ISOLATED RAT HEPATOCYTES
Metabolism of reverse triiodothyronine by isolated rat hepatocytes
... by isolated rat hepatocytes was analyzed by Sephadex LH-20 chromatography, high performance liquid chromatography, and radioimmunoassay, with closely agreeing ...by rat hepatocytes is ...
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Lysosomal association of internalized 125I insulin in isolated rat hepatocytes Direct demonstration by quantitative electron microscopic autoradiography
... and isolated rat hepatocytes have demonstrated that labeled insulin initially localizes to the plasma membrane and is subsequently internalized to a limited region of the peripheral ...with ...
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In Vitro Hepatoprotective Activity of Justicia Gendarussa Stem on Isolated Rat Hepatocytes
... adult rat was used for the study. Hepatocytes were isolated using modified method of Kiso et al (9) ...The isolated rat hepatocytes were cultures in DMEM at density of 1X 10 6 ...
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Binding, internalization, and lysosomal association of 125I glucagon in isolated rat hepatocytes A quantitative electron microscope autoradiographic study
... freshly isolated rat hepatocytes and studied by quantitative electron microscope autoradiography, the labeled material localizes to the plasma membrane of the cell at early times of incubation of 20 ...
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SUPPRESSION OF VLDL-TRIACYLGLYCEROL SECRETION B Y BOTH α AND β-ADRENOCEPTOR AGONISTS IN ISOLATED RAT HEPATOCYTES
... The effects of alpha and beta-adrenergic stimulation on triacylglycerol secre tion were investigated in isolated rat hepatocytes. Epinephrine within 3h of incu bation suppressed triacylglycerol ...
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Protective Effects of Saponin Mixture, Isolated from Astragalus monspessulanus subsp monspessulanus on Tert Butyl Hydroperoxide—Induced Oxidative Stress in Isolated Rat Hepatocytes
... Even though there is a wide range of drugs that are currently employed in the management of hepatic disord- ers, alternative approach based on the use of traditional herbal preparations and plant isolated ...
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Regulation of ethanol metabolism in isolated rat hepatocytes : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy at Massey University
... The system was primed with 150 ml of enzyme-free perfusion medium. En route the medium was oxygenated by continual gassing with Ch + C(h (95: 5) and maintained at a constant temperature of 37°C and pH of 7.4 (see section ...
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Conjugated linoleic acid isomers modulate protein expression profile in rat hepatocytes
... freshly isolated rat hepatocytes incubated in the presence of different CLA ...in hepatocytes was demonstrated by the altered expression pattern of several proteins involved in lipid ...
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Induction of heme oxygenase-1 and heat shock protein 70 in rat hepatocytes: The role of calcium signaling
... Abstract: Stress response genes including heat shock proteins are induced under a variety of conditions to confer cellular protection. This study investigated the role of calcium signaling in the induction of two stress ...
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Does Ethanol Play a Pro Oxidant Role during Oxidative Stress in the Liver?
... both hepatocytes and FTO2B ...when hepatocytes were treated at the same time with Ethanol and ...of isolated rat hepatocytes to allyl alcohol, diethyl maleate and bromoisovalerylurea ...
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Activin A: an autocrine inhibitor of initiation of DNA synthesis in rat hepatocytes
... of hepatocytes in a similar manner, either activin A or TGF-beta did not compete with each other in their binding when assessed by competitive binding using an iodinated ...When hepatocytes were incubated ...
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Effects of hyposmotic shock on ion fluxes in isolated trout hepatocytes
... Time course analysis of changes affecting the cellular ionic content indicates that after 20 min incubation in the presence of lmmoll" 1 ouabain, potassium fell to 80 ± 2 % N= 13 of its [r] ...
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Na+/H+ exchange and osmotic shrinkage in isolated trout hepatocytes
... the presence of amiloride (10 −3 mol l −1 ) in isotonic H-MEM ( h ) or hypertonic conditions ( d ). The uptake of isotope during a labelling period of 1 min is expressed as percentage of the mean uptake by ...
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Reversible inhibition of urea exchange in rat hepatocytes
... indicator), and [14C]urea were administered into the portal vein and fluid was collected from the hepatic vein. Under control conditions, [14C]urea and 3HOH emerged from the hepatic vein at nearly the same rate. However ...
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Rat hepatocytes exhibit basolateral Na+/HCO3 cotransport
... approximately 10-fold by 25 mM extracellular HCO3-.HCO3--stimulated Na+ uptake was saturable, abolished by 4-acetamido-4'-isothiocyano-2,2'-disulfonic acid stilbene (SITS), and unaffected by amiloride or Cl- removal. ...
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Role of insulin in lipoprotein secretion by cultured rat hepatocytes
... Control experiments, in which exogenous 125I-VLDL or endogenously labeled [14C]VLDL were added to cultures, revealed that the insulin-induced differences in VLDL accumulation and the lip[r] ...
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No evidence for protective erythropoietin alpha signalling in rat hepatocytes
... cultured hepatocytes and per- fused liver, reverse transcription into cDNA and real-time PCR were performed as described ...of rat EPO receptor sequence ...Cultured rat hepatocytes and UT-7 ...
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Transformation of differentiated rat hepatocytes with adenovirus and adenovirus DNA.
... To define more specifically the adenovirus genes required for transforming nonreplicating differentiated hepatocytes, the cells were transfected with plasmids containing only the adenovi[r] ...
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Protein synthesis in hepatocytes isolated from patients with gastrointestinal malignancy
... by hepatocytes (HPS) isolated from 31 patients undergoing liver wedge biopsy: 7 patients with benign disease, 14 with gastric cancer, and 10 with colorectal cancer (5 of whom had liver ...
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Insulin like growth factor I binding in hepatocytes from human liver, human hepatoma, and normal, regenerating, and fetal rat liver
... human hepatocytes, compared with HEP-G2 cells, have virtually no IGF-I binding ...adult rat hepatocytes have low IGF-I binding sites similar to those in human liver, hepatocytes from ...
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