Jurkat Cells
Effects of canola and corn oil mimetic on Jurkat cells
... On Jurkat cells 130 μM linoleic acid or 60 μM a-linolenic acid had a prooxidant- induced antiproliferatve effect that was negatively corre- lated with caspase 3 activation ...of cells in S phase ...
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Original Article Knockdown of WISP1 inhibit proliferation and induce apoptosis in ALL Jurkat cells
... K562, Jurkat, Raj and Molt4 were used to detect the mRNA and protein expression level (Figure 1A, ...that Jurkat cells had the highest WISP1 expression level than that of other cell ...selected ...
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Engineered Jurkat Cells for Targeting Prostate-Specific Membrane Antigen on Prostate Cancer Cells by Nanobody-Based Chimeric Antigen Receptor
... LNCaP cells compared to DU-145 cells. The use of mock-transfected cells revealed no difference between positive and negative ...T cells faced the target cells, T cells were ...
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Stimulation of lymphokines in Jurkat cells persistently infected with vaccinia virus.
... Results from plaque assays and fluorescence-activated cell sorter FACS analyses of virus antigens demonstrated that a persistent infection in which the percentage of infected cells and t[r] ...
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Original Article Up-regulation of VEGF and its receptor in refractory leukemia cells
... Akt (t-Akt) expression and its phosphorylation (p-AKT) status in leukocytes of the participants were analyzed with western blots. Results: Healthy children and the remission group had the lowest blood plasma VEGF ...
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In vitro Effects of Propranolol on T Helper Type 1 Cytokine Profile in Human
... In the present study we showed that propranolol inhibited the PHA-induced secretion of IL-2 and IFN- γ by human leukemic T (Molt-4 and Jurkat) cells. Decrease of IFN-γ by propranolol in our findings is ...
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CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis via the Fas-FasL-mediated pathway
... CpG-ODN-treated Jurkat cells using Trizol reagent, according to the manufacturer ’ s instructions (Invitrogen, Carlsbad, CA, USA), and reversely transcribed into cDNA using oligo (dT) 12-18 and ReverTraAce- ...
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Cell-Type-Specific Tyrosine Phosphorylation of the Herpes Simplex Virus Tegument Protein VP11/12 Encoded by Gene UL46
... T cells, Lck plays a key role in signaling through the TCR, where it phosphorylates the CD3 and TCR chains of the TCR com- plex in response to TCR ligation (reviewed in reference ...in Jurkat T ...
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Antileukemic activity of Houttuynia cordata Thunb extracts in Jurkat and U937 human leukemic cells
... of Jurkat and U937 cells were determined by ELISA and illustrated in Figure ...in Jurkat and U937 ...of Jurkat cells was regulated by NF-κB and AP-1 from the MAPK pathway [20-21] and by ...
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Inhibition of autophagy potentiates anticancer property of 20(S)-ginsenoside Rh2 by promoting mitochondria-dependent apoptosis in human acute lymphoblastic leukaemia cells
... mammalian cells via inhibition of the class III PI3K activity ...and Jurkat cells, the number of GFP- LC3 puncta per cell was significantly increased in cells treated with 3-MA and 20(S)-GRh2, ...
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Membrane fatty acid heterogeneity of leukocyte classes is altered during in vitro cultivation but can be restored with ad-hoc lipid supplementation
... The present study shows that the various classes of leu- kocytes have a specific membrane fatty acid profile in vivo that is unlike that of other leukocytes, and which makes the membrane properties of different cell ...
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Destabilisation of dimeric 14-3-3 proteins as a novel approach to anti-cancer therapeutics
... 14-3-3 proteins play a pivotal role in controlling cell proliferation and survival, two commonly dysregulated hallmarks of cancers. 14-3-3 protein expression is enhanced in many human cancers and correlates with more ...
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EXPLORATION INTO THE CELLULAR EFFECTS OF AZIRIDINOMITOSENES
... in Jurkat and HeLa cells also provides evidence that Jurkat cell DNA is more vulnerable to DNA ...treated Jurkat cells compared to similar treatment of HeLa ...
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Rhamnazin inhibits proliferation and induces apoptosis of human jurkat leukemia cells in vitro
... mediated apoptosis. Caspase8 may be involved in apoptosis induced by various cytotoxic agents, in cluding etoposide [16], although the significance of this phenomenon for activation of apoptotic effector pathways has ...
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Strong trans activation of the human cytomegalovirus major immediate-early enhancer by p40tax of human T-cell leukemia virus type I via two repetitive tax-responsive sequence elements.
... Lanes 1, 2, and 7 to 16, Jurkat cells were transfected with plasmid pRR55, which contains the HCMV enhancer/promoter upstream of the CAT gene; lanes 3 and 4, Jurkat cells were transfecte[r] ...
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Tat- and Rev-directed antisense RNA expression inhibits and abolishes replication of human immunodeficiency virus type 1: a temporal analysis.
... Expression of CD4 surface antigen and HIV-1 replication in parental Jurkat cells and Jurkat clones stably transfected with the antisense RNA expression plasmid pAR6 or the control plasmi[r] ...
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The role of aberrant expression of T cell miRNAs affected by TNF α in the immunopathogenesis of rheumatoid arthritis
... in Jurkat cells affected the expression of 13 miR- NAs, and nine miRNAs were found to be downregulated in T cells from RA ...T cells, short-term TNF-α exposure is important for inflamma- tory ...
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Combinatorial Latency Reactivation for HIV-1 Subtypes and Variants
... infections. Jurkat cells were infected at a low MOI with LGIT (1a) or LG (1b) ...virus-infected cells (2a) or Tat protein, HMBA, and TNF- ␣ for LG virus-infected cells ...) cells were ...
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Platelet-derived sphingosine 1-phosphate induces migration of Jurkat T cells
... 5 Jurkat cell migration induced by the supernatant from activated platelets ...(A) Jurkat cells were seeded into the upper chamber of the Transwell cell culture chambers, and allowed to migrate for 4 ...
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A critical role for the TAR element in promoting efficient human immunodeficiency virus type 1 reverse transcription.
... either Jurkat cells or ...Ag. Jurkat cells ...either Jurkat cells or PBMCs ...both Jurkat cells and PBMCs, exhibiting defects in replication ranging from 100- to ...
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