insect cells
Differential Incorporation of Cholesterol by Sindbis Virus Grown in Mammalian or Insect Cells
... other insect cell line, Sf21 from Spodoptera frugiperda; and BHK (mammalian) cells were ...in insect cells under normal culture conditions and in cells depleted of cholesterol by growth ...
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Generation of Replication-Competent Hepatitis B Virus Nucleocapsids in Insect Cells
... from insect cells, cis and trans cores contained Pol-linked HBV minus-strand DNA with 5 * ends mapping to the authentic elongation origin DR1 and also plus-strand DNA ...
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Responses of insect cells to baculovirus infection: protein synthesis shutdown and apoptosis.
... BmN cells (39). Transcription of mRNA in AcMNPV-infected BmN cells is apparently nor- mal ...BmN cells is induced by the putative helicase gene p143 of AcMNPV ...baculovirus-infected insect ...
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Espirito Santo Virus: a New Birnavirus That Replicates in Insect Cells
... from insect cells and other arthropod ...C6/36 cells have a capsid structure with a similar overall architecture but with underlying differences in the trimer contacts ...
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Bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression of the NS1 gene of bluetongue virus serotype 10.
... To demonstrate that BTV-10 NS1 protein was synthesized in recombinant baculovirus-infected cells, protein extracts were prepared from insect cells infected with the recombinant virus AcB[r] ...
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Optimization of recombinant human transferrin expression in insect cells baculovirus system
... To complement metabolic engineering works involving the humanization of recombinant glycoprotein, it is important that the recombinant protein can be generated in large quantities. The understanding of the insect ...
45
Purification and Characterization of Nipah Virus Nucleocapsid Protein Produced in Insect Cells
... protein was expressed in Sf9 insect cells by recombinant baculovirus. Western blot analysis of the recombinant fusion protein with anti-NiV antibodies produced a band of approximately 62 kDa. A time course ...
6
Yellow fever virus envelope protein expressed in insect cells is capable of syncytium formation in lepidopteran cells and could be used for immunodetection of YFV in human sera
... Tn5B cells (10 6 ) were co-transfected, using liposomes (CellFectin, Invitrogen), with the DNA (1 μg) from the plasmid pSynYFE and DNA (0,5 μg) from vSynVI - gal virus digested with Bsu36I, following the ...
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Mutational study of sapovirus expression in insect cells
... in insect cells only exam- ined rVP1 expression and yields of VLPs but not rVP1 mRNA transcription ...and insect cells, since a similar result was observed with porcine enteric calicivirus in ...
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Regulation of pp60c-src and its interaction with polyomavirus middle T antigen in insect cells.
... To determine whether i pp6Oc-src produced in insect cells was enzymatically active, ii MTAg could associate with any endogenous insect tyrosine kinases, and iii MTAg and pp60-csrc would [r] ...
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Structural Requirements of Astrovirus Virus-Like Particles Assembled in Insect Cells
... The role of divalent ions in the stabilization of VLPs is another intriguing issue. While the addition of EDTA to the purification buffers of sucrose gradients does not affect viral stabilization (6, 8), its inclusion in ...
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Retroviruses and insect cells
... disrupted cells and cell culture fluids were ...invertebrate cells or culture fluids assayed, capable of inducing focus or plaque formation under the conditions suitable for murine ...putative insect ...
300
Neutralization of poliovirus by cell receptors expressed in insect cells.
... When virus and S1OOPVR were incubated at 4 or 37°C and adsorbed to HeLa cells at 4°C, infectivity was reduced 10- and 1,000-fold, respectively Table 1, experiment 3, indicating that neut[r] ...
6
Characterisation of the adenovirus protease
... One of the substrates of the protease, the pre-terminal pTP protein was expressed in insect cells using a recombinant baculovirus.. Coinfections of cells with recombinant 23-kDa and pTP [r] ...
263
Identification and characterisation of the interaction between Wiskott-Aldrich syndrome protein (WASP) and c-Src kinases
... Sf9 cells by affinity purification using a phosphotyrosine ...and insect cells infected with the c-Fgr baculovirus. Cells were harvested, homogenised using a Dounce homogeniser in the absence ...
283
Wongabel Rhabdovirus Accessory Protein U3 Targets the SWI/SNF Chromatin Remodeling Complex
... B cells (30, 31), and the K8 protein of Kaposi’s sarcoma-associated herpesvirus interacts with the N-terminal half of SNF5 to facilitate transcrip- tional activation ...T cells and is in- corporated into ...
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Baculovirus gp64 gene expression: analysis of sequences modulating early transcription and transactivation by IE1.
... p64CAT constructs were expressed in uninfected cells of all three cell lines, indicating that in uninfected insect cells, transcription from the gp64 early promoter requires no other vir[r] ...
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Interactions of the Kaposi's Sarcoma-Associated Herpesvirus Nuclear Egress Complex: ORF69 Is a Potent Factor for Remodeling Cellular Membranes
... in insect cells using baculoviruses for protein expression forms a complex at the nuclear membrane and remodels these membranes to generate nuclear membrane-derived ...
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Expression of the Herpes Simplex Virus Type 2 Glycoprotein D in Baculovirus Expression System and Evaluation of Its Immunogenicity in Guinea Pigs
... In the present study, the Bac-to-Bac expression system was chosen because it is a valid and multi- purpose system for the production of recombinant proteins. The generation of recombinant baculoviruses by traditional ...
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Recombinant phospholipase A1 (Ves v 1) from yellow jacket venom for improved diagnosis of hymenoptera venom hypersensitivity
... Figure 4 IgE reactivity of patient sera. IgE reactivity of individual pa- tient sera from double-positive (A) or YJV-positive patients (B) to rVes v 1 and rVes v 5 produced in Sf9 insect cells, nApi m 1, ...
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