Knock-In Mouse
The distinct biological implications of Asxl1 mutation and its roles in leukemogenesis revealed by a knock-in mouse model
... wild-type mouse marrow precursor cells, with or without MN1 overexpression, highlighted the association of in vivo Asxl1 mutation to the expression of hypoxia, multipotent progenitors, hematopoietic stem cells, ...
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Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line
... Pax7-YFP knock-in mice by FACS without anti- body staining, similar to cells isolated from transgenic Pax7-ZsGreen, Pax7-nGFP , and Pax7-GFP reporter mouse lines that have recently been reported ...Pax7-YFP ...
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Modeling rare gene variation to gain insight into the oldest biomarker in autism: construction of the serotonin transporter Gly56Ala knock-in mouse
... a knock-in mouse will allow us to answer a number of questions, ranging from basic to ...this knock-in allele will parallel native mouse ...the mouse, initially using synaptosome ...
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Vascular and parenchymal amyloid pathology in an Alzheimer disease knock-in mouse model: interplay with cerebral blood flow
... transgenic mouse models expressing mutant APP with or without mutant PSEN1 under the control of exogenous promoters have been created to model Aβ ...The knock-in mouse models are particularly ...
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Comprehensive behavioral characterization of an APP/PS-1 double knock-in mouse model of Alzheimer's disease
... KI mouse model, less is known about AD-relevant behavioral/cognitive deficits of this ...KI mouse using a cross-sectional design (age groups of 7, 11, 15, and 24 months ...KI mouse shows no motor ...
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An impaired intrinsic microglial clock system induces neuroinflammatory alterations in the early stage of amyloid precursor protein knock-in mouse brain
... circadian rhythms is linked to inflammatory pathologies. Previous studies have noted the importance of the circadian clock in age-related neuroinflammatory sensitization [27, 28]. BMAL1 is the central mediator of the ...
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Reversible External Control of Gene Expression in an NR1 Knock-in Mouse
... knockout mouse is viable) even when expression from the targeted NRl is completely ...homozygous mouse is mated with a heterozygous mouse, it is expected that 50% of their offspring jg ...
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Leaky Ca2+ release channel/ryanodine receptor 2 causes seizures and sudden cardiac death in mice
... a knock-in mouse model of a RYR2 missense mutation (R2474S) found in CPVT patients (22) and provide evidence supporting a causal role for leaky RyR2 channels in both epilepsy and fatal ventricular ...
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Distinct functional consequences of ECEL1/DINE missense mutations in the pathogenesis of congenital contracture disorders
... another knock-in mouse with a distinct pathogenic mutation is necessi- tated to judge whether axonal arborization defects are a common mechanism in the pathogenesis of ECEL1-mu- tated ...DINE ...
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Radio-deoxynucleoside Analogs used for Imaging tk Expression in a Transgenic Mouse Model of Induced Hepatocellular Carcinoma
... The mouse model with dual reporters: HSV1-tk and luc placed under the transcrip- tional control of an endogenous Afp promoter was used for imaging ...created knock-in mouse model was used to ...
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Genetic and pharmacological inhibition of calcineurin corrects the BDNF transport defect in Huntington's disease
... human- mouse hybrids derived from mouse huntingtin cDNA: first exon of mouse huntingtin has been substituted by the homologous human one in mouse full length cDNA ...targeting mouse ...
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Characterising Mutational Spectra of Carcinogens in the Tumour Suppressor Gene TP53 Using Human TP53 Knock-in (Hupki) Mouse Embryo Fibroblasts
... Abstract: DNA in dividing cells is prone to mutagenesis, with mutations making key contributions to human disease including cancer. The tumour suppressor gene TP53 is the most frequently mutated gene in human tumours. ...
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Knock your SOCS off!
... compared with that in controls. While these observations appear clear-cut, para- doxically, marked overexpression of SOCS2 in a transgenic mouse also led to growth excess (14). Thus SOCS2 in fact acts as a dual ...
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Dysregulated brain creatine kinase is associated with hearing impairment in mouse models of Huntington disease
... Huntington disease (HD) is a degenerative disorder caused by expanded CAG repeats in exon 1 of the hunting- tin gene (HTT). Patients with late-stage HD are known to have abnormal auditory processing, but the periph- eral ...
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Alterations in the self renewal and differentiation ability of bone marrow mesenchymal stem cells in a mouse model of rheumatoid arthritis
... type mouse at 10 weeks of age showing no evidence of synovitis or bone erosion; (b) A representation of a non-inflamed ankle joint in Balb/c IL1ra-/- mouse at 10 weeks of age showing no evidence of ...
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Secreted APP regulates the function of full-length APP in neurite outgrowth through interaction with integrin beta1
... APP knock-out neurons are plated and examined for effects on neurite outgrowth as above, the cultures lack both full-length APP and secreted ...not knock it down (APP- inactive) ...
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In vivo multiplex quantitative analysis of 3 forms of alpha melanocyte stimulating hormone in pituitary of prolyl endopeptidase deficient mice
... the mouse model (PREP-GT), used in this study has shown a mater- nal influence of PREP on the fat mass of adult ...This mouse model has been phenotypically characterized and it exhibits a parent of origin ...
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Protein phosphatase 2A modulates podocyte maturation and glomerular functional integrity in mice
... For label-free, relative, quantitative analysis, we collected podocytes from the control and podocyte-specific PP2A- knockout mice at 4 weeks of age, and then they were then analyzed by the Ptm-biolab in Hangzhou to ...
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The Inhibition and Treatment of Breast Cancer with Poly (ADP-ribose) Polymerase (PARP-1) Inhibitors
... non-cancerous mouse BRCA1- or BRCA2-deficient embryonic stem (ES) cells, and hamster BRCA2-deficient cells have demonstrated that the targeted inhibition of poly(ADP-ribose) polymerase (PARP-1) kills BRCA mutant ...
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GENE KICKED MOUSE: KNOCK OUT MOUSE AND ITS APPLICATION
... particularly informative. Scientists use observations, hypotheses and deductions to propose explanations, theories, for natural phenomena. Predictions from theories are tested by experiment. Any theories which are cogent ...
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