kinesin-1
Dual control of Kinesin 1 recruitment to microtubules by Ensconsin in Drosophila neuroblasts and oocytes
... Ensconsin Kinesin-binding domain can promote recruitment of K560 to MTs in vitro ...acids 1-560), were mixed in the presence of polymerized MTs and subjected to centrifugation (see Materials and ...dual ...
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Inefficient recruitment of kinesin 1 to melanosomes precludes it from facilitating their transport
... abundant kinesin-1 heavy chain in melanocytes) did not affect the dispersed distribution of melanosomes in wild-type melanocytes or the ability of GFP-myosin-Va to rescue melanosome clustering in myosin-Va ...
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Drosophila PAT1 is required for Kinesin 1 to transport cargo and to maximize its motility
... Kinesin-1 is composed of two heavy chains (KHC) and two light chains (KLC), which are encoded by single genes in Drosophila melanogaster. KHC has an N-terminal motor domain, a dimeric coiled-coil domain ...
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Nesprins anchor kinesin 1 motors to the nucleus to drive nuclear distribution in muscle cells
... protein kinesin-1. Loss of kinesin-1 leads to improperly positioned nuclei in culture and in ...how kinesin-1 functions to move nuclei in ...model, kinesin-1 acts ...
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Kinesin 1 tail autoregulation and microtubule binding regions function in saltatory transport but not ooplasmic streaming
... for Kinesin-1 function: the stalk of a cargo- free motor folds such that the IAK region interacts with and inhibits force generation by the head, then cargo binding via Klcs or analogous linkers activates ...
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Kinesin 1 interacts with Bucky ball to form germ cells and is required to pattern the zebrafish body axis
... In animals, specification of the primordial germ cells (PGCs), the stem cells of the germ line, is required to transmit genetic information from one generation to the next. Bucky ball (Buc) is essential for germ plasm ...
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Delineation of the TRAK binding regions of the kinesin 1 motor proteins
... cells. Kinesin-1, the first kinesin to be identified, is a heterotetramer consisting of two heavy chains and two light ...with kinesin via the cargo binding domain or via the light chains, ...
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Kinesin-1 Acts with Netrin and DCC to Maintain Sensory Neuron Position in Caenorhabditis elegans
... ABSTRACT The organization of neurons and the maintenance of that arrangement are critical to brain function. Failure of these processes in humans can lead to severe birth defects, mental retardation, and epilepsy. ...
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UNC 83 is a nuclear specific cargo adaptor for kinesin 1 mediated nuclear migration
... A KLC-2::KASH chimeric protein partially bypasses the requirement of UNC-83 for nuclear migration The data from the above experiments suggest a model in which UNC-83 on the outer nuclear envelope functions as an adapter ...
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The Basic Domain of Herpes Simplex Virus 1 pUS9 Recruits Kinesin-1 To Facilitate Egress from Neurons
... as kinesin-1, as well as vesicular traf- ficking proteins or proteins involved in exocytosis which are en- riched in growth cones ...and kinesin-1, colocalizing with HSV-1 virions in ...
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Direct Interaction of Baculovirus Capsid Proteins VP39 and EXON0 with Kinesin-1 in Insect Cells Determined by Fluorescence Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy
... The interaction of both VP39 and EXON0 with KLC-TPR raises several further research questions, including why both cap- sid proteins interact with KLC-TPR and whether EXON0 and VP39 interact with KLC-TPR consequentially ...
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Insights into kinesin-1 activation from the crystal structure of KLC2 bound to JIP3
... Each kinesin con- tains a conserved motor domain, with variable flanking regions that regulate the activity of the motor domains and mediate bind- ing to effectors, ...that kinesin motor domains are ...
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Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars
... ~2 kinesin molecules in the case of a 700-nm pillar spacing, whereas it can bind to ~12 kinesin molecules in the case of a 300-nm spacing ...the kinesin-1 – patterned surface ...the ...
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Mechanochemistry of the kinesin 1 ATPase
... the kinesin active ...the kinesin active site to be harnessed to drive a larger scale conformational cycle that in turn can do substantial work, and it also allows external forces sensed by the motor to ...
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HIV-1 Uncoating Is Facilitated by Dynein and Kinesin 1
... type 1 (HIV-1) must reverse transcribe its RNA genome to DNA and traffic to the nuclear envelope, where the viral genome is translocated into the nucleus for subsequent integration into the host cell ...the ...
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Simulating the kinesin walk : towards a definitive theory
... Conventional kinesin (kinesin-1) is a motor protein that performs a vital function in the eukaryotic cell: it actively transports cargo to required ...destinations. Kinesin pulls cargo along ...
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Kinesin light chain-1 serine-460 phosphorylation is altered in Alzheimer’s disease and regulates axonal transport and processing of the amyloid precursor protein
... on kinesin-1 motors and one route for this transport involves calsyntenin-1, a type-1 membrane spanning protein that acts as a direct ligand for kinesin-1 light chains ...
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Mechanical inhibition of microtubule depolymerisation by kinesin
... literature. Kinesin-1 has been shown to fully-decorate subgroups of homogeneous MT populations in vitro , whilst not binding to others in the same sample ...of kinesin-MT binding kinetics are biased ...
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Role of Microtubules and Microtubule-Associated Proteins in HIV-1 Infection
... the kinesin-1 motor KIF5B in HIV-1 uncoating, an observation that is in agreement with a subsequent study by Malikov and Naghavi, who observed that HIV-1 uncoating was dependent on both KIF5B ...
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Structural and biomechanical basis of mitochondrial movement in eukaryotic cells
... superfamily, kinesin-1, was isolated as a heterotetrameric fast axonal organelle transport motor consisting of two iden- tical motor subunits (KFCs; kinesin heavy chains) and two “light chains” ...
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