human retinal pigment epithelium cells
Synergistic effects of gamma interferon on inflammatory mediators that induce interleukin-6 gene expression and secretion by human retinal pigment epithelial cells.
... INFLAMMATORY MEDIATORS AND IL-6 SECRETION BY RPE CELLS 577 human retinal pigment epithelium, p. Marmor (ed.), The retinal pigment epithelium[r] ...
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In Vitro Differentiation of Human Bone Marrow Stem Cells into Retinal Pigment Epithelium
... (Sigma-Aldrich). Cells were incubated for an additional 8 weeks with changing medium every 4 days and doing subculture every 10 days to prevent overgrowth of cells (Maria et ...
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Breaking barriers: insight into the pathogenesis of neovascular age-related macular degeneration
... relevant human heterotypic coculture and transmigration assays with magnified inserts on ...endothelial cells (CECs) are activated to migrate through Bruch’s membrane and make contact with the basal aspect ...
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Genetic ablation of retinal pigment epithelial cells reveals the adaptive response of the epithelium and impact on photoreceptors
... in human eye diseases we utilised the RPE CreER mice to genetically ablate RPE ...RPE cells) to assess changes in the ...RPE cells became rounded, lost their well defined ZO-1 staining and were ...
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A strategy to ensure safety of stem cell-derived retinal pigment epithelium cells
... RPE cells, we performed an unbiased screen for cell sur- face markers that were present exclusively on mature RPE but not on hESC or progenitor cells to enable effective depletion of these impurities by ...
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Protective effects of human iPS-derived retinal pigmented epithelial cells on retinal degenerative disease
... with retinal-inducing factors and RPE specification signaling ...hiPSC-RPE cells into the retina of rd10 ...hiPSC-RPE cells was detected by enzyme-linked immunosorbent assay ...the retinal ...
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Features specific to retinal pigment epithelium cells derived from three-dimensional human embryonic stem cell cultures — a new donor for cell therapy
... grafted cells clumped together in the subretinal ...3D-RPE-60d cells were quiescent 4 weeks post surgery (Figure ...3D-RPE-60d cells was thicker than that in opposite regions without transplanted ...
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Enhanced generation of retinal progenitor cells from human retinal pigment epithelial cells induced by amniotic fluid
... stem cells, and immortalized cell lines [16-19]. However, of all stem cells, retinal derived progenitor cells may be a more efficient treatment for vis- ual impairment ...of retinal ...
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Development of a blood retina barrier on a chip with human iPSC derived retinal pigment epithelium and endothelium
... these cells possess an altered gene and protein expression, lack pigmentation, and typical cobblestone morphology in standard culture ...research, human umbilical vein ECs (HUVECs) have been the “gold ...
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Expression profiling of cell-intrinsic regulators in the process of differentiation of human iPSCs into retinal lineages
... stem cells, such as ESCs and iPSCs, which are based on recap- itulating the pathways that lead to specification of retinal cell types during the natural development of an eye in an embryo ...primordial ...
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Original Article Plasticity of adult human retinal pigment epithelial cells
... Abstract: Retinal pigment epithelium (RPE) cell damage underlies many degenerative, dystrophic, and proliferative diseases of the human ...adult human RPE cells can be used for ...
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Abnormal mTORC1 signaling leads to retinal pigment epithelium degeneration
... All mice were housed in an Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC)-accredited specific-pathogen-free (SPF) animal facility (Model Animal Research Center, Nanjing University, ...
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Resveratrol inhibits transforming growth factor-β2-induced epithelial-to-mesenchymal transition in human retinal pigment epithelial cells by suppressing the Smad pathway
... of cells was tested using 4-[3-(4-iodophenyl)- 2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1; Roche Diagnostics, Indianapolis, IN, USA) assay, according to the manufacturer’s ...monolayer ...
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Monozygotic twins with polypoidal choroidal vasuculopathy
... Laser photocoagulation was applied to the polypoidal lesions at the edge of the PED, leading to the disappearance of the PED. However, dye leakage from the macular lesion further deteriorated the vision postoperatively. ...
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Correlation between peripapillary retinal nerve fiber layer thickness and fundus autofluorescence in primary open-angle glaucoma
... Only eyes suffering from POAG were included, defined by a documented history of elevated intraocular pressure, characteristic optic nerve head damage, and/or characteristic visual field defects. Exclusion criteria were ...
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Autophagy activated via GRP78 to alleviate endoplasmic reticulum stress for cell survival in blue light-mediated damage of A2E-laden RPEs
... ARPE-19 cells in this study may exist some limitations, as in current understanding, ARPE-19 cells only partially re- capitulate the features of human ...primary human RPEs or in vivo models ...
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Ion channels of cultured human retinal pigment epithelial cells
... cultured human RPE cells. These cells were chosen for several ...cultured cells had been used for single channel recording because the dissociation procedure required for good culture leave ...
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The advancement of human pluripotent stem cell derived therapies into the clinic
... grafted cells be immunologically ...endodermal cells and a proprietary encapsulation device that prevents immune cells from contacting the engrafted ...encapsulated cells, but optimizing the ...
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Knobloch syndrome associated with Polymicrogyria and early onset of retinal detachment: two case reports
... Our findings add to the literature supporting the spectrum of brain anomalies observed with KS, includ- ing polymicrogyria. Additionally, our cases are consistent with other reported cases of KS with polymicrogyria in ...
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Long-term success of intravitreal bevacizumab for choroidal neovascularization associated with choroidal osteoma
... the retinal sensitivity of the inferior nasal side probably decreased to lower the average ...the retinal sensitivity ...and retinal sensitivity may not be too surprising in some ...the ...
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