Influenza-virus polymerase
Investigation of Influenza Virus Polymerase Activity in Pig Cells
... enhances influenza virus polymerase activity in human or mouse cells, also increased activity in pig ...Eng195 polymerase, an influenza virus polymerase capable of ...
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The Replication Activity of Influenza Virus Polymerase Is Linked to the Capacity of the PA Subunit To Induce Proteolysis
... the influenza virus polymerase and NP pro- teins (pGPB1, pGPB2, pGPA, pGNP, and pGNPpA) have been described (26, ...␦ virus ribozyme and the T7 RNA terminator (1), into ...RNA ...
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Host and Strain Specific Regulation of Influenza Virus Polymerase Activity by Interacting Cellular Proteins
... HALo virus (i.e., virus modified by re- moval of the hemagglutinin [HA0] protein’s polybasic cleavage site) released from A549 cells ...H5N1 influenza virus polymerase activity in human ...
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Ubiquitination Upregulates Influenza Virus Polymerase Function
... the influenza A virus RNP complex are ubiquiti- ...with influenza virus, we detected unexpected higher-molecular-mass species of the polymerase subunits PB1 and PB2 ...viral ...
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Influenza virus polymerase basic protein 1 interacts with influenza virus polymerase basic protein 2 at multiple sites.
... its polymerase activity were studied by reconstitution of the active polymerase complex in ...for polymerase activ- ...for polymerase activity (12, ...the polymerase domain changed to ...
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Association of the Influenza A Virus RNA-Dependent RNA Polymerase with Cellular RNA Polymerase II
... that influenza virus transcription has a functional link with Pol II transcription ...enza virus RNA polymerase is indeed associated with Pol ...with influenza virus ...with ...
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Diversity of the RNA Polymerase in the H7N9 Influenza A Virus
... avian influenza viruses; the internal genes are derived from avian H9N2 viruses that circulated recently in China [8] ...The influenza virus polymerase complex, which is a heterotrimer formed ...
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Evidence for Avian and Human Host Cell Factors That Affect the Activity of Influenza Virus Polymerase
... the virus polymerase ...50-92 polymerase complex harboring the E627K mutation on PB2 was much more dramatic and reached 15-fold when the ratio of 293T to DF1 was highest ...Victoria polymerase ...
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Temperature-Sensitive Lesions in Two Influenza A Viruses Defective for Replicative Transcription Disrupt RNA Binding by the Nucleoprotein
... the influenza virus polymerase to switch from transcribing mRNAs to making replicative intermediate cRNAs remain relatively ill ...viral polymerase (29), and the direct participation of host ...
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Influenza Virus Nucleoprotein Interacts with Influenza Virus Polymerase Proteins
... the influenza virus transcription- replication assay in vivo, we incorporated the SA mutations in the context of whole NP and used a modified Ribo-CAT sys- tem for assaying transcription and replication as ...
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G Protein Pathway Suppressor 1 Promotes Influenza Virus Polymerase Activity by Activating the NF κB Signaling Pathway
... with virus in the same manner as in the previous experiments, and total RNA was extracted at 0, 3, 6, 9, and 12 ...influenza virus polymerase activity. (A) Virus polymerase activities in ...
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Three Amino Acid Substitutions in the L Protein of the Human Parainfluenza Virus Type 3 cp45 Live Attenuated Vaccine Candidate Contribute to Its Temperature-Sensitive and Attenuation Phenotypes
... L polymerase of PIV3 is a large polypeptide, 2,233 aa in length, and is thought to be a multifunctional protein that consists of multiple domains, including those re- quired for association with the P protein, RNA ...
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Identification of PB2 Mutations Responsible for the Efficient Replication of H5N1 Influenza Viruses in Human Lung Epithelial Cells
... The PB2 gene segment from 36285 is responsible for its effi- cient growth in human cells. To elucidate the molecular basis for the replicative difference between Wb/AH82 and 36285 in A549 cells, we generated a series of ...
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Adaptation of Avian Influenza A Virus Polymerase in Mammals To Overcome the Host Species Barrier
... of influenza A viruses lacking adaptive mutations that increase polymerase activity in mam- malian cells (1–53) are wild birds (54), mammals are frequently infected with influenza viruses of avian ...
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Characterization of Influenza Virus PB1 Protein Binding to Viral RNA: Two Separate Regions of the Protein Contribute to the Interaction Domain
... the polymerase is its binding to the 59 arm, since retention of the 39 probe was much more efficient when pre- sented to PB1 as a panhandle analog (a 59139 hybrid ...
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Heterocyclic pyrophosphate analogues as potential antiviral agents
... the virus alters the membrane permanently, this would account for an immediate difference in the uptake of small molecules which would proceed until the ...
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The Nucleoprotein of Newly Emerged H7N9 Influenza A Virus Harbors a Unique Motif Conferring Resistance to Antiviral Human MxA
... A polymerase reconstitution assay was performed as described in the legend of ...Relative polymerase activity was calculated as the ratio of luciferase activity in the presence of wild-type Mx1 compared to ...
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Individual expression of influenza virus PA protein induces degradation of coexpressed proteins.
... the polymerase subunits has proved to be a difficult task, particularly in the case of PA ...a virus-like CAT RNA ...a virus-infected cell? In the course of an influenza virus infec- ...
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Mutational Analysis of the Influenza Virus cRNA Promoter and Identification of Nucleotides Critical for Replication
... Theoretically it has been proposed that, unlike transcription, replication could occur without the involvement of the 5⬘ end of the RNA template (reviewed in reference 8). Our results (Fig. 2) with an in vivo primer ...
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Influenza Virus Polymerase Mutation Stabilizes a Foreign Gene Inserted into the Virus Genome by Enhancing the Transcription/Replication Efficiency of the Modified Segment
... insertion in their NS segment can propagate more efficiently in interferon (IFN)- deficient Vero cells than in IFN-competent cells such as MDCK cells (4, 13, 14). Therefore, we quantified the expression levels of IFN-  in ...
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