Interferon Gamma Release Assays
Point Counterpoint: Should Interferon Gamma Release Assays Become the Standard Method for Screening Patients for Mycobacterium tuberculosis Infections in the United States?
... CMI assays that were available required a 48-h antigen stimulation step, followed by tritiated thymidine ...these assays difficult for clinical laboratories to ...CMI assays have been developed that ...
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Comparison of the Cytomegalovirus (CMV) Enzyme Linked Immunosorbent Spot and CMV QuantiFERON Cell Mediated Immune Assays in CMV Seropositive and Seronegative Pregnant and Nonpregnant Women
... two interferon gamma release assays (IGRA), the CMV enzyme- linked immunosorbent spot (ELISPOT) and CMV QuantiFERON assays, detecting CMV-specific CMI were ...These assays were ...
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Serial interferon gamma release assays for latent tuberculosis in dialysis patients with end stage renal disease in a Korean population
... of interferon-gamma levels are more apparent in HD pa- tients than in PD patients ...IFN- gamma level of post-dialysis could be reduced compared with that of pre-dialysis ...
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Interferon Gamma Release Assays for Diagnosis of Pleural Tuberculosis: a Systematic Review and Meta Analysis
... of interferon gamma release assays for the diagnosis of tuberculous pleural effusion using whole-blood (top panel) or pleural fluid (bottom panel) ...QuantiFERON assays, ...
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Contribution of Interferon gamma release assays testing to the diagnosis of latent tuberculosis infection in HIV infected patients: A comparison of QuantiFERON TB Gold In Tube, T SPOT TB and tuberculin skin test
... The interferon-gamma release assays (IGRAs) measure T-cell release of interferon-γ (IFN-γ) following stimulation by antigens that are unique to ...these assays to detect ...
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Comparison of two interferon gamma release assays in the diagnosis of Mycobacterium tuberculosis infection and disease in The Gambia
... ELISPOT assays for IFN-γ were performed as previously described ...wells. Assays were counted with an automated ELISPOT reader (AID-GmbH, Strasberg, ...
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Role of interferon gamma release assays in the diagnosis of pulmonary tuberculosis in patients with advanced HIV infection
... feron-gamma release assays cannot distinguish immune responses among patients with latent infection, prior active disease, and current active disease when performed using peripheral blood specimens ...
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Comparing interferon gamma release assays with tuberculin skin test for identifying latent tuberculosis infection that progresses to active tuberculosis: systematic review and meta analysis
... treated with anti-TB prophylactic agents [7]. However evidence suggests that the currently available treatments for LTBI are effective in preventing a reactivation of TB (60–90%) [5], hence treatments would have had an ...
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Comparison of the QuantiFERON TB Gold Plus and QuantiFERON TB Gold In Tube Interferon Gamma Release Assays in Patients at Risk for Tuberculosis and in Health Care Workers
... Among the 161 subjects enrolled in our study, the QFT-GIT and QFT-Plus assays were qualitatively discordant in five (3.1%) individuals, and in all five cases, the QFT-GIT and QFT-Plus IFN- ␥ levels bordered the ...
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Poor agreement between interferon gamma release assays and the tuberculin skin test among HIV infected individuals in the country of Georgia
... As recommended by the manufacturer and the U.S. Centers for Disease Control and Prevention (CDC), [17] the QFT-GIT result was considered positive if the interferon-gamma response to TB antigens minus the ...
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Interferon Gamma Release Assays for Latent Tuberculosis: What Are the Sources of Variability?
... Interferon gamma release assays (IGRAs) are blood-based tests intended for diagnosis of latent tuberculosis infection (LTBI). IGRAs offer logistical advantages and are supposed to offer ...
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Systematic review and meta analysis on the utility of Interferon gamma release assays for the diagnosis of Mycobacterium tuberculosis finfection in children: a 2013 update
... of interferon-gamma release assays (IGRAs) for tuberculosis diagnosis in children yielded contrasting results, probably due to different inclusion/exclusion ...
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Detection of latent tuberculosis infection among migrant farmworkers along the US Mexico border
... There are two main diagnostic tools to detect LTBI: the tuberculin skin test (TST) and the interferon gamma release assays (IGRA) such as the Quantiferon TB gold- In-Tube (QFT-GIT). Both ...
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Persistent High Frequencies of Varicella-Zoster Virus ORF4 Protein-Specific CD4+ T Cells after Primary Infection
... infection. Gamma interferon (IFN- ␥ ) immunosorbent assays were used to screen peripheral blood mononu- clear cells obtained from healthy seropositive donors for responses to overlapping ORF4 ...
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Sensitivity Analysis and Potential Uses of a Novel Gamma Interferon Release Assay for Diagnosis of Tuberculosis
... ␥ assays, with their short incubation periods, measure mostly effector T-cell responses to antigens, as opposed to the TST, which measures both effector and memory T-cell responses ...
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The Association between Genetic Variation of Interferon Gamma +874 T/A and Plasma Interferon Gamma Level in Pulmonary Tuberculosis
... Abstract. These days, Mycobacterium tuberculosis (Mtb) bacil is estimated to have infected one third of the world's population. World Health Organization (WHO) data shows that in 2011 there were 8.7 million case of ...
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Apoptosis associated biomarkers in tuberculosis: promising for diagnosis and prognosis prediction
... Among the four groups (i.e., non-infection group, LTBI group, TB at diagnosis group, and TB under treat- ment group), inflammatory cytokines, including IL-6, TNF-alpha, and IFN-gamma decreased from the non- ...
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Comparison of Sensitivities of Two Commercial Gamma Interferon Release Assays for Pulmonary Tuberculosis
... commercial gamma interferon release assays ...(QFT-IT) assays in patients with culture-proven pulmonary ...both assays within 14 days of starting antituberculosis ...QFT-IT ...
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Tuberculosis infection risk, preventive therapy care cascade and incidence of tuberculosis disease in healthcare workers at Maputo Central Hospital
... Methods: This study was conducted among healthcare workers at Maputo Central Hospital, a public teaching quaternary care hospital in Mozambique. With a cross sectional study design, risk factors for tuberculosis were ...
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Screening travellers to high endemic countries for infection with Mycobacterium tuberculosis using interferon gamma release assay; a prospective study
... Interferon gamma release assay (IGRA), commercially available since 2005, is an in vitro T-cell based test which quantifies the immune response of T-cells to in vitro exposure to antigens of ...
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