insect cells baculovirus system
Optimization of recombinant human transferrin expression in insect cells baculovirus system
... the baculovirus insect cell system has attracted wide attention as vectors for high level and faithful expression of a variety of heterologous ...
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Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
... (BSC-1 cells) were grown as a monolayer in DMEM (Himedia, India) supplemented with 10% Fetal Bovine Serum (FBS), 2 mM L-glutamine, and 100 U penicillin and 100 µg streptomycin per ml at ...infected cells ...
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Expression of the Herpes Simplex Virus Type 2 Glycoprotein D in Baculovirus Expression System and Evaluation of Its Immunogenicity in Guinea Pigs
... expression system was chosen because it is a valid and multi- purpose system for the production of recombinant ...a baculovirus shuttle vector (bacmid) propagated in ...this system, the ...
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Expression of Recombinant Small Hydrophobic Protein for Serospecific Detection of Avian Pneumovirus Subgroup C
... The baculovirus expression system has been used extensively to express large quantities of proteins that are antigenically similar to their native counterparts and can be used in stan- dardized assays to ...
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Rapamycin inhibition of baculovirus recombinant (BVr) ribosomal protein S6 kinase (S6K1) is mediated by an event other than phosphorylation
... mammalian cells, in complete dis- agreement with the conclusion drawn by some investi- gators ...in insect cells or inactivated due to viral infec- tion, undermining the existence of kinases ...
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Expression and characterization of hepatitis B virus surface antigen polypeptides in insect cells with a baculovirus expression system.
... The expression of two S polypeptides by recombinant viruses 373S and 106FS suggested that the polypeptides represented the unglycosylated p25 and glycosylated gp29 forms of the small HBs[r] ...
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Characterization of Neospora caninum Surface Protein NcSRS2 Based on Baculovirus Expression System and Its Application for Serodiagnosis of NeosporaInfection
... The baculovirus expression system has proved to be a useful tool for the production of recombinant ...in insect cells infected with each recombinant baculovirus, and a ...
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Production and purification of VP2 protein of porcine parvovirus expressed in an insect baculovirus cell system
... Currently, vaccines against PPV are produced by chemi- cally inactivating isolated virus particles grown in primary cell cultures of porcine origin. The method is both labor intensive and costly, with the additional ...
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Evaluation of an Enzyme Linked Immunosorbent Assay with Recombinant Rhoptry Associated Protein 1 Antigen against Babesia bovis for the Detection of Specific Antibodies in Cattle
... competent cells (Life ...cotransfect insect (Spodoptera frugiperda) cells (Sf9 cells) with baculovi- rus DNA by using liposome reagent (Cellfectin; Life ...Bac-to-Bac baculovirus ...
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Synthesis and immunogenicity of the rotavirus major capsid antigen using a baculovirus expression system.
... We report the successful production of the major capsid antigen VP6 of the simian rotavirus SAl1 in insect cells, using the baculovirus Autographa californica nuclear polyhedrosis virus [r] ...
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Characterization of Toxoplasma gondii SAG2 Expressed in Insect Cells by Recombinant Baculovirus and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay
... Sf9 cells infected with AcSAG2 were treated with or without PI-PLC, and then the cells and the medium were harvested separately and analyzed by Western blot analysis with anti- serum samples from mice ...
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High-Level Expression of Truncated Surface Antigen P50 of Babesia gibsoni in Insect Cells by Baculovirus and Evaluation of Its Immunogenicity and Antigenicity
... in insect cells. High Five (HF) cells infected with AcP50 or AcP50t (10 PFU/cell) were incubated with 1 ml of a protein-free Sf-900 II SFM medium (Gibco BRL, Rockville, ...infected cells were ...
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Identification and characterisation of the interaction between Wiskott-Aldrich syndrome protein (WASP) and c-Src kinases
... I initially was unable to co-immunoprecipitate endogenous WASP from cell extracts, with any of its potential binding partners. A variety of cell lines, subjected to different stimuli were used, together with various ...
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Nucleic Acid-Independent Retrovirus Assembly Can Be Driven by Dimerization
... overexpression system when sequences downstream of CA are deleted (11, 12, 16, 19, 27), and both HIV-1 and RSV assemble into tubes in vitro when sequences upstream of CA are deleted (5, 6, 10, 13–15, 18, ...18). ...
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The UL8 subunit of the herpes simplex virus helicase-primase complex is required for efficient primer utilization.
... We purified both the three-subunit helicase-primase complex and a UL5/UL52 subcomplex from recombinant baculovirus-infected insect cells and showed that the specific activities of the tw[r] ...
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Suppression of apoptosis in insect cells stably transfected with baculovirus p35: dominant interference by N-terminal sequences p35(1-76).
... By generating stably transfected SF21 cell lines, we have demonstrated for the first time that constitutive expression of baculovirus p35 protects insect lepidopteran cells from apoptosi[r] ...
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Biochemical and Biophysical Characterization of a Chimeric TRIM21-TRIM5α Protein
... Transiently transfected 293T cells expressing wild-type rhesus monkey TRIM5 ␣ were washed in PBS and lysed in NP-40 lysis buffer (0.5% Nonidet P40 [NP-40]–1⫻ protease inhibitor [complete EDTA-free, Roche ...
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Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
... As a general rule, proteolytic cleavages are essential for viral replication and many viruses encode their own protease. The exceptions do abound. Although the maturation of influenza viruses requires the cleavage of ...
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Replication, Integration, and Packaging of Plasmid DNA following Cotransfection with Baculovirus Viral DNA
... The insertion elements contained in the clones pM9, pM22, and pM25 as well as pM7 aligned with sequences from differ- ent organisms including humans, Caenorhabditis elegans, Dro- sophila melanogaster, and yeast. Some of ...
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Novel Baculovirus Expression Vectors That Provide Sialylation of Recombinant Glycoproteins in Lepidopteran Insect Cells
... Sf9 cells at 8 h postinfection and was sustained at high levels until at least 72 h postinfection ...immediate-early baculovirus expression vectors characterized in previous stud- ies (12, ...Sf9 ...
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