INS-1E
Role of MAPK in apolipoprotein CIII-induced apoptosis in INS-1E cells
... Protein extracts were prepared by lysis of INS-1E cells with a buffer composed of 150 mM NaCl, 50 mM Trizmabase, 1% Triton X100, 0.25% Na-deoxycholate, 1 mM Na 3 VO 4 , 2 mM EGTA and a protease inhibitory ...
6
AICAR and Metformin Exert AMPK-dependent Effects on INS-1E Pancreatic β-cell Apoptosis via Differential Downstream Mechanisms
... Sustained activation of JNK triggers apoptosis in response to multiple types of stress and has been proposed to mediate apoptosis in β-cells chronically exposed to palmitate [19, 20], which was successfully achieved in ...
9
High-dose clevudine impairs mitochondrial function and glucose-stimulated insulin secretion in INS-1E cells
... measured the released and cellular contents of insulin via an enzyme immunoassay. The cellular insulin con- tents were not significantly different between control INS-1E cells (816 ± 167 ng/well) and cells ...
9
Effects of zinc supplementation and zinc chelation on in vitro β cell function in INS 1E cells
... Using INS-1E cells, this study demonstrated that ma- nipulation of the zinc environment may affect β-cell survival and insulin production by interfering with intra- cellular zinc homeostasis under the ...
12
Lipotoxicity is glucose-dependent in INS-1E cells but not in human islets and MIN6 cells
... Free fatty acid preparation and cell/islet treatment Culture medium containing palmitate (Sigma, St. Louis, MO) was prepared as previously described [8,21]. Briefly, the fatty acid was dissolved in 50% ethanol to a ...
7
Proteins altered by elevated levels of palmitate or glucose implicated in impaired glucose-stimulated insulin secretion
... the present study, insulin content was severely reduced after extended hyperglycemia similar to what was previ- ously observed in INS-1E cells and primary islets [22,23]. In contrast, palmitate did not ...
11
UPR in palmitate-treated pancreatic beta-cells is not affected by altering oxidation of the fatty acid
... in time and leads to phosphorylation of eukaryotic initia- tion factor 2a (eIF2a), which attenuates protein synthesis and, at the same time, stimulates translation of ATF4. ATF4 is a transcription factor that regulates ...
8
Seven mutations in the human insulin gene linked to permanent neonatal/infancy onset diabetes mellitus
... Permanent neonatal diabetes mellitus (PNDM) is a rare disorder usually presenting within 6 months of birth. Although several genes have been linked to this disorder, in almost half the cases documented in Italy, the ...
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<p>IL6R inhibits viability and apoptosis of pancreatic beta-cells in type 2 diabetes mellitus via regulation by miR-22 of the JAK/STAT signaling pathway</p>
... Methods: The expressions of miR-22, IL6R and apolipoprotein (apoA1, apoB and apoE) were examined by reverse transcription-quantitative PCR (qRT-PCR). Pancreatic beta-cells were transiently transfected with a miR-22 mimic ...
13
Zinc transporter gene expression is regulated by pro-inflammatory cytokines: a potential role for zinc transporters in beta-cell apoptosis?
... h. INS-1E and INS-1E-ZnT8-EGFP cells were then treated with cytokines or control condition (no addition of cytokines) for 6, 12, and 24 ...
10
The ins and outs of Greek unemployment in the Great Depression
... dynamics. Finally, it should be noticed that in the crisis period, the indirect effects (for both inflows and outflows) appear to be very low and thus inadequate to explain unemployment variations. Panel B of Table 4 ...
39
Health Ins Booklet Plan 5
... For example, this plan covers certain preventive services without cost sharing and before you meet your deductible.. See a list of covered preventive services at www.healthcare.gov/cover[r] ...
7
Health Ins Booklet Plan 1
... In-network preventive care, in- network routine vision exams, in- network office/independent lab services for mental health/substance abuse and services subject to office visit/urgent ca[r] ...
8
Plug ins for GNU Radio Companion
... This paper gives an insight on how to develop plug-ins (signal processing blocks) for GNU Radio Companion. GRC is on the monitoring computer and does bulk of the signal processing before transmission and after ...
6
Child poverty: The shocking ins and outs
... the ins and outs of child poverty are ...the ins is seldom reflected. The ins is far more dangerous than the outs and is not easily traceable or ...the ins and bring solutions to them by ...
6
Lean Leadership - Organizational Buy - Ins
... buy ins from leadership which not only identifies strategies and processes for bringing quality which can then generate profits but identification of these resources is not only a difficult task because of the ...
7
Python How to Program, 1e pdf
... hardware platform high-level language input unit input/output I/O interpreter Java machine dependent machine independent machine language memory memory unit multiprocessor multiprogrammi[r] ...
1103
Expression pattern and regulation of genes differ between fibroblasts of adhesion and normal human peritoneum
... Relative abundance of specific mRNA species in the normal and adhesion fibroblasts. Genes differentially expressed between the normal and adhesion fibroblasts, as identified by gene filter experiments, were amplified by ...
14
The ins and outs of muscle stem cell aging
... For example, if the functional deficit of aged satellite cells (versus adult satellite cells) engrafted into an aged host was twice that observed in a young host, the results from parabi[r] ...
9