Hyperthermophilic archaeon
Distinct Physiological Roles of the Three Ferredoxins Encoded in the Hyperthermophilic Archaeon Thermococcus kodakarensis
... Redox reactions and electron flux are critical to all metabolisms. Fds—an ancient class of Fe-S-containing small proteins—are the most widely distributed and prevalent proteinaceous electron carriers (20, 21). Almost all ...
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The draft genome of the hyperthermophilic archaeon Pyrodictium delaneyi strain hulk, an iron and nitrate reducer, reveals the capacity for sulfate reduction
... of hyperthermophilic organisms ...model hyperthermophilic archaeon Geoglobus ahangari demonstrated the need for direct contact between the cell and the iron mineral to transfer respiratory electrons, ...
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The TK0271 Protein Activates Transcription of Aromatic Amino Acid Biosynthesis Genes in the Hyperthermophilic Archaeon Thermococcus kodakarensis
... a hyperthermophilic archaeon that displays an optimal growth temperature of 85°C (27, ...this archaeon, including glycolysis/gluconeogenesis, pentose metabolism, chitin degradation, and coenzyme A ...
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Lesion-Induced Mutation in the Hyperthermophilic Archaeon Sulfolobus acidocaldarius and Its Avoidance by the Y-Family DNA Polymerase Dbh
... polymerase implicated in TLS, and two additional B-family enzymes that remain relatively obscure. Biochemical studies of the latter enzymes of S. solfataricus indicate limited affinity for DNA substrate, limited ...
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Superoxide Reductase from the Hyperthermophilic Archaeon Pyrococcus furiosus: its Function, Regulation, and Biotechnological Applications
... anaerobic hyperthermophilic archaeon, Pyrococcus furious, possesses a system for the detoxification of reactive oxygen species, which is different from the classical defense mechanisms present in ...
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Extragenic Pleiotropic Mutations That Repress Glycosyl Hydrolase Expression in the Hyperthermophilic Archaeon Sulfolobus solfataricus
... The hyperthermophilic archaeon Sulfolobus solfataricus employs a catabolite repression-like regulatory system to control enzymes involved in carbon and energy ...
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Characterization of a Thermostable, Recombinant Carboxylesterase from the Hyperthermophilic Archaeon Metallosphaera sedula DSM5348
... Effect of pH on the activity of purified Msed_1072 gray bar, Msed_1072Nt white bars, and Msed_1072Ct black bars was determined over a pH range from 4 to 10, at the optimal temperature fo[r] ...
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Functional Characterization of MoeA and MoeB Tungsten Cofactor Synthesis Proteins from the Hyperthermophilic Archaeon Pyrococcus furiosus
... proteolytically on peptides and has an optimal growth temperature of 100°C (Fiala and Stetter, 1986). By analysis of 16S ribosomal RNA, it was determined that this organism can be classified as an Archaea (formerly ...
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Functional Evaluation of the Mechanisms of Reactive Oxygen Detoxification in the Hyperthermophilic Archaeon Pyrococcus Furiosus Using Complementation Studies
... It is known that anaerobic microorganisms can periodically be exposed to oxygen in their environments, and therefore, having some protective mechanism to detoxify reactive oxygen species would be highly advantageous for ...
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Engineering a Hyperthermophilic Archaeon for Temperature Dependent Product Formation
... IMPORTANCE Extremely thermostable enzymes from microorganisms that grow near or above the boiling point of water are al- ready used in biotechnology. However, the use of hyperthermophilic microorganisms themselves ...
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Hyperthermophilic endoglucanase for in planta lignocellulose conversion
... the hyperthermophilic archaeon Sulfolobus solfa- taricus encodes three different endoglucanases (SSO1354, SSO1949 and SSO2534) [20] that have already shown their potential to perform efficiently under harsh ...
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CRISPR mediated targeted mRNA degradation in the archaeon Sulfolobus solfataricus
... The recently discovered clustered regularly interspaced short palindromic repeat (CRISPR)- mediated virus defense represents an adaptive immune system in many bacteria and archaea. Small CRISPR RNAs cause cleavage of ...
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Expression and biochemical characterization of maltogenic amylase from bacillus lehensis G1
... Li, D., Park, J. T., Li, X., Kim, S., Lee, S., Shim J. H., Park, S. H., Cha, J., Lee, B. H., Kim, J. W. and Park, K. H. (2010). Overexpression and characterization of an extremely thermostable maltogenic amylase, with an ...
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Hyperthermophilic Protein Scaffolds for Engineering Biomolecular Recognition.
... nature, hyperthermophilic archaea and bacteria have evolved to withstand extreme conditions of temperature and ...result, hyperthermophilic proteins are excellent candidates for use as protein scaffolds to ...
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Functional Genomics Investigation of Microbial Physiology in the Hyperthermophilic Microorganisms Pyrococcus furiosus and Thermotoga maritima
... Full genome cDNA microarrays were used to assess differential gene expression patterns in the hyperthermophilic archaeon Pyrococcus furiosus grown on a range of glucose- based carbohydr[r] ...
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Discovery, Functional Genomics and Biochemical Characterization of Alpha-specific Glycosyl Hydrolases from Hyperthermophilic Microorganisms
... In the post-genomics era, functional annotation of biocatalysts encoded in genome sequences represents an important step in biotechnological applications. Despite the large number of sequenced genomes available and ...
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Crystal Structure of NAD dependent Peptoniphilus Asaccharolyticus Glutamate Dehydrogenase Reveals Determinants of Cofactor Specificity
... the hyperthermophilic archaeon Pyrobaculum islandicum (PyrGDH) in complex with NAD + (PDB code 1v9l) was used to generate the approximate position of the cofactor in the bacterial enzymes PaGDH and ...
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N-terminal domain replacement changes an archaeal monoacylglycerol lipase into a triacylglycerol lipase
... a hyperthermophilic archaeon Ther- mococcus onnurineus (strain NA1) and have successfully changed it into a triglyceride lipase through lid engineer- ing ...
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Breath-giving cooperation: critical review of origin of mitochondria hypotheses
... the archaeon based on the required strict anaerobiosis and on the fact that methanogenesis-related genes are absent from eukary- otes and that archaea-related eukaryotic genes do not re- semble those of classical ...
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First Insights into the Entry Process of Hyperthermophilic Archaeal Viruses
... A decisive step in a virus infection cycle is the recognition of a specific receptor present on the host cell surface, subsequently leading to the delivery of the viral genome into the cell interior. Until now, the early ...
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