invasion assay
Galangin, a novel dietary flavonoid, attenuates metastatic feature via PKC/ERK signaling pathway in TPA-treated liver cancer HepG2 cells
... The invasion assay was performed by using Hanging Cell Culture-inserts (BD Biosciences; pore size, 8 μm) in a 6-well plate. The ability of HepG2 cells to pass through filters coated with Matrigel was ...
11
RETRACTED ARTICLE: The microRNA-325 inhibits hepatocellular carcinoma progression by targeting high mobility group box 1
... blot assay was used to examine the transfection efficiency ...CCK8 assay and transwell invasion assay showed that down-regulation of HMGB1 partially attenuated the in- vasion and proliferation ...
11
Original Article Effect of lysozyme on invasion and migration of human lung carcinoma A549 cells
... on invasion and migration of the human lung carci- noma A549 ...on invasion ability of the A549 cells was detected by Transwell invasion assay; effect of LYZ expression silence on migration ...
8
Comparative Analysis of Pathogenicity Assays and PCR for Listeria monocytogenes Isolated from Animal, Raw Food and Environmental Sources in Korea
... More recently, test methods based on detection of spe- cific virulence genes may offer alternative approaches for identifying of L. monocytogenes virulence [15]. We se- lected two genes (lmo2672 and lmo1134) encoding ...
9
Long non-coding RNA MALAT1 drives gastric cancer progression by regulating HMGB2 modulating the miR-1297
... cell invasion assay also revealed that knockdown of MALAT1 efficiency inhibited the cell invasion abilities in MKN45 or MKN28 cells, and this inhibition could be restored by co-transfecting with ...
9
The inhibition role of miR-22 in hepatocellular carcinoma cell migration and invasion via targeting CD147
... RT‑PCR. Invasion assay, MTT proliferation assay and wound‑healing assay were performed to test the invasion and proliferation of HCC cell after overexpression of ...and invasion ...
9
ISL1 is upregulated in breast cancer and promotes cell proliferation, invasion, and angiogenesis
... and invasion by wound healing and transwell invasion ...healing assay demonstrated that the siISL1 decreased inclosure of cells compared with the SCR group ...transwell invasion assay ...
9
<p>FOXK1, Regulated by miR-365-3p, Promotes Cell Growth and EMT Indicates Unfavorable Prognosis in Breast Cancer</p>
... Transwell invasion assay demonstrated that up-regulation of miR- 365-3p reversed the effects of FOXK1 on proliferation and invasion in MDA-MB-231 cells, and inhibition of miR-365-3p reversed the ...
12
Down-regulation of ARNT promotes cancer metastasis by activating the fibronectin/integrin β1/FAK axis
... 107 inhibited ARNT expression, and the inhibition was reversed by a miR-107 inhibitor (Figure 8B). Compared to ARNT-Myc, the expression level of ARNT-Myc with a 3′UTR (ARNT-Myc 3′UTR) was significantly decreased (Figure ...
17
Original Article Predictive value of miR-27b as a potential biomarker for head and neck squamous cell carcinoma
... MTT assay, cell counting assay and invasion assay were used to assess the effects of miR-27b upregulation or downregulation on the proliferation and invasion of HNSC ...and ...
6
Original Article Long chain non coding TUG1 regulating lung cancer invasion and proliferation by targeting Mir-300 in A549 cell line
... the invasion and proliferation of lung cancer ...Transwell invasion assay was used to detect the invasion ability of lung cancer cells after silencing TUG1, and the platelet clone formation ...
10
Knockdown of a DIS3L2 promoter upstream long noncoding RNA (AC105461.1) enhances colorectal cancer stem cell properties in vitro by down-regulating DIS3L2
... proliferation assay, matrigel invasion assay, and spheroid formation as well as flow cytometry analysis were performed respectively to investigate the effect of ...
10
Ghrelin Regulates Energy Homeostasis Through Discrete Central and Peripheral Signaling Mechanisms
... affinity assay and five out of those six compounds performed well in Matrigel invasion ...affinity assay were 4-Me (3d) and 4-Cl (3l) with an effective concentration of 10 ...Matrigel invasion ...
161
<p>MicroRNA-432 Suppresses Invasion and Migration via E2F3 in Nasopharyngeal Carcinoma</p>
... healing assay and Transwell invasion assay showed that E2F3 knockdown suppressed NPC cell invasion and migra- tion in vitro (Figure 2E and ...
10
RNAi silencing of c-Myc inhibits cell migration, invasion, and proliferation in HepG2 human hepatocellular carcinoma cell line: c-Myc silencing in hepatocellular carcinoma cell
... vitro invasion assay was designed to test whether transfection of siRNA c-Myc altered the in- vasive potential of tumor cells in Matrigel-coated transwell ...
6
Original Article Osteopontin facilitates invasion in human trophoblastic cells via promoting matrix metalloproteinase-9 in vitro
... blast invasion, maternal-embryo interaction and immune ...phoblast invasion, the process of trophoblast invasion bears several striking similarities to tumor cell metastasis, except the trophoblast ...
10
Toxoplasma gondii Targets a Protein Phosphatase 2C to the Nuclei of Infected Host Cells
... In order to enter and survive within its host cells, Toxoplasma actively subverts host defenses and co-opts host cell processes. While the host response is understood to some extent, little is known about the specific ...
11
PIM1 regulates glycolysis and promotes tumor progression in hepatocellular carcinoma
... The media of cell culture (both normoxic and hypoxic samples) were removed and rinsed with ice- cold phosphate-buffered saline (PBS). Cellular extracts were collected on ice using RIPA buffer (0.5% sodium deoxycholate, ...
13
<p>Arsenic sulfide induces miR-4665-3p to inhibit gastric cancer cell invasion and migration</p>
... Gastric cancer (GC) is the second leading cause of cancer death and the fourth most common malignancy all over the world. 1 Approximately 50% of all gastric cancers occurring in Asia, especially in China, Japan and ...
13
The lncRNA DLX6-AS1 promoted cell proliferation, invasion, migration and epithelial-to-mesenchymal transition in bladder cancer via modulating Wnt/β-catenin signaling pathway
... Collection of clinical tissues from bladder cancer patients The experimental protocols for this study were under the approval of Ethics Committee of Shenzhen People’s Hospital, and the experiments were undertaken with ...
11