human tau
Apoptosis in Transgenic Mice Expressing the P301L Mutated Form of Human Tau
... of human tau, containing the frontotemporal dementia-associated P301L mutation, can be suppressed with doxycycline administration ...peat human tau with the P301L mutation placed downstream of ...
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Effects of the C57BL/6 strain background on tauopathy progression in the rTg4510 mouse model
... 0N4R tau as rTg4510 mice, there are sev- eral important differences between the models that may explain the disparate effects of the C57BL/6 strain on tauopathy in each ...The tau transgenic integration ...
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Multiple signaling factors and drugs alleviate neuronal death induced by expression of human and zebrafish tau proteins in vivo
... of tau protein by caspases and other prote- ases has been previously reported; purified recombinant human tau proteins truncated after Glu 391 or Asp 421 (equivalent to h4R-tau-△392 and ...
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Assembly of transgenic human P301S Tau is necessary for neurodegeneration in murine spinal cord
... of human Tau (residues 244–372), into filaments ...of Tau fila- ...expressing human wild-type Tau (0N4R) lacking residues 306–311 that developed no detectable neurodegeneration and sig- ...
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Generation of a new transgenic mouse model for assessment of tau gene silencing therapies
... To detect transgenic human tau expression in brain, mice were deeply anesthetized and then perfused with ice-cold PBS. The brains were then quickly removed and the forebrains were dissected and flash frozen ...
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What we can learn from animal models about cerebral multi-morbidity
... (and tau) may be the more neurotoxic species, Larson and colleagues [73] assessed the putative role of soluble α-synuclein in ...of human tau is required for the regulation of α-synuclein expression, ...
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N368-Tau fragments generated by legumain are detected only in trace amount in the insoluble Tau aggregates isolated from AD brain
... For non-targeted LC-MS/MS analysis, bands of inter- est were excised, and in-gel digested with trypsin. In brief, gel bands were reduced with 1.5 mg/mL DTT, alkylated with 10 mg/mL iodacetamide and digested with 6.25 ...
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3D Visualization of the Temporal and Spatial Spread of Tau Pathology Reveals Extensive Sites of Tau Accumulation Associated with Neuronal Loss and Recognition Memory Deficit in Aged Tau Transgenic Mice
... of human tau that is associated with neurofibrillary tangle (NFT) formation ...MC1-positive human tau protein, images of 12 intact sections from each mouse were captured under 4x objective and ...
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Late-stage Anle138b treatment ameliorates tau pathology and metabolic decline in a mouse model of human Alzheimer’s disease tau
... six human tau isoforms (hTau), thus consti- tuting a murine model of AD tauopathy ...of tau aggregates, thus emulating a likely clinical ...of tau pathology after 3 months ’ treatment with ...
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Phosphorylation of tau at Y18, but not tau-fyn binding, is required for tau to modulate NMDA receptor-dependent excitotoxicity in primary neuronal culture
... that tau plays a critical role in NMDAR-dependent excito- ...of human tau caused excitotoxicity in neuronal cultures ...increased tau expression [14, 15] and phosphorylation ...of tau ...
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Intersection of pathological tau and microglia at the synapse
... extracellular tau, and aggre- gated or hyperphosphorylated tau is observed in micro- glia of mice and humans with tau pathology [37, 38, 44, 73, 99, 192, 196, 216, 229, ...aggregated tau [44, ...
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A validated antibody panel for the characterization of tau post-translational modifications
... about tau modifications in human ...the tau PTM landscape and also facilitate the visualization of potential or proven interactions between PTMs, we de- signed an interactive tool, which comprises ...
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High copy wildtype human 1N4R tau expression promotes early pathological tauopathy accompanied by cognitive deficits without progressive neurofibrillary degeneration
... of tau and are specific for pathological ...of tau in- clude AT180 (pThr231; Thermo Scientific), AT270 (pThr181; Innogenetics), and PHF1 (pSer396/pSer404; provided by Peter Davies) ...labels tau ...
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Propagation of Tau aggregates
... expressing human P301S Tau with inclusions into mice transgenic for wild-type human Tau lacking inclusions (line ALZ17) induced the assembly of wild- type Tau into filaments and its ...
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Acetylated tau destabilizes the cytoskeleton in the axon initial segment and is mislocalized to the somatodendritic compartment
... Ac-K274 tau inclusions in human tauopathy ...ac-K274 tau immunostaining in the inferior temporal cortex of human tauopathy ...Cytoplasmic tau inclusions (arrows) in the brain of an ...
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Analysis of in vivo turnover of tau in a mouse model of tauopathy
... expressing human full-length tau cDNA (Tau 2N4R) with mutations ΔK280/PP (I277P, I308P) (anti-aggregant mice) and mice expressing hu- man full-length tau cDNA (Tau 2N4R) with a ΔK280 ...
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Changes of tau profiles in brains of the hamsters infected with scrapie strains 263 K or 139 A possibly associated with the alteration of phosphate kinases
... of tau, p-tau (Ser396), p-tau (Ser404), p-tau (Ser202/Thr205), GSK3β and CDK5 in the brain tissues of normal and scrapie 263K-infected hamsters during incubation ...of tau, p-tau ...
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Suppression of LPS-induced tau hyperphosphorylation by serum amyloid A
... on tau phosphorylation at T205 and S396 after incubation with CM from SAA-stimulated primary microglia ...of tau phosphorylation at T205 and S396 was determined by Western blotting using specific ...
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Slowly progressive dementia caused by MAPT R406W mutations: longitudinal report on a new kindred and systematic review
... A systematic review of the R406W literature was per- formed by searching the PubMed database (latest search date 24 February 2017). Search terms used were “tau”, “MAPT” or “Microtubule associated protein ...
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Comparing 18F-AV-1451 with CSF t-tau and p-tau for diagnosis of Alzheimer disease
... CSF samples were collected via lumbar puncture and analyzed for total tau (t-tau) and phosphorylated tau (p-tau) with standard pro- cedures. PET scans were used to measure 18 F-AV-1451 ...
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