Internal transcribed spacer regions
Typing of Histoplasma capsulatumIsolates Based on Nucleotide Sequence Variation in the Internal Transcribed Spacer Regions of rRNA Genes
... Histoplasma capsulatum causes histoplasmosis, a mycosis with an array of manifestations (19, 26). Some individuals may have no symptoms of infection. Some patients may experience mild flu-like symptoms or pneumonia, ...
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Detection and Identification of Mycobacteria by Amplification of the Internal Transcribed Spacer Regions with Genus and Species Specific PCR Primers
... ITS regions of 33 mycobacterial species using CLUSTAL-W ...ITS regions of ...ITS regions was analyzed with a BLAST search ...ITS regions in ...polymorphic regions of ITS sequences of ...
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Identification of Medically RelevantTrichosporon Species Based on Sequences of Internal Transcribed Spacer Regions and Construction of a Database forTrichosporon Identification
... the internal transcribed spacer (ITS) 1 and 2 regions in the rRNA gene were determined by directly sequencing PCR-amplified fragments for all of the species (17 species and five varieties) in ...
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Identification of Medically Important Yeast Species by Sequence Analysis of the Internal Transcribed Spacer Regions
... Many uncommon yeasts are emerging as human pathogens, and their identification may pose a challenge. A rapid and reliable identification method is urgently needed as the incidence of fun- gal infections increases (49). ...
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Identification of Aspergillus fumigatus and Related Species by Nested PCR Targeting Ribosomal DNA Internal Transcribed Spacer Regions
... Aspergillus fumigatus is the most common species that causes invasive aspergillosis. In order to identify A. fumigatus, partial ribosomal DNA (rDNA) from two to six strains of five different Aspergillus species was ...
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Update on Pneumocystis carinii f sp hominis Typing Based on Nucleotide Sequence Variations in Internal Transcribed Spacer Regions of rRNA Genes
... ITS regions were first probed with type- specific probes 1A, 1B, 2a, 2b, and 2c to determine whether the specimen contained a single or multiple types of ...
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Internal Transcribed Spacer Regions of rRNA Genes of Pneumocystis carinii from Monkeys
... Since both the ITS1 and ITS2 sequences of specimen C375 are quite different from those of the other 13 speci- mens, we propose to classify P. carinii f. sp. macacae isolates into two major groups. Group I consists of the ...
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Rapid Identification and Differentiation of Trichophyton Species, Based on Sequence Polymorphisms of the Ribosomal Internal Transcribed Spacer Regions, by Rolling Circle Amplification
... DNA sequencing analyses have demonstrated relatively limited polymorphisms within the fungal internal transcribed spacer (ITS) regions among Trichophyton spp. We sequenced the ITS region ...
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Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2
... intergenic spacer region, and the internal transcribed spacer (ITS) ...ITS regions are located between the 18S and 28S rRNA genes and offer distinct advantages over other molecular ...
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High Throughput Detection of Pathogenic Yeasts of the Genus Trichosporon
... The need for a rapid and accurate method for the detection of fungal pathogens has become imperative as the incidence of fungal infections has increased dramatically. Herein, we tested the Luminex 100, a novel flow ...
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Genotypic Study of Pneumocystis jirovecii in Human Immunodeficiency Virus Positive Patients in Thailand
... Pneumocystis jirovecii is one of the common opportunistic infections in human immunodeficiency virus (HIV)-infected patients in Thailand. Information regarding genotypic and epidemiological of this organism in Thai ...
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Sequence Analysis of the Ribosomal DNA Intergenic Spacer 1 Regions of Trichosporon Species
... intergenic spacer (IGS) 1 region, which is located between the 26S and 5S rRNA genes, in 25 species of the genus ...the internal transcribed spacer ...IGS regions provides a powerful ...
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Comparison of internal transcribed spacers and intergenic spacer regions of five common Iranian sheep bursate nematodes.
... (Internal transcribed spacer 1, 2 and Inter- genic spacer) are suitable for accurate identification of sheep bursate ...IGS regions of Iranian common bursate nematodes in order to ...
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Detection of Kudoa septempunctata in the Japanese Flounder by LoopMediated Isothermal Amplification (LAMP) Targeting the Internal Transcribed Spacer (ITS) Regions
... Four specific LAMP primers composed of two outer primers (Ks-F3 and Ks-B3) and two inner primers (Ks-FIP and Ks-BIP) were designed based on the conserved sequence (among different strains, Figure 2) of the ITS1 region of ...
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Multiplex PCR Using Internal Transcribed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains
... Multiplex PCR amplification followed by either agarose gel electrophoresis (PCR-AGE) or microchip electrophoresis (PCR-ME) was used to test a total of 120 fungal strains. The internal transcribed ...
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Genetic Diversity of Pneumocystis carinii f sp hominis Based on Variations in Nucleotide Sequences of Internal Transcribed Spacers of rRNA Genes
... ITS2 internal transcribed spacer (ITS) regions of the rRNA genes were used to type Pneumocystis carinii ...These regions were amplified by PCR, cloned, and ...ITS2 regions in ...
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Phylogenetic Analysis of The Internal Transcribed Spacer (ITS) Region in Laccase Producing Pseudomonas aeruginosa ADN04 by Predicted rRNA Secondary Structure
... The internal transcribed spacer (ITS) regions, which are scattered among the mature rRNA genes are because they enable their own excision from the RNA transcript, have been sequenced widely ...
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Patterns of Variation in the Intergenic Spacers of Ribosomal DNA in Drosophila melanogaster Support a Model for Genetic Exchanges During X-Y Pairing
... intergenic spacer (IGS) and internal transcribed spacer (ITS) regions of rDNA drawn from natural populations of Drosophila melanogaster has revealed contrasting patterns of ...
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Genetic comparison of different human population groups using internal transcribed spacer sequence
... Genetic identity testing for humans has been used to examine the variations in the polymorphic regions of the human DNA. These methods use unique genetic markers such as the RFLP (Restriction fragment length ...
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PCR Analyses of tRNA Intergenic Spacer, 16S 23S Internal Transcribed Spacer, and Randomly Amplified Polymorphic DNA Reveal Inter and Intraspecific Relationships ofEnterobacter cloacae Strains
... co- transcribed in a single operon in ...by internal spacer regions (ITS), which exhibit a large degree of sequence and length variation at the levels of genus and ...the spacer may ...
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