Intergenic spacer
Use of Highly Variable Intergenic Spacer Sequences for Multispacer Typing of Rickettsia conorii Strains
... dksA-xerC spacer was composed of 63- to 102-bp repeat units ...the intergenic regions of bacterial genomes (59), but in ...dksA-xerC intergenic spacer were highly conserved and were present ...
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Rapid and accurate identification of Staphylococcus species by tRNA intergenic spacer length polymorphism analysis
... (tDNA) intergenic spacer length polymorphism (tDNA-ILP) was analyzed for its ability to identify to the species level type strains (n 5 18) and clinical isolates (n 5 163) of ...
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Simultaneous detection and genotyping of three genomic groups of Borrelia burgdorferi sensu lato in Dutch Ixodes ricinus ticks by characterization of the amplified intergenic spacer region between 5S and 23S rRNA genes
... Borrelia burgdorferi sensu lato, the causative agent of the zoonosis Lyme borreliosis (LB), is transmitted by ticks of the genus Ixodes (7). The onset of LB is often marked by the development of an erythema migrans rash ...
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Sequence Diversity of the Intergenic Spacer Region of the rRNA Gene of Malassezia globosa Colonizing the Skin of Patients with Atopic Dermatitis and Healthy Individuals
... on these results, M. globosa and M. restricta are thought to play a significant mycological role in AD. M. globosa is also part of the major microflora on the skin of healthy individuals. We used the intergenic ...
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High-Frequency Intragenomic Heterogeneity of the Ribosomal DNA Intergenic Spacer Region in Trichophyton violaceum
... The intergenic spacer (IGS) of the rRNA genes was analyzed from the dermatophyte Trichophyton violaceum isolated from cases of tinea capitis in Taiwan and ...
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Wood identification of JapaneseCyclobalanopsis species (Fagaceae) based on DNA polymorphism of the intergenic spacer betweentrnT andtrnL 5′ exon
... A part of the intergenic spacer region between trnT and trnL 5'exon was amplified and sequenced. gilva can be distinguished from the other Quercus species, and t[r] ...
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Evidence that intergenic spacer repeats of Drosophila melanogaster rRNA genes function as X-Y pairing sites in male meiosis, and a general model for achiasmatic pairing.
... Evidence That Intergenic Spacer Repeats of Drosophila melanogaster rRNA Genes Function as X-Y Pairing Sites in Male Meiosis, and a.. General Model for Achiasmatic Pairin[r] ...
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Sequence Analysis of the Ribosomal DNA Intergenic Spacer 1 Regions of Trichosporon Species
... We determined the sequence of the intergenic spacer (IGS) 1 region, which is located between the 26S and 5S rRNA genes, in 25 species of the genus Trichosporon. IGS 1 sequences varied in length from 195 to ...
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Identification of Acinetobacter isolates in the A calcoaceticus A baumannii complex by restriction analysis of the 16S 23S rRNA intergenic spacer sequences
... tween different isolates. This conclusion is rendered more in- teresting by the observation that the restriction patterns ob- tained with all of the endonucleases tested are often identical within the same DNA group ...
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Identification and detection of Trypanosoma cruzi by using a DNA amplification fingerprint obtained from the ribosomal intergenic spacer
... Identification and Detection of Trypanosoma cruzi by Using a DNA Amplification Fingerprint Obtained from the Ribosomal Intergenic Spacer.. We designed a PCR assay targeted on repeated el[r] ...
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Homogeneity of 16S 23S Ribosomal Intergenic Spacer Regions of Tropheryma whippelii in Swiss Patients with Whipple’s Disease
... the intergenic spacer region between the 16S and 23S rDNAs is usually much more variable and has repeatedly been used for epidemiologic ...the spacer region of ...the spacer sequence was also ...
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The calmodulin intergenic spacer as molecular target for characterization of Leishmania species
... The amplified products were submitted to 1% low melting point agarose gel in 1X TBE (89 mM Tris borate, 2 mM EDTA [pH 8.3]) electrophoresis and the product band was excised from agarose gel and purified using the ...
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Application of tRNA Intergenic Spacer PCR for Identification of Enterococcus Species
... tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of entero- coccal species of human and animal origin. This technique was carried out for 124 strains belonging ...
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PCR Analyses of tRNA Intergenic Spacer, 16S 23S Internal Transcribed Spacer, and Randomly Amplified Polymorphic DNA Reveal Inter and Intraspecific Relationships ofEnterobacter cloacae Strains
... tRNA intergenic spacer (tDNA-PCR) and of the 16S-23S internal transcribed spacer (ITS-PCR) and random amplified polymorphic DNA (RAPD) analysis were evaluated for their usefulness in characterization ...
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Borrelia carolinensis sp nov , a New (14th) Member of the Borrelia burgdorferi Sensu Lato Complex from the Southeastern Region of the United States
... rrf-rrl intergenic spacer region. The exact sizes of the rrf-rrl spacer regions (253 nucleotides [nt]) of the tested isolates were determined by direct sequencing of the purified ...rrf-rrl ...
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Variation in the Spacer Regions Separating tRNA Genes in Renibacterium salmoninarum Distinguishes Recent Clinical Isolates from the Same Location
... In this study tDNA PCR profiling was used to separate 22 isolates of R. salmoninarum from various sources in the United Kingdom into nine groups on the basis of the sizes of the amplicons which were generated using ...
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Species Identification and Subtyping ofUreaplasma parvum and Ureaplasma urealyticumUsing PCR Based Assays
... rRNA intergenic spacer regions, the urease gene subunits, and the 5 ⴕ ends of the multiple-banded antigen (MBA) genes, to identify and subtype these Ureaplasma ...
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Multispacer Typing To Study the Genotypic Distribution of Bartonella henselae Populations
... 16S-23S intergenic spacer (20), later permitted the identification of three, two, two, four, and six genotypes, respectively, that did not exactly match 16S rRNA gene ...
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Patterns of Variation in the Intergenic Spacers of Ribosomal DNA in Drosophila melanogaster Support a Model for Genetic Exchanges During X-Y Pairing
... in intergenic spacer (IGS) and internal transcribed spacer (ITS) regions of rDNA drawn from natural populations of Drosophila melanogaster has revealed contrasting patterns of homogenization although ...
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Serotype Identification of Group B Streptococci by PCR and Sequencing
... The new sequence data reported in this paper appear in the GenBank Nucleotide Sequence Database under accession numbers AF291411 to AF291419 for the 16S-23S rRNA intergenic spacer region[r] ...
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