Intracellular protein
Survey of Intracellular Protein Extraction Methods from Pichia pastoris
... the intracellular protein release yield also increased accordingly (Table ...and protein release yield were not significantly improved when homogenized time were more than ...and protein ...
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Silica Nanoparticles for Intracellular Protein Delivery: a Novel Synthesis Approach Using Green Fluorescent Protein
... fluorescent protein (GFP)-doped silica nanoparticles with a narrow size distribution is ...model protein due to its autofluorescence. Protein-doped nanoparticles have a high application potential in ...
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AN INTRACELLULAR PROTEIN INTERMEDIATE FOR HEMOGLOBIN FORMATION
... It is interesting to note that a highly labeled young red cells have been described: the transfer protein closely related to globin present in young of ferritin from macrophages to young[r] ...
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Intracellular protein determination using droplet-based immunoassays
... fusion protein expression varied from cell to cell, and that the number of cells being lysed at the one time was variable - typically 1-3 cells were trapped by the electrodes at the same time ...
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Scale-down of a bioprocess sequence for the recovery and purification of an intracellular protein
... total protein and ADH precipitated at each cut ...total protein) relative to the homogenate for each ...soluble protein in the feed entrained with recovered solids for C4 ...
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Selective and Potent Proteomimetic Inhibitors of Intracellular Protein-Protein Interactions
... Abstract: Inhibition of protein–protein interactions (PPIs) represents a major challenge in chemical biology and drug discovery. a-Helix mediated PPIs may be amenable to modulation using generic chemotypes, ...
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Alveolar cells: incorporation of carbohydrate into protein and evidence for intracellular protein transport
... microsomal protein and the protein of particles sedimenting at 15,000 ...into protein at the microsomal level with subsequent transfer of this newly formed material to particles sedimenting at 15,000 ...
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Selective and potent proteomimetic inhibitors of intracellular protein-protein interactions
... Abstract: Inhibition of protein–protein interactions (PPIs) represents a major challenge in chemical biology and drug discovery. a-Helix mediated PPIs may be amenable to modulation using generic chemotypes, ...
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Atrogin 1 deficiency promotes cardiomyopathy and premature death via impaired autophagy
... body protein 2B (CHMP2B), which is part of an endosomal sorting complex (ESCRT) required for autophagy, as a target of atrogin-1–mediated ...impairment, intracellular protein aggregate accumulation, ...
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Stable isotope dynamic labeling of secretomes (SIDLS) identifies authentic secretory proteins released by cancer and stromal cells
... labeled intracellular proteins will enter and equilibrate with the unlabeled pre- existing ...unlabeled protein, and thus the RIA is ...of protein molecules that are labeled will be ...large ...
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Expression and intracellular localization of duck enteritis virus pUL38 protein
... finding indicated that pUL38 is expressed throughout the viral replication cycle. The expression of Herpesviri- dae genes is temporally controlled and coordinated in a cascade fashion [19]. Immediate-early (a) ...
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<p>Virus–Receptor Interactions: Structural Insights For Oncolytic Virus Development</p>
... gD protein arises from a crystal structure of gD ectodomain truncated at residues 285 (gD285) bound to the ectodomain of HVEM (Figure ...gD protein is involved in HVEM binding suggests the possibility of ...
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Assembly and Intracellular Localization of the Bluetongue Virus Core Protein VP3
... on protein-protein in- teraction and BTV particle assembly (11, 23, 24, 33, ...Coomassie-stained protein bands of purified CLPs was performed to estimate the proportion of VP7 in each preparation ...
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Intracellular Localization of the Pseudorabies Virus Large Tegument Protein pUL36
... on intracellular localization of pUL36 published to date rely on several antibodies directed against different fragments of this huge protein revealing different intracellular ...the protein. ...
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Evidence for a separate signal sequence for the carboxy-terminal envelope glycoprotein E1 of Semliki forest virus.
... For examination of the reactivity of intracellular proteins with antiserum prepared against 29S envelope protein octamers 26 and antisera prepared against isolated El, E2, and E3 protein[r] ...
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Intracellular Trafficking Governs the Processing of the Amyloid Precursor Protein and the Secretion of Beta-Amyloid
... on intracellular APP trafficking, we transiently transfected SN56 cells with βAPPsw-paGFP along with plasmids expressing compartment markers for the Golgi apparatus and ...lysosomes. Protein from SN56 cells ...
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Use of a Recombinant Burkholderia Intracellular Motility A Protein for Immunodiagnosis of Glanders
... Burkholderia intracellular motility A (BimA) protein, also called the hemagglutinin domain-con- taining protein, is located on the pole of bacterium ...autosecreted protein, and the amino ...
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Intracellular Trafficking Network of Protein Nanocapsules: Endocytosis, Exocytosis and Autophagy
... delivering protein receptors back to the cell membrane ...the intracellular trafficking and, accordingly, the design of nanocarriers is limited to the EEs-LEs-lysosome ...
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Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts
... and protein expression upon the treatment of HB, LED and HB-LED ...and protein expression was examined using flow cytometry intracellular staining ...cytometry intracellular staining, in which ...
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The Envelope G3L Protein Is Essential for Entry of Vaccinia Virus into Host Cells
... G3L protein was expressed, the vaccinia virus life cycle proceeded normally, resulting in plaque formation in BSC40 ...G3L protein expression was repressed, no plaques were formed, showing that G3L ...
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