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Immunoblot assay

Immunoblot assay for serodiagnosis of Helicobacter pylori infections

Immunoblot assay for serodiagnosis of Helicobacter pylori infections

... An immunoblot assay for the serological diagnosis of Helicobacter pylori infection was ...the immunoblot assay. In addition, the Ge-EIA and the immunoblot assay were compared ...

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Immunoblot Assay Using Recombinant Antigens as a Supplemental Test To Confirm the Presence of Antibodies to Trypanosoma cruzi

Immunoblot Assay Using Recombinant Antigens as a Supplemental Test To Confirm the Presence of Antibodies to Trypanosoma cruzi

... the immunoblot assay (three test bands) and were also clearly positive in the other assays, including RIPA, thus sug- gesting that they represent true positives (Table ...disease assay, and one was ...

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Use of Viral Lysate Antigen Combined with Recombinant Protein in Western Immunoblot Assay as Confirmatory Test for Serodiagnosis of Severe Acute Respiratory Syndrome

Use of Viral Lysate Antigen Combined with Recombinant Protein in Western Immunoblot Assay as Confirmatory Test for Serodiagnosis of Severe Acute Respiratory Syndrome

... Western immunoblot assay and analysis. The Western immunoblot assay was carried out at room temperature on a rocking platform for all incubation ...

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Serological Determination of Hepatitis C Virus Subtypes 1a, 1b, 2a, 2b, 3a, and 4a by a Recombinant Immunoblot Assay

Serological Determination of Hepatitis C Virus Subtypes 1a, 1b, 2a, 2b, 3a, and 4a by a Recombinant Immunoblot Assay

... an immunoblot assay for the serological differentiation of HCV genotypes and subtypes on the basis of the use of recombinant proteins derived from the NS-4 region of ...the assay described here is ...

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Detection of Antibodies against Adenovirus Protein IX, Fiber, and Hexon in Human Sera by Immunoblot Assay

Detection of Antibodies against Adenovirus Protein IX, Fiber, and Hexon in Human Sera by Immunoblot Assay

... an immunoblot assay using recombinant pIX and fiber proteins had not been described until ...An immunoblot assay like that described here, which uses the strong immune response to ...

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Improvement of Lyme Borreliosis Serodiagnosis by a Newly Developed Recombinant Immunoglobulin G (IgG) and IgM Line Immunoblot Assay and Addition of VlsE and DbpA Homologues

Improvement of Lyme Borreliosis Serodiagnosis by a Newly Developed Recombinant Immunoglobulin G (IgG) and IgM Line Immunoblot Assay and Addition of VlsE and DbpA Homologues

... line immunoblot assay based on 18 homologues of seven different antigens, ...Western immunoblot assay ...line immunoblot assay offers a useful improvement in serodiagnosis of ...

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Novel Immunoblot Assay Using Four Recombinant Antigens for Diagnosis of Epstein Barr Virus Primary Infection and Reactivation

Novel Immunoblot Assay Using Four Recombinant Antigens for Diagnosis of Epstein Barr Virus Primary Infection and Reactivation

... novel immunoblot assay has diagnostic capabilities comparable to those of the IF assay and therefore provides a new alternative for the screen- ing and diagnosis of EBV ...

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Use of Immunoblot Assay To Define Serum Antibody Patterns Associated with Helicobacter pylori Infection and with H  pylori Related Ulcers

Use of Immunoblot Assay To Define Serum Antibody Patterns Associated with Helicobacter pylori Infection and with H pylori Related Ulcers

... The choice of a single method for the diagnosis of H. pylori infection remains controversial, and at present, two different methods are needed to determine whether a patient is infected (28). The bacteriological method ...

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An Immunoblot Assay for Detection of Immunoglobulin M Antibody to Human Herpesvirus 6

An Immunoblot Assay for Detection of Immunoglobulin M Antibody to Human Herpesvirus 6

... (IgG)-based immunoblot assay (IB) which spe- cifically detects the 101-kDa HHV-6 immunodominant virion protein (101K) and does not react with HHV-7 cross-reactive antibodies known to be present in human ...

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Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM  and IgG Class Antibodies to Borrelia burgdorferi

Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM and IgG Class Antibodies to Borrelia burgdorferi

... microarray immunoblot (MIB) assays which received FDA clearance in ...MIB assay and the p18, p23, p28, p30, p39, p41, p45, p58, p66, and p93 antigens for the ViraChip IgG MIB ...

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Slot immunoblot assay for detection and quantitation of periodontal disease associated microorganisms in dental plaque

Slot immunoblot assay for detection and quantitation of periodontal disease associated microorganisms in dental plaque

... The most commonly used methods of detection and enumeration of these species have been culture and biochemical analyses, which have generally been considered the "gold standard." However[r] ...

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An immunoblot assay reveals that bacteriophage T4 thymidylate synthase and dihydrofolate reductase are not virion proteins.

An immunoblot assay reveals that bacteriophage T4 thymidylate synthase and dihydrofolate reductase are not virion proteins.

... direct assay since we can now synthe- size single virus proteins devoid of cross-reacting virion species and raise antisera against ...the assay in discriminat- ing between structural and nonstructural ...

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Evaluation of a Treponema pallidum western immunoblot assay as a confirmatory test for syphilis

Evaluation of a Treponema pallidum western immunoblot assay as a confirmatory test for syphilis

... The results shown in Table 3 indicate that the Western blot assay and FTA-ABS DS were in close agreement, except for the following discordant results: the Western blot assay detected all[r] ...

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Western blot (immunoblot) assay of small, round structured virus associated with an acute gastroenteritis outbreak in Tokyo

Western blot (immunoblot) assay of small, round structured virus associated with an acute gastroenteritis outbreak in Tokyo

... Such a band was not observed when the strip was treated with control paired serum samples from patients infected with some enteric viruses and enteropathogenic bacteria or when strips wh[r] ...

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Taenia saginata Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis

Taenia saginata Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis

... immunosorbent assay (ELISA) and immunoblot analysis to diagnose human ...By immunoblot assay, the 64- to 68-kDa component from the ConA-unbound fraction showed 100% sensitivity and ...

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Antibodies to 60-Kilodalton Heat Shock Protein and Outer Membrane Protein 2 of Chlamydia pneumoniae in Patients with Coronary Heart Disease

Antibodies to 60-Kilodalton Heat Shock Protein and Outer Membrane Protein 2 of Chlamydia pneumoniae in Patients with Coronary Heart Disease

... immunosorbent assay (ELISA) and immunoblot assay antibody titers to the recombinant antigens in samples from 219 patients with coronary heart disease (CHD), 179 patients with unstable angina (UA), 40 ...

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Cloning and Expression of Immunoreactive Antigens from Mycobacterium tuberculosis

Cloning and Expression of Immunoreactive Antigens from Mycobacterium tuberculosis

... our assay system indicated that the ...test assay was reported to give a high degree of specificity ...the immunoblot assay to decrease ...

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New laboratory guidelines for serologic diagnosis of Lyme disease: evaluation of the two test protocol

New laboratory guidelines for serologic diagnosis of Lyme disease: evaluation of the two test protocol

... negative immunoblot assay result should be interpreted as ...positive immunoblot assay result should be interpreted as ...IgM immunoblot test result is not recommended for use in ...

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Immunoblots for detection of toxic shock syndrome toxin 1 produced by Staphylococcus aureus

Immunoblots for detection of toxic shock syndrome toxin 1 produced by Staphylococcus aureus

... Colony immunoblot assay for the detection of staphylococcal toxic shock syndrome toxin 1 TSST-1 with anti-TSST-1 Fab'2 fragments.. Rapid screening assay for toxic shock syndrome toxin pr[r] ...

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Comparison of a Multiplexed Herpes Simplex Virus Type-Specific Immunoglobulin G Serology Assay to Immunoblot, Western Blot, and Enzyme-Linked Immunosorbent Assays

Comparison of a Multiplexed Herpes Simplex Virus Type-Specific Immunoglobulin G Serology Assay to Immunoblot, Western Blot, and Enzyme-Linked Immunosorbent Assays

... the assay, along with the unavailability of a commercial assay, make it impractical for routine clinical lab- oratory ...The immunoblot assay allows for the determination of antibody responses ...

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