IS6110-RFLP
Comparative Evaluation of Ligation Mediated PCR and Spoligotyping as Screening Methods for Genotyping ofMycobacterium tuberculosis Strains
... between IS6110 RFLP patterns and the cor- responding LMPCR results was found to be higher than that reported by Prod’hom et ...other IS6110-based PCR methods with nonspecific priming problems (26), ...
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Improvement of Differentiation and Interpretability of Spoligotyping for Mycobacterium tuberculosis Complex Isolates by Introduction of New Spacer Oligonucleotides
... different IS6110 RFLP patterns; one strain con- tained a single IS6110 element, which gave a different pattern with novel probe ...two IS6110 copies with different spoligotype ...the ...
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Genetic Heterogeneity in Mycobacterium tuberculosisIsolates Reflected in IS6110 Restriction Fragment Length Polymorphism Patterns as Low Intensity Bands
... 1,277 IS6110 RFLP patterns no mul- tiply banded background patterns, indicative of tuberculosis infections with more than one strain, were ...for RFLP typing originated from a second ...of ...
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Comparison of Variable Number Tandem Repeat and IS6110 Restriction Fragment Length Polymorphism Analyses for Discrimination of High and Low Copy Number IS6110 Mycobacterium tuberculosis Isolates
... different IS6110- RFLP profiles but still share the same VNTR profile (10, 18, 20, ...an IS6110-RFLP pattern (1, 5, 23, 24, 34, ...that IS6110-RFLP fingerprints in docu- mented ...
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Genetic Diversity of Mycobacterium tuberculosis Isolates from a Tertiary Care Tuberculosis Hospital in South Korea
... Interestingly, this grouping based on IS6110-RFLP analysis was congruent with the grouping obtained using a minimum spanning tree based on MIRU-VNTR data (Fig. 2). This con- gruence demonstrates the ...
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Genetic Diversity of Mycobacterium tuberculosis in Sicily Based on Spoligotyping and Variable Number of Tandem DNA Repeats and Comparison with a Spoligotyping Database for Population Based Analysis
... sequence IS6110 is considered a “gold standard” among DNA fingerprinting methods (23, 30), a number of limitations and drawbacks of IS6110 fingerprinting have been demonstrated, both at the theoretical ...
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Fast Ligation Mediated PCR, a Fast and Reliable Method for IS6110 Based Typing of Mycobacterium tuberculosis Complex
... berculosis strain from India (number 36) exhibited a single band of identical size to those of the M. bovis and M. bovis BCG strains in the study (Fig. 3B), while these strains each showed a band at another position in ...
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Automated High Throughput Genotyping for Study of Global Epidemiology of Mycobacterium tuberculosis Based on Mycobacterial Interspersed Repetitive Units
... and IS6110 RFLP analysis, observed for only a minority of isolates, may be due to several ...few IS6110 bands with the other strains of the complex (for instance, Mycobacterium microti isolates 25 ...
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Multiple Mycobacterium tuberculosis Strains in Early Cultures from Patients in a High Incidence Community Setting
... with IS6110 have illustrated the presence of more than one ...identical, IS6110 RFLP pattern were cultured from a patient’s sputum samples (3), while another patient produced a culture containing two ...
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Evaluation of a High Throughput Repetitive Sequence Based PCR System for DNA Fingerprinting of Mycobacterium tuberculosis and Mycobacterium avium Complex Strains
... with RFLP clusters A, B, C, and NT ...using IS6110-RFLP as the high- resolution adjunct, the only two differences being that Cluster A contained four strains by RFLP and three strains by rep- ...
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Genetic Diversity of Mycobacterium africanum Clinical Isolates Based on IS6110 Restriction Fragment Length Polymorphism Analysis, Spoligotyping, and Variable Number of Tandem DNA Repeats
... on IS6110-restriction fragment length polymorphism (IS6110- RFLP) analysis, IS1081-RFLP analysis, direct repeat-based spacer oligonucleotide typing (spoligotyping), variable number of tandem ...
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Mycobacterial Interspersed Repetitive Unit Typing of Mycobacterium tuberculosis Compared to IS6110 Based Restriction Fragment Length Polymorphism Analysis for Investigation of Apparently Clustered Cases of Tuberculosis
... the IS6110 RFLP method has been carefully stan- dardized ...centers. IS6110 RFLP has been applied in many settings such as regional epidemiological stud- ies (3, 19), point source outbreaks ...
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Evaluation of spoligotyping in a study of the transmission of Mycobacterium tuberculosis
... by IS6110-based restriction fragment length polymorphism (RFLP) ...same IS6110-RFLP cluster were also in the same spoligotyping ...than RFLP analysis for strains with one or two copies ...
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Stability of IS6110 Restriction Fragment Length Polymorphism Patterns of Mycobacterium tuberculosisStrains in Actual Chains of Transmission
... patient per cluster (index patient) from the total number of patients (number of changed isolates/total number of iso- lates ⫺ total number of clusters) or 11% if only the corre- sponding cluster was regarded. The ...
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Genotypic and Phenotypic Heterogeneity among Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients
... the IS6110- RFLP patterns in order to assess the adequacy of IS6110 fin- gerprinting to track the recent transmission of TB (6, 23, ...different IS6110-RFLP patterns and/or ...
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Chromosomal DNA fingerprinting analysis using the insertion sequence IS6110 and the repetitive element DR as strain specific markers for epidemiological study of tuberculosis in French Polynesia
... sequence IS6110 and the repetitive element DR as ...different IS6110 RFLP types were ...identical RFLP types between patients of the same family or between patients living in the same area was ...
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Comparison of Methods Based on Different Molecular Epidemiological Markers for Typing of Mycobacterium tuberculosis Complex Strains: Interlaboratory Study of Discriminatory Power and Reproducibility
... five RFLP typing methods were highly ...(DRE-PCR), IS6110 inverse PCR, IS6110 ampliprinting, and arbitrarily primed PCR (APPCR) typing were found to be poorly ...by IS6110 RFLP typing, ...
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Three Year Population Based Evaluation of Standardized Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat Typing of Mycobacterium tuberculosis
... standard IS6110-RFLP or MIRU- VNTR typing based on 12 loci plus spoligotyping, (ii) the assessment of standardized MIRU-VNTR typing for phylogeo- graphical analysis, and (iii) a test of the robustness and ...
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Sensitivities and Specificities of Spoligotyping and Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat Typing Methods for Studying Molecular Epidemiology of Tuberculosis
... and IS6110-based RFLP have been seen as roughly equiv- alent (6, 7, 8, 16, ...of IS6110 RFLP results was considerably greater than that of MIRU-VNTR results ...that IS6110 RFLP ...
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Predominance of Mycobacterium tuberculosis EAI and Beijing Lineages in Yangon, Myanmar
... Table 1 shows the ST distributions of M. tuberculosis isolates belonging to the two most commonly circulating lineages (Bei- jing and EAI) in Yangon. ST1 (Beijing lineage) was the most prevalent ST among the isolates in ...
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