Indoleamine 3,5-dioxygenase
![Synthesis of 5-[18F]Fluoro-α-methyl Tryptophan: New Trp Based PET Agents](data:image/gif;base64,R0lGODlhAQABAIAAAP///wAAACH5BAEAAAAALAAAAAABAAEAAAICRAEAOw==)
Synthesis of 5-[18F]Fluoro-α-methyl Tryptophan: New Trp Based PET Agents
... Indoleamine 2,3-dioxygenase (IDO1) plays a special role in the biology of various cancer types, because it breaks down the essential amino acid tryptophan for immune cell activation. Upregulation of IDO1 ...
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Brain indoleamine 2,3 dioxygenase contributes to the comorbidity of pain and depression
... with 5% normal donkey serum in ...PBS 3 times and incubated with 1:300 FITC- or cyanine 3–conjugated secondary antibody (Jackson ImmunoResearch Laborato- ries ...
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H2S suppresses indoleamine 2, 3-dioxygenase 1 and exhibits immunotherapeutic efficacy in murine hepatocellular carcinoma
... MDSCs [4, 35]. Herein, CD11b + myeloid cells were found to aggregate in IDO1 high-expressed (IDO1-high) but not in IDO1 low-expressed (IDO1-low) T- tissues, and CD11b + myeloid cell number was positively correlated with ...
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Inhibition of experimental asthma by indoleamine 2,3 dioxygenase
... IDO controls the pulmonary survival of an OVA-specific Th1 cell line but not of B cells in vivo. IDO activity has already been demonstrated to inhibit activated Th1 cells via the induction of apoptosis (14, 25). ...
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Original Article Expression and function analysis of indoleamine 2 and 3-dioxygenase in bladder urothelial carcinoma
... Bladder urothelial carcinoma (BUC) is one of the most common malignancies in the urinary system and accounts for approximately 15,000 deaths annually in the United States [1]. Most cases of BUC are superficial bladder ...
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Effects of pentoxifylline, 7-nitroindazole, and imipramine on tumor necrosis factor-α and indoleamine 2,3-dioxygenase enzyme activity in the hippocampus and frontal cortex of chronic mild-stress-exposed rats
... Chronic treatment with pentoxifylline or 7-nitroindazole displayed antidepressant-like activity (reversed anhedonia in SPT) that was comparable to imipramine effect. Moreover, early reversal of CMS-induced anhedonia was ...
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Lavender oil suppresses indoleamine 2,3 dioxygenase activity in human PBMC
... During the cellular immune response, in parallel to IDO, guanosine triphosphate (GTP)-cyclohydrolase-I (GTP- CH-I, EC 3.5.4.16) is induced by IFN-γ. GTP-CH-I is the key enzyme in the biosynthesis of neopterin, a marker ...
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Central Administration of Lipopolysaccharide Induces Depressive-like Behavior in Vivo and Activates Brain Indoleamine 2,3 Dioxygenase In Murine Organotypic Hippocampal Slice Cultures
... Hippocampal slice cultures were prepared using the sta- tic interface culture method [39]. Briefly, 6- to 8-day-old C57BL/6J mice were euthanized by decapitation. The brains and meninges were removed, followed by separa- ...
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Regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells
... for 5 minutes on ice and sonicated for 10–15 ...for 5 minutes at 90 ° C, micro-centrifuged briefly, and stored at - 20 ° ...containing 5% BSA was added to the mem- branes and left incubating ...
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Serum Indoleamine 2,3-Dioxygenase Activity Predicts Prognosis of Pulmonary Tuberculosis
... were then spiked with standards and deproteinized with 0.5 N perchloric acid for 10 min on ice. Then, the samples were centrifuged (15,000 ⫻ g; 10 min), and the supernatants were vortex mixed with an equal volume of 1 M ...
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Mesenchymal stem cells overexpressing IL-35: a novel immunosuppressive strategy and therapeutic target for inducing transplant tolerance
... In 2002, Bartholomew et al. [36] observed that MSCs could suppress lymphocyte proliferation in vitro and in vivo. Administration of MSCs led to prolonged skin graft survival in baboons when compared to control animals ...
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B7 costimulation and intracellular indoleamine 2,3 dioxygenase (IDO) expression in peripheral blood of healthy pregnant and non pregnant women
... Calibrants were prepared at 6 different concentration levels, from 1 to 100 nM, 0.1 to 5 μM, 5 to 50 μM and 0.5 to 7.5 μM for KYNA, KYN, TRP and the internal standard, re- spectively. Three parallel ...
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Original Article Overexpressed indoleamine 2, 3-dioxygenase induced T cell apoptosis via Fas/FasL and Bcl-2/Bax-related apoptotic pathways in metastatic breast cancer
... of the Bcl-2 gene family play a pivotal role in controlling the execution machinery of apopto- sis through the mitochondrial pathway. Kluck et al [24] revealed that Bcl-2 inhibited cyto- chrome c translocation, thereby ...
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Expression and regulation of immune modulatory enzyme Indoleamine 2,3 dioxygenase (IDO) by human airway epithelial cells and its effect on T cell activation
... Cells were washed with ice-cold PBS and then lysed using ice-cold radio-immunoprecipitation assay lysis buffer with protease and phosphatase inhibitor (Sigma-Aldrich, St. Louis, MO, USA) on a shaker for 30 min at 4°C. ...
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Decreased protein nitration in macrophages that overexpress indoleamine 2, 3-dioxygenase
... pcDNA3-IDO or Pvu 1-linearized pcDNA-3 vector DNA. We immediately seeded the electroporated cells into tissue culture flasks containing culture media (see above). Before beginning clonal dilution, the ...
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Expression and regulation of immune-modulatory enzyme indoleamine 2,3-dioxygenase (IDO) by human airway epithelial cells and its effect on T cell activation
... a 24-well plate supplemented with 100 μM l-TRP (Sigma-Aldrich, St. Louis, MO, USA), and grown to confluence for 24 h. Cells were stimulated with various TLR agonists and allergen extracts, including LPS and ...
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Emerging therapeutic roles for NAD+ metabolism in mitochondrial and age-related disorders
... IDO: indoleamine 2,3-dioxygenase; TDO: tryptophan 2,3-dioxygenase; ACMS: α-amino-β-carboxymuconate-ε- semialdehyde; QPRT: quinolinate phosphoribosyl transferase; NAMN: nicotinic acid mononucleotide; ...
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Deficiency of immunoregulatory indoleamine 2,3-dioxygenase 1 in juvenile diabetes
... Subject recruitment. Peripheral venous blood (3−5 ml) was obtained from T1D patients of European descent and age-matched children (i.e., those without T1D or any autoimmune or allergic disease) attending ...
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Upregulation of Indoleamine 2,3-Dioxygenase in Hepatitis C Virus Infection
... There is an intricate cross talk between IDO and CTLA-4 (17). It has been shown that tryptophan depletion together with the presence of kynurenines promotes the expression of inhibitory molecules, such as CTLA-4 and ...
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Nanomedicine and cancer immunotherapy: focus on indoleamine 2,3-dioxygenase inhibitors
... the relationship between anti-inflammatory properties of natural products and IDO activity. High concentration of numerous phytochemicals, possessing flavonoid chemical structure, has been reported in various medicinal ...
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