Inducible promoter
Radiation inducible promoter mediated PUMA Gene in the the treatment of Tca8113
... Objective: The aim of this study is to explore the therapeutic effect of the PUMA gene mediated by ra- diation-inducible promoters in the treatment of ton- gue squamous cell carcinoma. Methods: Recombi- nant ...
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Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum
... Several inducible promoters for ...well-established inducible promoter system and is based on the induction of promoters from Lactobacillus sakei with an inducing peptide ...another inducible ...
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Evaluation of the Water Stress-Inducible Promoter Wsi18 in the Model Monocot Brachypodium distachyon
... Glucuronidase has a half life of ~50 hours in plant cells, and so would still be active by the time observations were made (Jefferson et al., 1987). The overnight incubation could have given enough time for low levels of ...
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Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat
... gene promoter can be induced by heat treatment of 38–40 C for 1–2 h in transgenic ...heat-inducible promoter provides the ability to investigate the function of candidate genes by overexpression or ...
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New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica
... EYK1 promoter region using the intergenic region between YALI0F01628g and YALI0F01606g ...EYK1 promoter did not evidenced any conserved motif within Yarrowia lipolytica genome (data not ...the ...
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Identification of a Copper-Inducible Promoter for Use in Ectopic Expression in the Fungal Pathogen Histoplasma capsulatum
... CRP1 promoter as a molecular genetic tool, it is interesting to speculate on whether the CRP1 gene or other CuSO 4 -regulated genes might promote the virulence of ...
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Distinct gene expression signatures induced by viral transactivators of different HTLV 1 subgroups that confer a different risk of HAM/TSP
... an inducible promoter revealed different target gene profiles; (2) the number of differentially regulated genes induced by HBZ was 2–3 times higher than that induced by Tax; (3) Tax and HBZ induced the ...
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Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica
... few inducible promoters are available for algal ...(NR) promoter, which enables expression in the presence of nitrate or absence of nitrogen and repres- sion by ammonium, is the best available ...
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Construction and characterization of a synthetic Baculovirus-inducible 39K promoter
... K promoter is mainly expressed by immediate early genes ...K promoter as induced by different early transcriptional activator ...K promoter (Fig. 3) [21, 38, 39]. The promoter-specific ...
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The UL5 gene of herpes simplex virus type 1: isolation of a lacZ insertion mutant and association of the UL5 gene product with other members of the helicase-primase complex.
... In this study, we isolated a permissive cell line L2-5 which contains the wild-type UL5 gene under the control of the strong and inducible promoter for the large subunit of herpes simple[r] ...
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A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris
... constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; P GAP ) or an inducible promoter ...(e.g. promoter of alcohol oxidase 1; P AOX1 ). Both promoter ...
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Development of a strictly regulated xylose-induced expression system in Streptomyces
... (xylA) promoter and xylose (xyl) operon were chosen in an initial attempt to investigate induced protein expression in a Streptomyces strain using various carbon ...xylA promoter is a useful candidate for ...
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Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production
... the inducible XylS/Pm regulator/promoter system origi- nating from Pseudomonas putida TOL plasmid pWW0 to control production of a model protein, ...PrpsJ promoter of the rpsJ ribosomal protein gene ...
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Heat Induced Reporter and Therapeutic Gene Expression in Cancer Gene Therapy
... collected and counted in a gamma counter. Tumors were also disaggregated in lysis buffer using a Mini-Beadbeater, and murine IL-12 concentrations in tumor extracts were determined using a mIL-12 ELISA kit. In the in ...
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Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter
... p21 promoter falls into this second class of Brn-3a inducible promoters since we were not able to eliminate Brn-3a inducibility without completely inactivating the ...p21 promoter appears to be ...
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T cell activation promotes tumorigenesis in inflammation associated cancer
... Although alternative Tax transgenic models have been described, only two other models were characterized by enhanced T- cell proliferation [38-40]. The role of inflam- mation in those model systems remains to be ...
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Natural history of the ERVWE1 endogenous retroviral locus
... retroviral promoter, the ERVWE1 5' LTR, and (ii) a 436 bp upstream regulatory element (URE), encompassing the MaLR-e1 5' part, that contains the trophoblast specific enhancer (TSE) cited above, conferring high ...
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Controlling heterologous gene expression in yeast cell factories on different carbon substrates and across the diauxic shift: a comparison of yeast promoter activities
... The carbon source has a significant effect on global regulatory patterns. The effect directly imposed through promoters on the engineered gene expression is of par- ticular importance when developing yeast cell factories ...
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Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli
... than the other strains before it started to decrease. Inter- estingly, while the MVA levels in strain CL1605 were almost 20 times higher than that in strain CL1601 at the late fermentation phase, other metabolites such ...
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Tuning microbial hosts for membrane protein production
... netic point of view provide a similar cellular milieu as the protein's native host. Accordingly, this holds also true for lipid compositions of membranes and protein processing machineries. Many recent advances in ...
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