Human pancreatic islets
Induction of an Antiviral State and Attenuated Coxsackievirus Replication in Type III Interferon-Treated Primary Human Pancreatic Islets
... protect human pancreatic islets against coxsackievirus infection, and this is important since such viruses have been proposed to play a role in the development of human type 1 ...of ...
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Persistent Infection of Human Pancreatic Islets by Coxsackievirus B Is Associated with Alpha Interferon Synthesis in β Cells
... with human pancreatic islets from eight adult brain-dead donors were ...in human islets was proved by detection of viral RNA by in situ hybridization, VP1 capsid protein by ...
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Prolonged exposure of human pancreatic islets to high glucose concentrations in vitro impairs the beta cell function
... of human pancreatic islets to high glucose concentrations impairs the function of these ...purpose, islets isolated from adult cadaveric organ donors were cultured for seven days in RPMI 1640 ...
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Impairment of glucose induced insulin secretion in human pancreatic islets transplanted to diabetic nude mice
... rodent islets. To investigate the relevance of this hypothesis for human beta-cells in vivo, human pancreatic islets were transplanted under the ...
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Glucose induced β cell production of IL 1β contributes to glucotoxicity in human pancreatic islets
... cultured islets were fixed in 4% paraformaldehyde for 30 minutes at room temperature, followed by perme- abilization with ...Thereafter, islets were incubated with a guinea pig anti-insulin antibody as ...
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Glucose and GTP dependent stimulation of the carboxyl methylation of CDC42 in rodent and human pancreatic islets and pure beta cells Evidence for an essential role of GTP binding proteins in nutrient induced insulin secretion
... isolated islets was quantitated by batch-type static incubations or by perifusion of islets with test solutions (2, ...experiments, islets were placed into each of two chambers (100 islets per ...
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Effects of palmitate on genome-wide mRNA expression and DNA methylation patterns in human pancreatic islets
... DNA methylation reduces the transcriptional activity in functional in vitro studies [5,8]. Interestingly, here we find decreased expression in parallel with increased DNA methylation of several candidate genes for T2D, ...
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Human monoclonal autoantibodies that react with both pancreatic islets and thyroid
... of human peripheral blood lymphocytes with Epstein-Barr virus and rapid screening on rat insulinoma cells by an enzyme-linked immunosorbent assay were used to identify monoclonal autoantibodies that reacted with ...
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Reduction in Tcf7l2 Expression Decreases Diabetic Susceptibility in Mice
... in human TCF7L2 gene are known to be strongly asso- ciated with an increased risk of type 2 diabetes through the extensive genome-wide association studies in multiple ethnic populations ...the pancreatic ...
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Survival of isolated human islets of Langerhans maintained in tissue culture
... of human pancreatic islets to diabetic patients may require that donor islets be kept viable in vitro for extended time periods before transfer to the ...isolated pancreatic ...
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Human islets expressing HNF1A variant have defective β cell transcriptional regulatory networks
... donor islets contained β cells without insulitis and lacked glucose-stimulated insulin secretion despite a normal insulin response to cAMP-evoked ...of human pancreatic islets with a ...
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Memantine has no effect on KATP channels in pancreatic β cells
... isolated islets Next, we measured the amount of insulin secretion after memantine application using isolated ...to islets for this ...from pancreatic β cells in both glu- cose concentrations ...
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A low-protein diet during pregnancy prevents modifications in intercellular communication proteins in rat islets
... Background: Gap junctions between β -cells participate in the precise regulation of insulin secretion. Adherens junctions and their associated proteins are required for the formation, function and structural maintenance ...
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Prediction of spontaneous autoimmune diabetes in NOD mice by quantification of autoreactive T cells in peripheral blood
... that before this time, there was insufficient priming and/or proliferation of this autoreactive T cell popula- tion to allow visualization in blood. Since insulitis begins at approximately 3 weeks of age (25), these data ...
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Trimeprazine increases IRS2 in human islets and promotes pancreatic β cell growth and function in mice
... isolated human islets. Islets from 12 nondiabetic human cadaveric donors arrived over a period of 1 year in a controlled-tem- perature package maintained at 15°C to ...The islets were ...
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The effect of a novel curcumin derivative on pancreatic islet regeneration in experimental type-1 diabetes in rats (long term study)
... of pancreatic islets regeneration in response to treatment of diabetic rats with the NCD for forty ...and pancreatic environment to foster islet neo- ...
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Growth of Pancreatic Islets on Collagen Coated Textile Scaffolds.
... significant burden to both patients and society. Although the precise pathogenesis of T1DM is still unknown, scientists have found in genetically susceptible individuals, some environment factors that can trigger the ...
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Hepatic Autophagy in Uncontrolled Experimental Diabetes and Its Relationships to Insulin and Glucagon
... Exogenous glucagon is known to increase hepatic lysosomes, but the relationships between endogenous glucagon and insulin levels and hepatic lysosomes have not been examined. To determine if the hormones of the ...
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T cells expressing chimeric antigen receptor promote immune tolerance
... in human subjects has been effective in preventing graft-versus-host disease (GvHD), a life-threatening complication of hematopoietic stem cell transplantation involving donor cell–mediated immune attack of host ...
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Age dependent changes in the exocytotic efficacy in Kir6 2 ablated mouse pancreatic β cells
... pancreatic tissue slice preparation. This preparation is advantageous since it preserves socio-cellular context of the β-cells. Using this novel approach we revisited basic morphology and used whole-cell ...
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