Human type II collagen
Type II collagen and glycosaminoglycan expression induction in primary human chondrocyte by TGF β1
... of type I collagen coated 24 well ...recombinant human TGF-β1 (25 ng/ mass if not designated, eBioscience, San Diego, CA, USA), purified human type II collagen (25 ng/ ...
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Exposure to Mimivirus Collagen Promotes Arthritis
... mouse collagen type II and was accompanied by T-cell reactivity to collagen and joint inflammation, as observed in collagen-induced arthritis following immunization of mice with bovine ...
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Immunohistochemical detection and immunochemical analysis of type II collagen degradation in human normal, rheumatoid, and osteoarthritic articular cartilages and in explants of bovine articular cartilage cultured with interleukin 1
... cartilage collagen by developing immunochemical methods that permit the identification and analysis of type II collagen degradation in ...identify type II collagen ...
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Early and stable upregulation of collagen type II, collagen type I and YKL40 expression levels in cartilage during early experimental osteoarthritis occurs independent of joint location and histological grading
... of collagen type II, collagen type I and YKL40 expression, which persisted throughout the ...elevated collagen type II, collagen type I and YKL40 mRNA ...
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Interleukin 1 suppresses expression of cartilage specific types II and IX collagens and increases types I and III collagens in human chondrocytes
... recombinant human IL-1 beta suppressed synthesis of cartilage-specific type II collagen by cultured human costal chondrocytes associated with decreased steady state levels of alpha 1 ...
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Decreased hypertrophic differentiation accompanies enhanced matrix formation in co cultures of outer meniscus cells with bone marrow mesenchymal stromal cells
... of type I col- lagen throughout, type II collagen in the inner meniscus, and proteoglycans, of which aggrecan is predominant ...In human meniscus, the capillary plexus supplies only the ...
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Patella Alta and Trochlea Dysplasia Is Associated with Abnormal Type II Collagen and Matrix Accumulation in Chondrocytes
... in type II collagen, transmitted in an autosomal dominant manner from the mother to son and ...abnormal type II collagen heterofibrils and the putatively inherited dysplastic ...
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Biologic Treatment of Mild and Moderate Intervertebral Disc Degeneration
... and collagen type II synthesis ...increased collagen type II mRNA levels after being inserted into a rat intervertebral disc encapsu- lated in polylactic glycolic acid (PLGA) ...
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Type I collagen gene suppresses tumor growth and invasion of malignant human glioma cells
... Human type I collagen cDNA (HCOL1A1) was transfected into the human malignant glioma cell line ...of type I collagen (data not ...
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Disrupting the Indian hedgehog signaling pathway in vivo attenuates surgically induced osteoarthritis progression in Col2a1 CreERT2; Ihhfl/fl mice
... Gli2, type X collagen (the typical hypertrophic chondrocyte marker) and other cartilage-degrading enzymes, such as MMPs and ca- thepsins are effectively downregulated by Ihh deletion (Figures 3, 5 and ...
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Matrilin 3 Induction of IL 1 receptor antagonist Is required for up regulating collagen II and aggrecan and down regulating ADAMTS 5 gene expression
... of type II collagen protein, primary human chondrocytes transfected with either the IL-1Ra-silencing siRNA or the non-silencing scrambled control siRNA, were seeded and cultured for 48 hours ...
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The trans-well coculture of human synovial mesenchymal stem cells with chondrocytes leads to self-organization, chondrogenic differentiation, and secretion of TGFβ
... follows: collagen type I (mouse monoclonalAb COL-1; abcam, Cambridge, UK) 1:500, collagen type II (rabbit polyclonalAb COL-2; abcam) 1:250, and collagen type X (mouse ...
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Prostaglandin PGE2 at very low concentrations suppresses collagen cleavage in cultured human osteoarthritic articular cartilage: this involves a decrease in expression of proinflammatory genes, collagenases and COL10A1, a gene linked to chondrocyte hypertrophy
... collagenase-cleaved type II collagen The OA cartilage explants from day 16 of culture were digested and extracted with α-chymotrypsin to solubilize denatured collagen including the ...
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Cloning, expression, and type II collagenolytic activity of matrix metalloproteinase 13 from human osteoarthritic cartilage
... As has been observed previously for MMP-1 in chondro- cytes (10, 17), treating cartilage with a cytokine substantially upregulated the expression of mRNA for both MMP-1 and MMP-13. Antibodies to both MMP-1 and MMP-13 ...
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Molecular and ultrastructural studies of a fibrillar collagen from octocoral (Cnidaria)
... ehrenbergi collagen fibers and mammalian type I (rat) and type II (human) collagen enables the systematic application of crystallographic information collected from the latter to ...
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Type 1 regulatory T cells specific for collagen type II as an efficient cell based therapy in arthritis
... of human Treg cells, there is no consensus to date about which Treg population is op- timally suited for their various clinical ...in human disease. The use of Tr1 cells in human models of disease is ...
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Hormone replacement therapy, calcium and vitamin D3 versus calcium and vitamin D3alone decreases markers of cartilage and bone metabolism in rheumatoid arthritis: a randomized controlled trial [ISRCTN46523456]
... COMP is a 524-kDa, homopentameric, extracellular-matrix protein and it constitutes 0.5–1% of the wet weight of car- tilage and is released from cartilage during the erosive process [26]. Its biological function is still ...
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Effect of angiotensin II receptor blocker on TGF-β1, MMP-1, and collagen type I and type III concentration in New Zealand rabbit urethral stricture model
... and collagen types I and III concentrations were evaluated by ...rabbit collagen concentration in serum, plasma, and tissue homogenates, we used collagen type I ELISA Kit (CSB-E06858Rb) and ...
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Humoral and Cellular Sensitivity to Collagen in Type II Collagen Induced Arthritis in Rats
... developed type-specific cellular as well as humoral ...was type II. The latter collagen induced brisk cellular and humoral responses that were equivalent whether complete Freund's adjuvant or ...
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Collagen and Collagen derived Fragments Are Chemotactic for Tumor Cells
... in collagen such as liver, lungs, and bone are frequently sites of tumor cell ...of human and rat origin migrated unidirectionally in response to collagen in ...the collagen helix caused ...
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