Kinetoplast DNA
Identification of New Kinetoplast DNA Replication Proteins in Trypanosomatids Based on Predicted S-Phase Expression and Mitochondrial Targeting
... Trypanosomatid parasites contain an unusual form of mitochondrial DNA (kinetoplast DNA [kDNA]) consisting of a catenated network of several thousand minicircles and a smaller number of maxicircles. ...
8
SYBR Green based Real Time PCR targeting kinetoplast DNA can be used to discriminate between the main etiologic agents of Brazilian cutaneous and visceral leishmaniases
... Leishmania kinetoplast DNA minicircles [5’-GGC CCA CTA TAT TAC ACC AAC CCC-3’ and 5’-GGG GTA GGG GCG TTC TGC GAA-3’] ...template DNA and distilled ultra-pure water for a final reaction volume of 15 μ ...
9
Is the kinetoplast DNA a percolating network of linked rings at its critical point?
... the kinetoplast genome, modelled as a large number of semiflexible unknotted loops, which are allowed to link with each ...the DNA density increases, the systems shows a percolation transition between a gas ...
10
Redox Potential Regulates Binding of Universal Minicircle Sequence Binding Protein at the Kinetoplast DNA Replication Origin
... Kinetoplast DNA, the mitochondrial DNA of the trypanosomatid Crithidia fasciculata, is a remarkable structure containing 5,000 topologically linked DNA ...of DNA differ from those ...
12
Three Mitochondrial DNA Polymerases Are Essential for Kinetoplast DNA Replication and Survival of Bloodstream Form Trypanosoma brucei
... mitochondrial DNA network of trypanosomatids called kinetoplast DNA ...This DNA network is housed within the parasite’s single mi- tochondrion and contains topologically interlocked circular ...
10
TbKAP6, a Mitochondrial HMG Box-Containing Protein in Trypanosoma brucei, Is the First Trypanosomatid Kinetoplast-Associated Protein Essential for Kinetoplast DNA Replication and Maintenance
... the kinetoplast in situ by thin-section EM of uninduced cells and TbKAP6-depleted cells (RNAi for 4 days) ...the kinetoplast disk exhibited a characteristic striated, densely stained disk ...The ...
14
Kinetoplast DNA Network: Evolution of an Improbable Structure
... Kinetoplast DNA (kDNA) is the most structurally complex mitochondrial DNA in nature. Unique to the single mitochon- drion of unicellular flagellates of the order Kinetoplastida, kDNA is best known as ...
8
Variability of Kinetoplast DNA Gene Signatures of Trypanosoma cruzi II Strains from Patients with Different Clinical Forms of Chagas' Disease in Brazil
... The clinical course of Chagas’ disease varies widely among different patients and geographic regions. For reasons that are not completely understood but involve host and parasite factors, some patients never develop the ...
5
Stem-Loop Silencing Reveals that a Third Mitochondrial DNA Polymerase, POLID, Is Required for Kinetoplast DNA Replication in Trypanosomes
... Kinetoplast DNA (kDNA), the mitochondrial genome of trypanosomes, is a catenated network containing thousands of minicircles and tens of ...mitochondrial DNA polymerases (Pols) for kDNA ...A ...
6
Cell cycle synchronisation of Trypanosoma brucei by centrifugal counter flow elutriation reveals the timing of nuclear and kinetoplast DNA replication
... A reliable method for cell cycle synchronisation is an invaluable tool to study cell cycle regulation, but the quality of synchronised cells populations produced cannot be easily scored. Cooper has suggested a range of ...
10
Luteolin, an Abundant Dietary Component is a Potent Anti-leishmanial Agent that Acts by Inducing Topoisomerase II-mediated Kinetoplast DNA Cleavage Leading to Apoptosis
... minicircle DNA, the cell lysates of quercetin- and luteolin-treated parasites were extracted with phenol-chloro- form, with and without prior proteinase K ...minicircle DNA molecules were linked covalently ...
15
Trypanosoma brucei Tb927.2.6100 Is an Essential Protein Associated with Kinetoplast DNA
... Tb927.2.6100 itself consisted of only two identified peptides, which may be a consequence of the location and abundance of its lysine and arginine residues that may have affected susceptibility to protease and the ...
9
A Nested PCR Based Schizodeme Method for Identifying Leishmania Kinetoplast Minicircle Classes Directly from Clinical Samples and Its Application to the Study of the Epidemiology of Leishmania tropica in Pakistan
... the kinetoplast minicircles of all Leishmania species which infect ...10,000 kinetoplast DNA minicircles, which are unequally distributed among approximately 10 minicircle ...month. DNA for ...
5
Cell Cycle-Dependent Localization and Properties of a Second Mitochondrial DNA Ligase in Crithidia fasciculata
... second kinetoplast DNA ligase gene in ...a kinetoplast DNA ligase (LIG k  ) in ...mitochondrial DNA ligase gene in the ...mitochondrial DNA ligase gene (LIG k ␣ ) upstream of ...
8
Mitochondrial Histone-Like DNA-Binding Proteins Are Essential for Normal Cell Growth and Mitochondrial Function in Crithidia fasciculata
... chondrial DNA, termed kinetoplast DNA (kDNA), is one of the most complex and unusual DNAs in nature (10, 16, ...the kinetoplast and consists of two types of circular DNA molecules, ...
9
Depletion of Mitochondrial Acyl Carrier Protein in Bloodstream-Form Trypanosoma brucei Causes a Kinetoplast Segregation Defect
... Like other eukaryotes, trypanosomes have an essential type II fatty acid synthase in their mitochondrion. We have investigated the function of this synthase in bloodstream-form parasites by studying the effect of a ...
7
Detailed interrogation of trypanosome cell biology via differential organelle staining and automated image analysis
... our DNA staining and image analysis tools and adapt them for related ...of kinetoplast and nucleus location could be used to measure the location of any fluorescently labeled structure in the ...two ...
18
Comparison of Leishmania OligoC TesT PCR with Conventional and Real Time PCR for Diagnosis of Canine Leishmania Infection
... mania DNA sequences and experimental PCR protocols used in laboratories worldwide, which complicates objective comparisons of test sensitivity and ...parasite DNA in hu- man specimens (3, ...of ...
6
A Second Mitochondrial DNA Primase Is Essential for Cell Growth and Kinetoplast Minicircle DNA Replication in Trypanosoma brucei
... mitochondrial DNA of trypanosomes contains two types of circular DNAs, minicircles and ...mitochondrial DNA primases, PRI1 and PRI2, related to the primases of eukaryotic nucleocytoplasmic large DNA ...
10
PNT1 is a C11 Cysteine Peptidase Essential for Replication of the Trypanosome Kinetoplast.
... the kinetoplast, a unique organelle containing the organism’s mitochondrial ...The kinetoplast DNA (kDNA) of ...mitochondrial DNA is coordinated with cytokinesis (18) and the presence of ...
18