in vitro-matured oocytes
Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation
... in vitro matured and vitrified oocytes, it is reasonable to suggest this innovative and non-invasive alternative, under institutional review board ...in vitro matured oocytes may ...
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Original Article Vitrification of in vitro matured oocytes: effects on meiotic spindle configuration and mitochondrial function
... in vitro-matured oocytes is a new strategy to extend the pool of total ...immature oocytes were donated from women undergoing ICSI ...MII oocytes from vIVM group sharply decreased, ...
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Nuclear remodelling and early development in cryopreserved, porcine primordial germ cells following nuclear transfer into in vitro matured oocytes
... Int J De,' Bini 39 6 19 644 (1995) O,igilla[ A, 'ide Nuclear remodelling and early development in cryopreserved, porcine primordial germ cells following nuclear transfer into in vitro matured oocytes[.] ...
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Application of a zona pellucida binding assay (ZBA) in the domestic cat benefits from the use of in vitro matured oocytes
... The ability of cat spermatozoa to capacitate and acrosome react is essential for a successful zona penetration but not for zona binding [9,14] and corresponding results have been found in the female dog [8,16,17]. When ...
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Niacin improves maturation and cryo-tolerance of bovine in vitro matured oocytes: An experimental study
... in oocytes and, consequently, improve tolerance to ...IVM oocytes after freezing (16). Since vitrification increases ROS in vitro, oxidative stress causes damage to macromolecules, including lipids, ...
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The Impact of In vitro Oocyte Maturity on Developmental Potential of Embryos Derived from Controlled Ovarian Stimulation Cycles
... Even with some differences in the study design, sample size, infertility etiology and stimulation protocols, similar results were reported by authors. Regarding the fertilization rates, our results are similar to the ...
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Production of Boer goat ( Capra hircus ) by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes
... in vitro matured oocytes of Huanghuai white goat, an Anhui native goat ...in vitro and then the morphologically normal embryos were transferred to the ...
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Demant, Myriam (2012): Qualitative and quantitative proteome analyses of bovine oocytes and early embryos. Dissertation, LMU München: Tierärztliche Fakultät
... All oocytes were aspired in vivo in so called “OPU ...performed. Oocytes were either aspired from unstimulated cows in the GV oocyte stage or from FSH stimulated cows in the MII oocyte ...GV oocytes ...
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The secretions of oviduct epithelial cells increase the equine in vitrofertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?
... In the equine, numerous attempts to establish an efficient IVF technique were performed during the last decades [24- 27]. However, reported fertilization rates range from 0% to 60% which are lower than the IVF rates of ...
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An efficient method for the sanitary vitrification of bovine oocytes in straws
... in vitro matured oocytes were exposed to 20% ethylene glycol (EG20) for 5 min and 40% ethylene glycol (EG40) for 30 s, followed by treatment with 30% glycerol (Gly30), Gly40 or Gly50, a volume ...
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Effects of Vitrification on Immature and in vitro Matured, Denuded and Cumulus Compact Goat Oocytes and their Subsequent Fertilization
... of oocytes and the presence or absence of cumulus cells (10, 11, 16, ...stage oocytes tolerate the cryopreservation damage more efficiently compared to oocytes at meta- phase-II and cumulus compact ...
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Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide donor
... in vitro matured oocytes did not probably influence the resulting rate of parthenogenetic ...of oocytes reach- ing the stage of metaphase II triggers oocyte ageing and this is accompanied by ...
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The role of nitric oxide synthase isoforms in aged porcine oocytes
... porcine oocytes was ...in oocytes were relatively low and eNOS indicated a higher-intensity signal, the differences in the signal decrease rate may have resulted from diverse levels of specific isoforms ...
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A study on the use of unfrozen, diluted semen for the in vitro fertilisation of bovine oocytes matured in vitro : a thesis presented in partial fulfilment of the requirements for the degree of Master of Agricultural Science in Animal Science at Massey University
... In experiment 1, semen from each of two bulls was used on two consecutive days "day-old" and "two-day-old" sperm to explore the effect of sperm concentration on oocyte penetration rates.[r] ...
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In vitro aging of porcine oocytes
... aged oocytes spontaneously activated in vitro is generally low (with maxima up to 45%) and is lower than the activation rate a�er the artificial parthenogenesis with various artificial stimuli in pig ...
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Original Article Effects of growth hormone supplementation in poor responders undergoing in vitro fertilization/intracytoplasmic sperm injection treatment
... retrieved oocytes and with subsequent three or less obtained embryos using GnRH agonist long pro- tocol), (3) Antral follicle count (AFC) ≤ 7, and/or basal follicle stimulating hormone (FSH) ≥ ...
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Regulator of G protein signaling 2 (RGS2) suppresses premature calcium release in mouse eggs
... GV oocytes following microinjection with cRNA encoding HA-tagged RGS2, probed with monoclonal anti-RGS2 ...20 oocytes/lane. (B) Immunoblot of RGS2 in control MII eggs or eggs matured to MII following ...
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MicroRNA-21 and PDCD4 expression during in vitro oocyte maturation in pigs
... in vitro maturation of pig ...in vitro maturation, it is of interest if MIR21 expres- sion in the pig cumulus oocyte complex is also responsive to the ...in vitro maturation was ...in vitro ...
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Osmotic-shock produced by vitrification solutions improves immature human oocytes in vitro maturation
... in vitro culture from GV to MII stage determines this ...in vitro aging due to in vitro culture from GV to MII stage determines this ...vitrified oocytes in the MII stage (G3), a high AR and ...
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In vitroeffects of relaxin on gene expression in porcine cumulus-oocyte complexes and developing embryos
... in oocytes matured in the presence of porcine follicular fluid ...study, oocytes were matured in the absence of follicular fluid, and the supplementation of the basal medium with relaxin ...
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