KRAS mutation
KRAS mutation analysis by PCR : a comparison of two methods
... This study evaluates two PCR based assays for KRAS mutation detection in formalin-fixed par- affin-embedded CRC tissue samples. We observed three discrepancies between two mutation detection methods, ...
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Correction: Key differences between 13 KRAS mutation detection technologies and their relevance for clinical practice
... established KRAS mutation detection platforms and ...for KRAS mutation detec- tion, particularly in those derived from small biopsies, which commonly have limited tissue available for ...
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Detection of kras mutation in circulating free dna (cfdna)
... that KRAS analysis in plasma is a viable alternative to tissue ...metastasis KRAS are strongly correlated and hold promise of clinical application (Spindler et ...of KRAS mutation in ...
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Key differences between 13 KRAS mutation detection technologies and their relevance for clinical practice
... (therascreen KRAS RGQ PCR Kit, Qiagen; cobas KRAS Mutation Test, Roche Diagnostics; Idylla, Biocartis, Mechelen, Belgium); matrix-assisted laser desorption/ionisation time-of-flight mass spec- ...
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KRAS Mutation is a Local Tumour Event and Not a Field Change in Pancreatobiliary Tumours
... The KRAS oncogene encodes a protein responsible for signaling in the mitogen activated (MAP)-kinase pathway of intracellular signal ...transcription. KRAS mutation results in continual activation of ...
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A Study on Molecular Expression of KRAS Mutation in Colorectal Carcinoma and Its Correlation with Clinicopathological Findings in a Tertiary Care Hospital
... of KRAS testing like Sanger sequencing with sensitivity of 20 - 30%,Pyrosequencing with 5% and non –sequencing methods like Real time PCR with HRMA having 5% sensitivity, RFLP with sequencing having ...since ...
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Reliability of KRAS mutation testing in metastatic colorectal cancer patients across five laboratories
... (Henry Ford Health System), and one academic research laboratory (Molecular and Medical Genetics, Oregon Health and Science University) were contracted to analyze KRAS mutation status for comparison with ...
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KRAS mutation in secondary malignant histiocytosis arising from low grade follicular lymphoma
... [47–49]. KRAS is one integral component of the RAS-RAF-MEK-ERK signaling pathway that acts upstream of BRAF ...of KRAS mutation is now recommended in colorectal carcinoma, since its presence ...
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Original Article A novel asymmetric and competitive allele specific real-time fluorescent PCR with MGB universal probes for detection of SNPs and point mutations
... identify KRAS G12D mutation with high specificity, without interference by other KRAS mutations at the same ...and KRAS G12D mutation. The lowest detectable percentage of the ...
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Multipoint Kras oncogene mutations potentially indicate mucinous carcinoma on the entire spectrum of mucinous ovarian neoplasms
... Kras mutation is a common phenomenon in many human ...the Kras mutational status along the histological continuum from normal ovaries to the development of benign, borderline and malignant ovarian ...
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Promoter Methylation of the CADM1 and 4 1B Genes Occurs Independently of the EGFR or the KRAS2 Mutation in Non Small Cell Lung Cancer
... the KRAS gene is another common alteration of driver oncogenes in various tumors, including NSCLC and HNSCC [5] [20] ...mutated KRAS knocked-in gene was acti- vated in the airway epithelial cells to develop ...
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<p>Clinical genetic features and related survival implications in patients with surgically resected large-cell lung cancer</p>
... EGFR mutation (two L858R and one E746-A750del), one a BRAF D594N mutation, and there were no cases of ALK, ROS1, or RET rearrangement or MET exon 14-skipping ...
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<p>Poor prognosis of pulmonary sarcomatoid carcinoma with <em>KRAS</em> mutation and <em>ALK</em> fusion</p>
... metastases. KRAS exon 2 mutation and EML4-ALK fusion were identified in carcinoma tissue by IHC and amplification refractory mutation system ...coexistent KRAS mutation and ALK rear- ...
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Molecular testing in oncology: Problems, pitfalls and progress
... BRAF mutation positivity from primary CRC lesions to their associated metastases was confirmed in a separate study ...for KRAS mutation testing leading the authors to suggest that combining DNA from ...
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Unique characteristics of CpG island methylator phenotype (CIMP) in a Chinese population with colorectal cancer
... BRAF/KRAS mutation status) have diagnostic, therapeutic, and prognostic significance in CRC [20–22], their inci- dences, especially those of MSI, BRAF mutation, and CIMP, vary considerably among ...
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Mucinous and Non-Mucinous Adenocarcinoma in Colorectal Cancer Patients
... The mean duration of follow-up was 32 months (range, 12-72 months) and in this interval, there were 26 deaths and 3 patients lost their follow-up and therefore were omitted from survival analysis. All patients had stage ...
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A comparability study of 5 commercial KRAS tests
... 6 KRAS mutation assays in the analysis of primary colorec- tal cancer samples ...lyzed KRAS from tumor sections on glass slides rather than from DNA, and the results were consequently a closer mimic ...
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SCRIB expression is deregulated in human prostate cancer, and its deficiency in mice promotes prostate neoplasia
... Loss of cellular polarity is a hallmark of epithelial cancers, raising the possibility that regulators of polarity have a role in suppressing tumorigenesis. The Scribble complex is one of at least three interacting ...
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Original Article Screening for EGFR and KRAS mutations in non-small cell lung carcinomas using DNA extraction by hydrothermal pressure coupled with PCR-based direct sequencing
... and KRAS mutations correlate with response to tyrosine kinase inhibitors in patients with non-small cell lung carcinoma ...for mutation analysis using PCR-base direct ...of KRAS gene mutations in ...
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Association between clinicopathological features and survival in patients with primary and paired metastatic colorectal cancer and <em>KRAS</em> mutation
... the KRAS mutation rates were ...the KRAS gene mutation, corresponding to a total mutation rate of ...double mutation. The most common mutation site was G12D, which was ...
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