Islet Isolation
The long noncoding RNA MALAT1 predicts human islet isolation quality
... although islet autotransplants (in chronic pancreatitis) are reimbursable, islet allotransplanta- tion in individuals with T1D is performed only through clinical ...of islet isolation from a ...
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A Practical Guide to Rodent Islet Isolation and Assessment
... rat islet isolation, including choices of collagenase, the collagenase digestion process, purification of islets using a density gradient, and islet culture ...assessing islet viability and ...
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Optimization of pancreatic islet isolation from rat and evaluation of islet protective potential of a saponin isolated from fruits of Momordica dioica
... the isolation of 200–400 ...time. Islet beta-cell replacement and regeneration are the keys approached for the treatment of diabetic ...the islet isolation was ...
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Introducing a New Experimental Islet Transplantation Model using Biomimetic Hydrogel and a Simple High Yield Islet Isolation Technique
... In the conventional islet isolation techniques, large amounts of enzyme and multiple toxic density gradients of histopaque are needed. Furthermore, in situ pancreas perfusion through common bile duct or ...
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Testing of a New Collagenase Blend for Pancreatic Islet Isolation Produced by Clostridium histolyticum
... pancreatic islet isolation and the parallel results were used as a feed-back for modification of the culture and purification method in order to achieve stable results in terms of the islet ...
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Robot assisted pancreatoduodenectomy with preservation of the vascular supply for autologous islet cell isolation and transplantation: a case report
... In addition to providing a platform to perform a mini- mally invasive pancreatectomy in the technically demanding setting of CP, robot assistance offers the possibility to preserve the vascular supply of the pan- creatic ...
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Clathrin-mediated Endocytosis of Alpha-1 Antitrypsin is Essential for its Protective Function in Islet Cell Survival
... with islet isolation and islet ...in islet transplantation and ...improved islet graft survival after syngeneic islet transplantation in mice receiving islets isolated from AAT ...
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Islet cell transplantation for the treatment of type 1 diabetes: recent advances and future challenges
... successful islet isolation and ...the islet mass transplanted does not unequivocally correlate with islet graft ...for islet transplantation and hence allocation rules (pancreas for ...
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Pancreatic islet transplantation
... that islet transplantation significantly reduces fear of hypoglycemia but have little impact on health-related overall QoL [20], others identified a signif- icant improvement with the use of a diabetes-specific ...
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Islet alloautotransplantation: Allogeneic pancreas transplantation followed by transplant pancreatectomy and islet transplantation
... successful islet transplantation with islets isolated from a pancreas allograft that had been removed in an emergency setting due to life-threatening bleeding from a mycotic aneurysm of the ...Subsequent ...
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Improving the use of donor organs in pancreas and islet of Langerhans transplantation
... Sufficient islet numbers can be obtained from a single donor, but generally more than one islet preparation per recipient is required to obtain insulin independence (1, 3, ...the islet ...
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Human mesenchymal stem cells improve rat islet functionality under cytokine stress with combined upregulation of heme oxygenase-1 and ferritin
... Rat islet isolation was performed in accordance with the European Council Directive 2010/63/UE and the recom- mendations of the French Ministry of Agriculture (2013-118) on the care and use of laboratory ...
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Mesenchymal stem cell therapy in type 2 diabetes mellitus
... by key transcription factors such as Pdx-1, Ngn-3, Neu- roD1, Pax4, and Pax6 [36]. Correct reprogramming of cells to activate these pathways is necessary for induc- ing differentiation of MSCs into IPCs. Chen et al. ...
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Association of insulin, C-peptide and blood lipid patterns in patients with impaired glucose regulation
... the islet β cells produce an insulin molecule and measurements of insulin and C-peptide can be used to reflect the reserve functional status of islet β ...
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Is Islet Transplantation a Realistic Therapy for the Treatment of Type 1 Diabetes in the Near Future?
... This widespread implementation of the Edmonton protocol is already driv- ing the standardization of islet process- ing techniques and will further reveal the toxicity (or lack thereof) of the immunosuppressive ...
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Pancreatic β- and α-cell adaptation in response to metabolic changes
... mg/kg BrdU subcutaneously twice daily during the final 7 days of the 6-week study period. The transplanted recipient mice received 1 mg/ml BrdU in the drinking water (refreshed every other day) during the final 7 days. ...
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Endothelial chimerism and vascular sequestration protect pancreatic islet grafts from antibody mediated rejection
... showing islet graft outer limit (white dotted line) and ROI localization (white dashed circles), which were positioned outside islet graft vasculature (white ...on islet graft 5 minutes after ...
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Vascular endothelial growth factor coordinates islet innervation via vascular scaffolding
... during islet development and in mature islets, we showed that: (1) establishment of the intra-islet vasculature by VEGF is essential for the postnatal maturation of islet innervation; (2) ...
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Islet stress, degradation and autoimmunity
... to increased alternative mRNA splicing events providing evidence for β -cell neo-autoantigens generation during insulitis. 4 The inflammatory milieu also impacts post-translational modification (PTM) processes: the ...
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Heat shock induces resistance in rat pancreatic islet cells against nitric oxide, oxygen radicals and streptozotocin toxicity in vitro
... induced islet cell death, was suppressed after heat shock (P < ...pancreatic islet cells can rapidly activate defence mechanisms against nitric oxide, reactive oxygen intermediates and streptozotocin by ...
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