Immunofluorescence microscopy
Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
... electron microscopy and confocal immunofluorescence microscopy have both shown that LDs are localized in close proximity to the mitochondrial network in skeletal muscle (Hoppeler 1999; Shaw et ...
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Immunofluorescence Microscopy and Flow Cytometry Characterization of Chemical Induction of Latent Epstein-Barr Virus
... by immunofluorescence micros- copy (20), a time-consuming and labor-intensive method that requires much experience for accurate interpretation of re- ...conventional immunofluorescence microscopy. In ...
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Analyzing pathogenic (double stranded (ds) DNA specific) plasma cells via immunofluorescence microscopy
... Methods: With improved fluorochrome labeling of dsDNA, removal of DNA aggregates, and enhanced blocking of unspecific binding, we were able to specifically detect dsDNA-reactive plasma cells by immunofluorescence ...
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Characterization of a panel of six β2-adrenergic receptor antibodies by indirect immunofluorescence microscopy
... A similar protocol was used for localization of the endog- enous β 2 AR in primary cultures of rat airway smooth mus- cle cells (ASMC) and rat airway epithelial cells (AEC) except that the samples were double-labeled ...
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Rapid detection and identification of JC virus and BK virus in human urine by using immunofluorescence microscopy
... Polyoma virus particles were typically identified by electron microscopy in urine in which abnormal cells and nuclear antigen were repeatedly seen Table 1.. A prolonged search of several[r] ...
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Cultured human amniotic fluid cells characterized with antibodies against intermediate filaments in indirect immunofluorescence microscopy
... FIGURE 5 Fibroblastic cells, characterized by their typical fibrillar cytoplasmic staining only with vimentin antibodies in IIF microscopy a, b were only occasionally seen in normal huma[r] ...
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Uukuniemi virus maturation: immunofluorescence microscopy with monoclonal glycoprotein-specific antibodies.
... Double-staining experiments with monoclonal antibodies which preferentially stained the Golgi-associated viral glycoproteins and with anti-N polyclonal rabbit antiserum showed a correlat[r] ...
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Antibodies to neurofilament protein in retinitis pigmentosa
... Abstract Antibodies reactive with heterologous neural tissue were detected by indirect immunofluorescence microscopy in the sera of 17 of 34 patients with retinitis pigmentosa, one of 30[r] ...
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Intermediate filaments in malignant melanomas Identification and use as marker in surgical pathology
... Intermediate-sized filaments have been studied in human malignant melanomas and in normal melanocytes by immunofluorescence microscopy with antibodies directed against keratin, vimentin, desmin, ...
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Validation of various parasite detection tests for use in the Australian marsupials quenda (Isoodon obesulus) and brushtail possums (Trichosurus vulpecula)
... detecting Toxoplasma gondii infection in quenda ( Isoodon obesulus ) and brushtail possums 17.. ( Trichosurus vulpecula ); 2) immunofluorescence microscopy of feces and a PCR and sequ[r] ...
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Exposure of the Plasmodium falciparum clonally variant STEVOR proteins on the merozoite surface
... indirect immunofluorescence microscopy, parasites were cultured and synchronized as described above and allowed to grow for one cycle before samples were pro- ...
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Biogenesis of type I cytopathic vacuoles in Semliki Forest virus-infected BHK cells.
... By using monospecific anti-nsP3 antibodies in combination with immunofluorescence microscopy, we showed recently that SFV-specific nonstructural protein nsP3 is localized to large cytopl[r] ...
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Generation of Filamentous Instead of Icosahedral Particles by Repression of African Swine Fever Virus Structural Protein pB438L
... Electron microscopy of vB438Li-infected cells. To establish more precisely the step at which virus morphogenesis is blocked in the absence of protein pB438L, cells infected with vB438Li virus in the presence or in ...
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Luibl, Volker (2006): Association of nonfribrillar amyloid oligomers with drusen of aging and age-related macular degeneration. Dissertation, LMU München: Tierärztliche Fakultät
... Furthermore, immunofluorescence microscopy demonstrates oligomers in vesicular form or bound to vesicles in ...(electron microscopy result), a size which has been also observed of „prion-exosomes“ ...
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Maintenance of active chromatin states by HMGN2 is required for stem cell identity in a pluripotent stem cell model
... by immunofluorescence and ...2b). Immunofluorescence microscopy revealed that Nanog protein expression is reduced in most cells in all the Hmgn1 and Hmgn2-knockout cul- tures, even in the N1-a and ...
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The Human Papillomavirus Type 16 E5 Oncoprotein Translocates Calpactin I to the Perinuclear Region
... used immunofluorescence microscopy to investigate whether the association of calpactin I with E5 alters the localization of ANXA2 and p11 subunits in stable cell lines expressing epitope-tagged E5, E5( ⫺ ...
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Real Time PCR for detection of herpes simplex virus without nucleic acid extraction
... Background: The speed and sensitivity of real-time polymerase chain reaction (PCR) have made it a popular method for the detection of microbiological agents in both research and clinical specimens. For the detection and ...
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Mutational analyses of the intergenic dinucleotide and the transcriptional start sequence of vesicular stomatitis virus (VSV) define sequences required for efficient termination and initiation of VSV transcripts.
... used immunofluorescence microscopy to deter- mine if any of the nucleotide changes affected M and/or G protein expression ...by immunofluorescence microscopy, was almost completely eliminated ...
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Human Rhinovirus 14 Enters Rhabdomyosarcoma Cells Expressing ICAM-1 by a Clathrin-, Caveolin-, and Flotillin-Independent Pathway
... The immunofluorescence results were further supported by using pharmacological ...by immunofluorescence microscopy which revealed HRV14 at or close to the membrane in the presence of either ...
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Initiation of Hepatitis Delta Virus Genome Replication
... molecular-weight ribonucleoprotein (RNP) structures. After transfection of these RNPs into human cells, we detected HDV genome replication, as assayed by Northern analysis or immunofluorescence microscopy. ...
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