In planta
Hyperthermophilic endoglucanase for in planta lignocellulose conversion
... The use of plants as an enzyme production system and subsequent utilization requires two important criteria to be satisfied. The enzyme must not interfere with cell wall synthesis or integrity and it must tolerate the ...
9
Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry
... in planta BiFC screen provides a lucrative alter- native to search for novel protein-protein interactions that can, according to our data, only be found in ...
17
The In Planta Transcriptome of Ralstonia solanacearum: Conserved Physiological and Virulence Strategies during Bacterial Wilt of Tomato
... in planta transcriptome included genes encoding transporters of anions, tricarboxylic acid (TCA) cycle intermediates, and amino acids known to be present in to- mato xylem fluid ...in planta, although ...
11
Potyviral VPg Enhances Viral RNA Translation and Inhibits Reporter mRNA Translation In Planta
... Next, the effect of mutant VPgs on reporter protein expres- sion was studied. VPg expression constructs or the 35S-fluc-nos construct was preinfiltrated into the plants. On the next day, the 35S-rluc-nos construct was ...
12
Complete Genome Sequence and In Planta Subcellular Localization of Maize Fine Streak Virus Proteins
... in planta localization results suggest that ORF2 of MFSV encodes the P protein because (i) P is located adjacent to N in all rhabdoviruses sequenced so far, (ii) the predicted size of the ORF2 protein is similar ...
11
The Maternal Chromosome Set Is the Target of the T-DNA in the in Planta Transformation of Arabidopsis thaliana
... In planta transformation methods are now commonly used to transform Arabidopsis thaliana by Agrobacter- ium tumefaciens. The origin of transformants obtained by these methods has been studied by inoculating ...
13
In planta assays involving epigenetically silenced genes reveal inhibition of cytosine methylation by genistein
... during the meiosis and is not transmitted to the subse- quent generation. As a consequence, the progeny plants that were used for the assay in the present study do not have viral RNAs that trigger de novo methylation on ...
10
Effect of chemical treatment as a guide for in planta acetylation of starch required for the production of biodegradable plastics
... in planta acetylation researches should be prioritized in a bid to modify starch in situ in order to forestall the unforeseen problem of chemical pollution of the ...
7
In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize
... E1-expressing tobacco plants were pretreated in 1% (wt/vol) sulfuric acid for 10 minutes at three different temperatures, 110°C, 140°C and 170°C, resulting in a range of severities. Figure 3 illustrates that at 110°C and ...
11
Mutations in the Capsid Protein of Brome Mosaic Virus Affecting Encapsidation Eliminate Vesicle Induction In Planta: Implications for Virus Cell-to-Cell Spread
... Positive-strand RNA viruses are known to rearrange the endomembrane network to make it more conducive for replication, maturation, or egress. Our previous transmission electron microscopic (TEM) analysis showed that ...
11
The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods
... Based on its designation one might expect that BiFC pro- vides a direct measure for bimolecular interactions. How- ever, as for all FCAs that are carried out in vivo, it is possible that a third protein mediates the ...
14
The application of an Agrobacterium -mediated in planta transformation system in a Catharanthus roseus medicinal plant
... in planta transformation method is a tissue culture independent strategy for the transgenic line production in which the plantlets are infected by Agrobacterium and are generated free of laborious and ...
8
Tissue Culture-based Agrobacterium -mediated and in planta Transformation Methods
... in planta gene transformation methods, in addition to the removal of tissue culture optimization, some parts of gene transformation optimization such as antibiotics usage to kill Agrobacterium and to select ...
11
In planta transient expression as a system for genetic and biochemical analyses of chlorophyll biosynthesis
... in planta transient expression has been the characterization of signaling events involved in the initiation of a type of programmed cell death termed the hypersensitive response that is asso- ciated with pathogen ...
10
Standarizing in Planta Agrobacterium Tumefacience Mediated Genetic Transformation Protocol to Develop New Events by Transforming G. Hirsutum Cotton based on Cry1Ac-Cry1Ec Genes
... Abstract: Cotton breeding for insect resistance has been limited by a lack of sufficient genetic variation in the existing germplasms. Therefore, genetic engineering provides the possibility of creating varieties ...
10
SHORT COMMUNICATION AGROBACTERIUM MEDIATED IN PLANTA TRANSFORMATION OF GOSSYPIUM HIRSUTUM CV. G. COT. 10
... An improved protocol of using simple in planta transformation methods (Injection method and Cut method) were developed for Gossypium hirsutum. The frequency of transformation was 1.16 % for injection method and ...
6
Conserved Domains Required for DEMETER 5-Methylcytosine DNA Glycosylase/lyase Function in planta.
... The dme-2 induced CG hypermethylation at selected maternal expressed genes ( SDC , FWA and DRB2 ) and paternally expressed gene ( YUC10 ) is reduced in h1 xLer endosperm, althoug[r] ...
160
In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA
... In order to investigate whether the gus gene silencing in fungal cells would be associated to Figure 4 Presence and expression of the gus gene in transformed Fusarium verticillioides iso[r] ...
11
Overexpression of a Novel Biotrophy-Specific Colletotrichum truncatum Effector, CtNUDIX, in Hemibiotrophic Fungal Phytopathogens Causes Incompatibility with Their Host Plants
... in planta late biotrophic phase of the ...in planta fungal ...in planta biotrophic and necrotrophic phases of ...in planta biomass of ...
10
The C2 domain protein QUIRKY and the receptor like kinase STRUBBELIG localize to plasmodesmata and mediate tissue morphogenesis in Arabidopsis thaliana
... To investigate whether SUB:EGFP and mCherry:QKY can interact physically in planta, we performed Förster Resonance Energy Transfer – Fluorescence Lifetime Imaging Microscopy FRETFLIM assa[r] ...
10