isolated hepatocytes
Inhibition of glutathione efflux in the perfused rat liver and isolated hepatocytes by organic anions and bilirubin Kinetics, sidedness, and molecular forms
... Using isolated, in situ, single-pass perfused rat livers, incubations of freshly isolated hepatocytes, and sinusoidal membrane-enriched vesicles, we and others have shown the saturability of ...
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Regulation of heat shock genes in isolated hepatocytes from an Antarctic fish, Trematomus bernacchii
... in isolated hepatocytes ...treated hepatocytes were equivalent to levels in field-caught specimens sacrificed immediately upon capture, precluding handling stress as the cause of the observed Hsp70 ...
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Studies on the mechanism of insulin resistance in the liver from humans with noninsulin dependent diabetes Insulin action and binding in isolated hepatocytes, insulin receptor structure, and kinase activity
... human hepatocytes from an intraoperative liver biopsy to study insulin action and resistance in ...man. Hepatocytes from obese patients with noninsulin-dependent diabetes were resistant to maximal insulin ...
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Glucocorticoid induced Insulin Resistance: THE IMPORTANCE OF POSTBINDING EVENTS IN THE REGULATION OF INSULIN BINDING, ACTION, AND DEGRADATION IN FRESHLY ISOLATED AND PRIMARY CULTURES OF RAT HEPATOCYTES
... hypothesis we have studied insulin action, binding, and degradation in freshly isolated hepatocytes from rats rendered insulin resistant by the administration of dexamethasone, 1.0 mg/kg every other day, ...
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In Vitro and In Vivo Hepatoprotective Effect of Vitex Negundo Leaves
... were isolated by a modified procedure of Seglen (1979) [7] ...release hepatocytes into the ...These isolated hepatocytes were cultured in Ham’s F12 medium, supplemented with 10% newborn calf ...
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BIO-ARTIFICIAL LIVER
... the isolated hepatocytes are suspended in solution but they can be used in variety of configuration such as substrate attached an encapsulated in semi- permeable ...of hepatocytes based on their use ...
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Alcohol consumption impairs hepatic protein trafficking: mechanisms and consequences
... Many proteins can be modified by acetaldehyde, including tubulin, actin, calmodulin, hemoglobin, hepatic enzymes and plasma proteins [31, 50, 51, 57, 84, 112]. In general, acetaldehyde is thought to form stable adducts ...
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Heat shock protein expression is absent in the antarctic fish Trematomus bernacchii (family Nototheniidae)
... To determine whether a heat-shock response has been retained in Antarctic notothenioid fishes during their 14–25 million years of evolution in a cold and thermally stable environment, we employed well-established methods ...
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Pyruvate dehydrogenase complex stimulation promotes immunometabolic homeostasis and sepsis survival
... DCA physiologically closes a metabolic gap between the low catabolic energy supply associated with immune tolerance and dysregulated organ recovery and the high energy-consuming anabolic processes required by effector ...
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Metabolism of androstenone, beta estradiol and dihydrotestosterone in primary cultured pig hepatocytes and the role of 3 beta hydroxysteroid dehydrogenase in this process
... production [14]. Moreover, an excessive accumulation of androstenone in pig adipose tissue contributes to an unpleasant odour of meat from some entire male pigs which makes the meat unsuitable for human consumption [5]. ...
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In Vitro Hepatoprotective Activity of Justicia Gendarussa Stem on Isolated Rat Hepatocytes
... of hepatocytes resulting in the leakage of cellular ...of isolated hepatocytes with extract of JG moderately restored their viability as well as altered biochemical parameters induced by hepatotoxin ...
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Cathepsin B contributes to TNF α–mediated hepatocyte apoptosis by promoting mitochondrial release of cytochrome c
... mouse hepatocytes to the cytokine in vitro and in vivo. Isolated hepatocytes treated with TNF-α in the presence of the transcription inhibitor acti- nomycin D (AcD) accumulated cat B in their ...of ...
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A novel role for ursodeoxycholic acid in inhibiting apoptosis by modulating mitochondrial membrane perturbation
... in isolated hepatocytes and human HuH-7 hepatoma ...rat hepatocytes at concentrations similar to those found during cholestasis (4, ...in hepatocytes (36, ...
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Primitive organization of cytosolic Ca(2+) signals in hepatocytes from the little skate Raja erinacea
... and hepatocytes were manually dispersed with forceps. The isolated hepatocytes were passed twice through a 60 µmol l −1 nylon mesh filter, subjected to centrifugation twice at 100 g and the pellet ...
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Na+/H+ exchange and osmotic shrinkage in isolated trout hepatocytes
... Also, in any environment, fish behaviour and metabolism may produce alterations in acid–base balance. For example, during exercise stress, in vivo studies have shown that the extracellular pH, but not pHi, was greatly ...
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Hypoxic preconditioning potentiates the trophic effects of mesenchymal stem cells on co-cultured human primary hepatocytes
... Intracellular ROS are known to modulate the bio- logical activities of MSCs, including cellular prolifera- tion, survival/apoptosis, and attachment. Exposure to moderate hypoxia is known to immediately increase intra- ...
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Metabolism of reverse triiodothyronine by isolated rat hepatocytes
... by isolated rat hepatocytes was analyzed by Sephadex LH-20 chromatography, high performance liquid chromatography, and radioimmunoassay, with closely agreeing ...rat hepatocytes is only observed if ...
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Preliminary safety evaluation of novel small molecule inhibitors of caspase 3
... freshly isolated rat primary hepatocytes was up-to 30µM for all the tested ...the hepatocytes toxicity when cells were treated with flavopiridol (flavonoid as pan-CDK ...
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Apolipoproteins E and AV mediate lipoprotein clearance by hepatic proteoglycans
... TRLs in the circulation undergo lipolytic processing primarily by LPL immobilized on the capillary endothelial surface by way of its receptor GPIHBP1, resulting in triglyceride hydrolysis and release of free fatty acids ...
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Uptake and Metabolism of L Alanine by Freshly Isolated Trout (Salmo Trutta) Hepatocytes: The Effect of Fasting
... Because there is a positive relationship between L-alanine uptake and glucose production in these cells, which is independent of the duration of fasting, a rise in the glucagon-like-pept[r] ...
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