Intron Retention
Intron Retention May Regulate Expression of Epstein-Barr Virus Nuclear Antigen 3 Family Genes
... Thus, intron retention can regulate gene expression without changing transcriptional ac- ...the intron would effectively disrupt the translation ...an intron-containing mRNA was associated ...
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DMD transcripts in CRL-2061 rhabdomyosarcoma cells show high levels of intron retention by intron-specific PCR amplification
... with retention of introns 40 and ...by intron retention that generated a premature stop codon. Intron specific PCR amplification of nine short introns, showed retention of introns 40, ...
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The Y Chromosome Modulates Splicing and Sex-Biased Intron Retention Rates in Drosophila
... ABSTRACT The Drosophila Y chromosome is a 40-Mb segment of mostly repetitive DNA; it harbors a handful of protein-coding genes and a disproportionate amount of satellite repeats, transposable elements, and multicopy DNA ...
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Position-specific intron retention is mediated by the histone methyltransferase SDG725
... different intron length ...(blue.) intron retention (IR) events between ...(blue.) intron retention (IR) events in genes with no obvious changes ( ≤ 2-fold) in total levels of H3K36me2 ...
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IRFinder: assessing the impact of intron retention on mammalian gene expression
... To validate the results of our algorithm we used RT-qPCR to measure intron retention in two cases where IR detec- tion can be problematic. The first validation was per- formed on low IR levels, the second ...
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Intron retention in Cathelicidin-4 in river buffalo
... of intron 3 (469 bp) in addition to previously reported unspliced complete intron 1 (103) and intron 2 (137 ...of intron retention was conducted by comparing the amplified unspliced ...
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Widespread intron retention diversifies most cancer transcriptomes
... alter intron rec- ...increased intron retention in clear cell renal cell carcinoma [19], our results suggest that crosstalk between epigenetics and RNA splicing may link a diverse spectrum of ...
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Gene activity in primary T cells infected with HIV89 6: intron retention and induction of genomic repeats
... of intron retention as a consequence of HIV infection, (2) the finding of acti- vation of ERV-9/LTR12C after HIV infection, (3) gen- eration of a quantitative account of the structures and abundances of ...
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Herpes Simplex Virus Type 1 ICP27 Regulates Expression of a Variant, Secreted Form of Glycoprotein C by an Intron Retention Mechanism
... We previously showed that herpes simplex virus type 1 (HSV-1) immediate-early (IE) protein ICP27 can posttranscriptionally stimulate mRNA accumulation from a transfected viral late gene encoding glycoprotein C (gC) (K. ...
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Intron retention enhances gene regulatory complexity in vertebrates
... containing intron should be required. Surprisingly, in intron-retaining genes the number of retained introns is proportional to the total number of introns in a gene (Additional file 2: Figure ...one ...
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Minor spliceosome inactivation causes microcephaly, owing to cell cycle defects and death of self amplifying radial glial cells
... elevated retention in the mutant, we employed Student ’ s t-tests to compare the MSI of each minor intron between the control and mutant ...minor intron retention in the mutant (Table ...Minor ...
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The global landscape of intron retentions in lung adenocarcinoma
... somatic intron re- tentions, the most intuitive explanation is that somatic mutations occur at splicing sites and alternate the spli- cing signal, therefore those splicing sites could not be properly ...that ...
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Splice-site mutation causing partial retention of intron in the FLCN gene in Birt-Hogg-Dubé syndrome: a case report
... partial intron retention (130 bp) and the following 104 bp of exon 10, which ended at a stop codon, pre- dicted the translation of an additional 77 amino acids between intron 9 (43 amino acids) and ...
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Unproductive alternative splicing and nonsense mRNAs: A widespread phenomenon among plant circadian clock genes
... Results: We conducted a systematic survey of alternative splicing of plant circadian clock genes using RNA-seq and found that many Arabidopsis thaliana circadian clock-associated genes are alternatively spliced. Results ...
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Transcriptome-Wide Analysis Of Hypoxic Cancer Cells Identify Alternative Splicing As A Mechanism To Inhibit Translation
... retained intron in the master regulator of translation initiation, ...retained intron in EIF2B5 leads to a truncated isoform that opposes full-length eIF2Bε to inhibit ...influence intron ...
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A novel RNA variant of human concentrative nucleoside transporter 1 (hCNT1) that is a potential cancer biomarker
... the intron retention variant hCNT1-1R ...hCNT1 intron retention variant, hCNT1-IR also shares similarities with that of rCNT1 except like with hCNT1, its significant overexpression expression ...
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Expression and Characterization of Six Mutations in the Protoporphyrinogen oxidase gene among Finnish Variegate Porphyria Patients
... that intron retention is permitted only when the intron is small enough to satisfy the exon length ...4, intron 4, and exon 5 was 756 bp, which possibly violates the length limitation and thus ...
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U12 intron positions are more strongly conserved between animals and plants than U2 intron positions
... of intron-poor organisms display a substantial bias of intron distribution over the coding sequence length, with introns strongly over-represented in the 5'-portion of the genes, an obser- vation that ...
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Intron Definition Is Required for Excision of the Minute Virus of Mice Small Intron and Definition of the Upstream Exon
... Both small-intron acceptors have what appear to be poor PPTs. Both are very short and have several purine interrup- tions (Fig. 3a). A PPT of at least five consecutive uridines or nine consecutive pyrimidines is ...
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Polymorphism in the third intron of the interferonγ gene is associated with susceptibility to multiple sclerosis.
... Studies have shown that intron III of IFN-γ has three SNP sites (+2118 site A/G, +2707 site G/A, and +3586 site G/ACT). In our previous study, we found that +2707 A/G was not associated with susceptibility to MS. ...
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