immunofluorescence assay
Surface immunofluorescence assay for diagnosis of Lyme disease
... A surface immunofluorescence assay SIFA was analyzed and compared with a conventional indirect immunofluorescence assay IFA and whole-cell enzyme-linked immunosorbent assay ELISA for det[r] ...
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Connective tissue disorders: Detection of anti-nuclear antibodies and comparative evaluation of enzyme immune assay with indirect immunofluorescence assay
... This is to certify that the dissertation entitled “CONNECTIVE TISSUE DISORDERS. DETECTION OF ANTI-NUCLEAR ANTIBODIES AND COMPARATIVE EVALUATION OF ENZYME IMMUNE ASSAY WITH INDIRECT IMMUNOFLUORESCENCE ...
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Evaluation of Vircell Enzyme-Linked Immunosorbent Assay and Indirect Immunofluorescence Assay for Detection of Antibodies against Legionella pneumophila
... indirect immunofluorescence assay (IFA) for Legionella pneumophila serogroup 1, the IgM and IgG enzyme-linked immunosorbent assay (ELISA) for Legionella pneumophila serogroup 1, and the IgM-plus-IgG ...
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Flow cytometric immunofluorescence assay for detection of antibodies to human immunodeficiency virus type 1 using insoluble precursor forms of recombinant polyproteins as carriers and antigens
... serological assay, the recombinant flow cytometric immunofluorescence assay (r-FIFA), was developed for the early detection of human immunodeficiency virus type 1 (HIV-1) antibodies by using ...
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Comparison of enzyme linked immunosorbent assay, indirect immunofluorescence assay, and virus isolation for detection of respiratory viruses in nasopharyngeal secretions
... The purpose of this study was to simultaneously compare enzyme-linked immunosorbent assay ELISA, immunofluorescence assay IFA, and the standard virus isolation technique VIT for the dete[r] ...
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Sensitive detection of protein biomarkers using silver nanoparticles enhanced immunofluorescence assay
... nanoparticles (AgNPs) are especially attractive due to their unique advantages, such as easy preparation of Ag hybridized composites,[28] catalyzed deposition,[29] and easy conversion to silver ions.[30] Fluorescence ...
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Multiple immunofluorescence assay identifies upregulation of Active β catenin in prostate cancer
... multiple immunofluorescence assay demonstrates that the upregulation of Active β‑catenin is a relatively common feature of prostate tumor development, and further supports the activation of the ...
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Comparison of slide agglutination test and direct immunofluorescence assay for identification of Legionella isolates
... With polyvalent, pooled antisera and absorbed, serogroupspecific antisera, the slide agglutination test is a useful alternative to the direct immunofluorescence assay in the diagnosis of[r] ...
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Treponema pallidum Surface Immunofluorescence Assay for Serologic Diagnosis of Syphilis
... surface immunofluorescence assay (SIFA) using live spirochetes was analyzed and compared with Western blot (WB), fluorescent treponemal antibody absorption (FTA-ABS), microhemagglutination (MHA-TP), and ...
5
Simultaneous Detection and Differentiation of Anti-Helicobacter pylori Antibodies by Flow Microparticle Immunofluorescence Assay
... immunosorbent assay (ELISA), it is desirable to obtain additional information concerning the presence of certain virulence factors of ...microparticle immunofluorescence assay (FMIA) for detection of ...
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Subtyping Influenza A Virus with Monoclonal Antibodies and an Indirect Immunofluorescence Assay
... definitive immunofluorescence assay for the subtyping of influenza A virus isolates, we generated a panel of monoclonal antibodies (MAbs) against the major circulat- ing influenza A virus subtypes using ...
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Evaluation of Four Commercially Available Epstein-Barr Virus Enzyme Immunoassays with an Immunofluorescence Assay as the Reference Method
... indirect immunofluorescence assay as the reference method, for Epstein-Barr virus (EBV) VCA (viral capsid antigen) immunoglobulin G (IgG), VCA IgM, or EBNA (EBV nuclear antigen) IgG at three different ...
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New Criteria for Immunofluorescence Assay for Q Fever Diagnosis in Japan
... specific protein by WBA, although a few of them had a signif- icant index by ELISA (Table 4). This is in accordance with a previous study showing that ELISA is suitable for use as a screening assay for Q fever ...
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Serological detection of Helicobacter pylori by a flow microsphere immunofluorescence assay
... Sera from the same 20 patients were assayed by FMIA using microspheres coated with each of the three antigen preparations, and WC and PEMS gave results comparable to those of ELISAs usin[r] ...
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Comparison of cytomegalovirus antigenemia assay with shell vial indirect immunofluorescence assay for rapid detection of cytomegalovirus in blood
... Cytomegalovirus CMV antigenemia assay is more sensitive than shell vial cultures for rapid detection of CMV in polymorphonuclear blood leukocytes.. Monitoring of human cytomegalovirus in[r] ...
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Diagnosis of spirochetal meningitis by enzyme linked immunosorbent assay and indirect immunofluorescence assay in serum and cerebrospinal fluid
... The highest diagnostic sensitivity, 91 %, was demonstrated by measurement of CSF antibodies and calculation of a spirochetal CSF titer index, which is the ratio of ELISA titer in CSF/ELI[r] ...
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Evaluation of an Indirect Immunofluorescence Assay for Strongyloidiasis as a Tool for Diagnosis and Follow-Up
... Strongyloidiasis is a chronic, soil-transmitted helminthiasis with a worldwide distribution. Current estimates indicate that it affects 30 to 100 million people, but accurate prevalence data are lacking. Although ...
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Validation of Legionella pneumophila indirect immunofluorescence assay with epidemic sera
... The sensitivity of the IFA test as currently used by CDC was estimated to be at least that of the original test, on the basis of a comparison of titers against the Philadelphia 1 antigen[r] ...
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Enhanced uptake, high selective and microtubule disrupting activity of carbohydrate fused pyrano pyranones derived from natural coumarins attributes to its anti malarial potential
... by immunofluorescence assay indicates that carbohybrid 12 disrupts microtu- bule dynamics of parasite giving diffuse staining in late stage trophozoites and schizonts after 24 h of treatment in ring and ...
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Simple diagnosis of Encephalitozoon sp microsporidial infections by using a panspecific antiexospore monoclonal antibody
... by immunofluorescence of all known Encephalitozoon spores affecting ...immunosorbent assay, immunofluorescence, Western blot, and immunoelectron microscopy using Encephalitozoon species from fresh ...
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