HYDROLASE FAMILY
Comparative Community Proteomics Demonstrates the Unexpected Importance of Actinobacterial Glycoside Hydrolase Family 12 Protein for Crystalline Cellulose Hydrolysis
... glycoside hydrolase family 6 (GH06_exo), an endoglucanase from GH family 6 (GH06_endo), a cellobiohydrolase from GH family 48 (GH48 protein), a lytic polysaccharide monooxygenase of the ...
9
Natural diversity of glycoside hydrolase family 48 exoglucanases: insights from structure
... glycoside hydrolase family 48 are believed to be an important member of many bacterial cellulase systems which also lack the better studied GH6 and GH7 family cellobiohydrolases produced by most ...
9
In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions
... Results: Combined bioinformatic and transcriptomic analyses implicated three glycoside hydrolase family 5 subfam- ily 4 (GH5_4) members, with distinct modular organization, as potential keystone ...
16
Characterization of Glycoside Hydrolase Family 5 Proteins in Schizosaccharomyces pombe
... this family; Cps1/Bgs1, Bgs3, and Bgs4 are essential for cell viability during vegetative growth (14, 15, 29, 33), while Bgs2 performs an essential role during spore wall formation (27, ...side hydrolase ...
11
Spiro-epoxyglycosides as activity-based probes for glycoside hydrolase family 99 endomannosidase/endomannanase
... Abstract: N-Glycans direct protein function, stability, folding and targeting, and influence immunogenicity. While most glycosidases that process N-glycans cleave a single sugar residue at a time, enzymes from glycoside ...
10
Structural Enzymology of Cellvibrio japonicus Agd31B Protein Reveals α-Transglucosylase Activity in Glycoside Hydrolase Family 31
... glycoside hydrolase family 13 (GH13) and GH77 play well established roles in ␣ -glucan side chain (de)branching, regulation of oligo- and polysaccharide chain length, and formation of cyclic ...
18
Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1
... to members of other GH13 subfamilies. Currently, 42 sub- families of GH13 have been defined, but several sequences and characterized enzymes are not yet assigned to a sub- family [4]. Generally, GH13 subfamilies ...
11
A constitutive expression system for glycosyl hydrolase family 7 cellobiohydrolases in Hypocrea jecorina
... To examine this concern specifically, we performed a time-course growth of JLT102A coupled with multiplex Western blot using antibodies directed towards both Cel7A and Cel6A ( cbhII ) to[r] ...
12
Glycoside hydrolase family 32 is present in Bacillus subtilis phages
... in the CAZy database (http://www.cazy.org/) showed the presence of one Bacillus phage phiNIT1 sequence (accession AP013029.1) in the section of GH32 family. To determine if other homolog sequences exist in other ...
6
Enhancement of active compound, genipin, from Gardeniae Fructus using immobilized glycosyl hydrolase family 3 β-glucosidase from Lactobacillus antri
... Using 0.5 μg/mL free rBGLa, single compound of 0.4 mM geni‑ poside was efficiently converted into genipin within 2 h, and the immobilized rBGLa also successfully transformed geniposide [r] ...
8
The genes encoding glycoside hydrolase family 6 and 7 cellulases from the brown-rot fungus Coniophora puteana
... glycoside hydrolase (GH) family 6 and 7 cellulases (cel6A, cel6B, cel7A, and cel7B) were obtained from the brown-rot fungus Coniophora puteana by genomic polymerase chain reaction (PCR) using consensus ...
5
In vitro and in vivo characterization of three Cellvibrio japonicus Glycoside Hydrolase Family 5 members reveals potent xyloglucan backbone-cleaving functions
... family [28], subfamily 4 is the only subfamily known to contain predominant endo- xyloglucanases [23], which suggested a keystone role for this enzyme in xyloglucan utilization by C. japonicus. Notably, CJA_2477 ...
53
Characterization of β-N-acetylhexosaminidase (LeHex20A), a member of glycoside hydrolase family 20, from Lentinula edodes (shiitake mushroom)
... We purified and cloned a β -N-acetylhexosaminidase, LeHex20A, with a molecular mass of 79 kDa from the fruiting body of Lentinula edodes (shiitake mushroom). The gene lehex20a gene had 1,659 nucleotides, encoding 553 ...
7
Structure and function of a glycoside hydrolase family 8 endoxylanase from Teredinibacter turnerae
... xylanase-containing family GH30 (all of these glycoside hydrolase families are reviewed in CAZypedia; for a review, see The CAZypedia Consortium, ...to family GH8, a cellulase/ xylanase-containing ...
11
Distribution and evolution of glycoside hydrolase family 45 cellulases in nematodes and fungi
... The phylogenetic trees of nematode GH45 sequences showed a more complex structure than those of the fungi. The fact that several different copies of the genes are present within each individual nematode makes it ...
12
Structure and function of a glycoside hydrolase family 8 endoxylanase from Teredinibacter turnerae
... Given the known specificities of family GH8 members (available at http://www.cazy.org/GH8.html), chitosanase (EC 3.2.1.132), cellulase (EC 3.2.1.4), licheninase (EC 3.2.1.73), endo-1,4--xylanase (EC 3.2.1.8) and ...
10
Contribution of a family 1 carbohydrate-binding module in thermostable glycoside hydrolase 10 xylanase from Talaromyces cellulolyticus toward synergistic enzymatic hydrolysis of lignocellulose
... Results: Thermostable endo- β -1,4-xylanase (Xyl10A) from Talaromyces cellulolyticus, which is recognized as one of the core enzymes in the fungal cellulase system, has a modular structure consisting of a glycoside ...
13
Expression, purification, crystallization and preliminary X-ray diffraction analysis of conjugated bile salt hydrolase from Bifidobacterium longum
... et al., 1999), BSH from B. longum can be classi®ed as belonging to the N-terminal nucleophile hydrolase family (Brannigan et al., 1995), with Cys as the N-terminal catalytic residue. The majority of the ...
5
Complete genome sequence of bile-isolated Enterococcus avium strain 352
... bsh genes encoding bile salt hydrolase with a protein sequence identity of 92.9% in the genome of the E. avium strain 352. This result indicated that the BSH might be play an important role in niche-specific ...
5
A Review on Engineering of Organophosphorus Hydrolase (OPH) Enzyme
... Releasing toxic compounds as pesticides and insecticides, soluble, exploding, cooling, and dying materials in indus- try and agriculture to the environment has increased in recent decades. One of these synthesis ...
10