regulation of the IGFBP 5 and MMP 13 genes by the microRNAs miR 140 and miR 27a in human osteoarthritic chondrocytes
... Many factors contribute to the overall degradation of car- tilage observed in osteoarthritis (OA), either directly or indirectly by modulating anabolic factors. Examples of such molecules are the matrix metalloprotease ...
11
The Novartis ILAR Rheumatology Prize 2001 Osteoarthritis: from molecule to man
... in osteoarthritic cartilage that is generated via an increase in the iNOS ...for osteoarthritic cartilage degradation, such as the reduction of ...sensitize human osteoarthritic ...
7
Edible Bird’s nest extract as a chondro protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study
... Prior ethical approval was obtained from the Research and Ethical Committee of Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM project code: 06-01-06- SF0257). Cartilage was obtained from six ...
9
Notochordal cell conditioned medium (NCCM) regenerates end stage human osteoarthritic articular chondrocytes and promotes a healthy phenotype
... on chondrocytes obtained from six patients (ages 60–82 years, all female), who suffered from end- stage OA and were undergoing total knee ...degraded osteoarthritic cartilage were collected and dir- ectly ...
11
Expression and function of K(ATP) channels in normal and osteoarthritic human chondrocytes: possible role in glucose sensing
... in human chondrocytes playing a role in the modulation of glucose transport capacity, we used glibenclamide, a widely used SUR inhibitor, to block these channels and measured the whole cell content of major ...
11
Preferential mRNA expression of prostromelysin relative to procollagenase and in situ localization in human articular cartilage
... late-stage osteoarthritic joints were lower than those in normal adult ...in chondrocytes of the superficial zone in adult ...treatment, chondrocytes in all cartilage zones were observed to express ...
10
Glycophenotyping of osteoarthritic cartilage and chondrocytes by RT qPCR, mass spectrometry, histochemistry with plant/human lectins and lectin localization with a glycoprotein
... using human chondrocytes in vitro and lectin histochemical analysis of clinical speci- ...two human adhesion/ growth-regulatory lectins, that is, Gal-1 and Gal-3, as histochemical ...
13
Original Article DCC is expressed in a CD166-positive subpopulation of chondrocytes in human osteoarthritic cartilage and modulates CRE activity
... in chondrocytes of three additional donors (Figure ...DCC-transfected chondrocytes versus controls which showed a significant reduction of CRE activity after trans- fection of DCC (Figure 2D), whereas NFkB ...
10
Protective effects of aucubin on osteoarthritic chondrocyte model induced by hydrogen peroxide and mechanical stimulus
... The expression of pro-inflammatory IL-6 inhibits the synthesis of aggrecan and is highly elevated in OA car- tilage [32]. IL-6 expression directly contributes to the inhibitory effect of aggrecan through the Notch recep- ...
11
DNA methylation regulates sclerostin (SOST) expression in osteoarthritic chondrocytes by bone morphogenetic protein 2 (BMP 2) induced changes in Smads binding affinity to the CpG region of SOST promoter
... OA chondrocytes but not in ...normal chondrocytes, suggesting that DNA methylation may impair the bind- ing of transcriptional factors, especially Smad binding in the SOST ...normal chondrocytes, ...
10
Expression of 92 kD type IV collagenase/gelatinase (gelatinase B) in osteoarthritic cartilage and its induction in normal human articular cartilage by interleukin 1
... stromelysin-1. Under identical conditions, mRNA levels for gelatinase A were not increased indicating that regulation of this enzyme in human articular chondrocytes is distinct from that of other ...
8
Effect of electromagnetic fields on human osteoarthritic and non osteoarthritic chondrocytes
... from human knee condyles of deceased patients after trauma devoid of involvement of the knee harvested within 12 h of death (n = 3) (together 5 male, 1 female; mean age 15; range 13 - 20) ...committee. ...
8