indirect immunofluorescence assay
An indirect immunofluorescence assay using a cell culture derived antigen for detection of antibodies to the agent of human granulocytic ehrlichiosis
... An indirect immunofluorescence assay for the detection of human antibodies to the agent of human granulocytic ehrlichiosis (HGE) was developed and ...The assay readily detected specific ...
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Detection of Epstein-Barr virus infection subtype in patients with multiple sclerosis by indirect immunofluorescence assay
... immunosorbent assay (ELISA) to confirm the relationship between serum/cerebrospinal fluid (CSF) EBV antigen-antibody and the occurrence of ...contrast, indirect immunofluorescence assay (IFA) ...
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Subtyping Influenza A Virus with Monoclonal Antibodies and an Indirect Immunofluorescence Assay
... definitive immunofluorescence assay for the subtyping of influenza A virus isolates, we generated a panel of monoclonal antibodies (MAbs) against the major circulat- ing influenza A virus subtypes using ...
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Diagnostic value of the indirect immunofluorescence assay in cat scratch disease with Bartonella henselae and Afipia felis antigens.
... with B. henselae DNA) in lymph nodes of some patients suf- fering from CSD. Therefore, it appeared again that A. felis could be responsible for CSD, at least in particular epidemio- logical circumstances or in particular ...
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Connective tissue disorders: Detection of anti-nuclear antibodies and comparative evaluation of enzyme immune assay with indirect immunofluorescence assay
... This is to certify that the dissertation entitled “CONNECTIVE TISSUE DISORDERS. DETECTION OF ANTI-NUCLEAR ANTIBODIES AND COMPARATIVE EVALUATION OF ENZYME IMMUNE ASSAY WITH INDIRECT IMMUNOFLUORESCENCE ...
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Evaluation of Vircell Enzyme-Linked Immunosorbent Assay and Indirect Immunofluorescence Assay for Detection of Antibodies against Legionella pneumophila
... IgM indirect immunofluorescence assay (IFA) for Legionella pneumophila serogroup 1, the IgM and IgG enzyme-linked immunosorbent assay (ELISA) for Legionella pneumophila serogroup 1, and the ...
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Comparison of Enzyme-Linked Immunosorbent Assay, Western Blotting, Microagglutination, Indirect Immunofluorescence Assay, and Flow Cytometry for Serological Diagnosis of Tularemia
... immunosorbent assay (ELISA), microag- glutination (MA), Western blotting (WB), an indirect immunofluorescence assay (IIFA), and flow cytometry ...
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Comparison of enzyme linked immunosorbent assay, indirect immunofluorescence assay, and virus isolation for detection of respiratory viruses in nasopharyngeal secretions
... The purpose of this study was to simultaneously compare enzyme-linked immunosorbent assay ELISA, immunofluorescence assay IFA, and the standard virus isolation technique VIT for the dete[r] ...
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Typing of herpes simplex virus with type specific human immunoglobulin M in an indirect immunofluorescence assay
... Fixed cells infected with the McIntyre strain and cells infected with MS were overlaid with serial dilutions of the test serum, reference serum containing IgG reactive with both HSV-1 an[r] ...
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Validation of Legionella pneumophila indirect immunofluorescence assay with epidemic sera
... The sensitivity of the IFA test as currently used by CDC was estimated to be at least that of the original test, on the basis of a comparison of titers against the Philadelphia 1 antigen[r] ...
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Evaluation of an Indirect Immunofluorescence Assay for Strongyloidiasis as a Tool for Diagnosis and Follow-Up
... Strongyloidiasis is a chronic, soil-transmitted helminthiasis with a worldwide distribution. Current estimates indicate that it affects 30 to 100 million people, but accurate prevalence data are lacking. Although ...
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Comparison of cytomegalovirus antigenemia assay with shell vial indirect immunofluorescence assay for rapid detection of cytomegalovirus in blood
... Cytomegalovirus CMV antigenemia assay is more sensitive than shell vial cultures for rapid detection of CMV in polymorphonuclear blood leukocytes.. Monitoring of human cytomegalovirus in[r] ...
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Diagnosis of spirochetal meningitis by enzyme linked immunosorbent assay and indirect immunofluorescence assay in serum and cerebrospinal fluid
... The highest diagnostic sensitivity, 91 %, was demonstrated by measurement of CSF antibodies and calculation of a spirochetal CSF titer index, which is the ratio of ELISA titer in CSF/ELI[r] ...
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Detection of Specific Autoantibodies in Sera with Negative Antinuclear Antibody by Indirect Immunofluorescence Assay but Positive by Enzyme Immunoassay
... The manufacturer’s recommended cut-off value yielded a low PPV. Even though the EIA value was as high as 250 U/mL, the PPV of 75% was not sufficiently high. Therefore, a positive result from this EIA kit may not be ...
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Serodiagnosis of Louse Borne Relapsing Fever with Glycerophosphodiester Phosphodiesterase (GlpQ) fromBorrelia recurrentis
... an indirect immunofluorescence assay (IFA) and an enzyme-linked immunosorbent assay (ELISA) that used whole cells of ...the assay was more sensitive than the whole-cell IFA and ELISA ...
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Childhood nosocomial viral acute respiratory tract infections in teaching hospital Anuradhapura, Sri Lanka
... Abbreviations HA: hospital-acquired; CA: community-acquired; ARTI: viral acute respiratory tract infections; IFA: indirect immunofluorescence assay; HAI: hospital-acquired infections; NP[r] ...
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Simultaneous presence of different Borrelia burgdorferi genospecies in biological fluids of Lyme disease patients
... molecular assay ca- pable of targeting genospecies-specific ospA ...bent assay [ELISA] and indirect immunofluorescence assay) (5) currently used for diagnosis are not capable of ...
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Surface immunofluorescence assay for diagnosis of Lyme disease
... A surface immunofluorescence assay SIFA was analyzed and compared with a conventional indirect immunofluorescence assay IFA and whole-cell enzyme-linked immunosorbent assay ELISA for det[r] ...
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Monoclonal antibody recognizing a species specific protein from Streptococcus pneumoniae
... However, MAbs to the 37-kDa antigen bound whole cells in the enzyme-linked immunosorbent assay and the indirect immunofluorescence assay.. Antibody-binding epitopes of the antigen are pr[r] ...
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Isolation of H9N2 Subtype of Avian Influenza Viruses during an Outbreak in Chickens in Iran
... Avian influenza is an important disease of poultry with the potential to cause major epidemics resulting in significant economic losses. The presence of avian influenza viruses (AIV) in chickens in Iran has not been ...
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