Lactococcus lactis as a production host
Lactococcus lactis M4, a potential host for the expression of heterologous proteins
... certain host functions ...the production of desired recombinant pro- teins carried by some engineered strains, despite their ability to secrete out the proteins to the ...in Lactococcus cells where ...
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Nisin inducible production of listeriolysin O in Lactococcus lactis NZ9000
... L. lactis as an expression host. L. lactis is a GRAS, Gram-positive lactic acid bacterium widely used in the food ...L. lactis has demon- strated significant promise as a means of producing ...
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Production, secretion and purification of a correctly folded staphylococcal antigen in Lactococcus lactis
... WT host strain (Fig- ures 2, 3 and 4a, lane 1 and Figure 4b, lane ...L. lactis can provide stable proteins and preclude the use of an htrA mutant devoid of surface proteolytic activity as the host ...
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A review on Lactococcus lactis: from food to factory
... Lactococcus lactis has progressed a long way since its discovery and initial use in dairy product fermentation, to its present biotechnological applications in genetic engineering for the production ...
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Expanding the recombinant protein quality in Lactococcus lactis
... main host for the production of recombinant proteins of biomedical interest, but conformational stress responses impose severe bottlenecks that impair the production of soluble, proteolytically ...
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Protein secretion in Lactococcus lactis: an efficient way to increase the overall heterologous protein production
... L. lactis is able to secrete proteins ranging from low-(< 10 kDa) to high-(> 160 kDa) molecular mass through a Sec-dependant ...L. lactis are prone to intracellular degradation whereas secretion ...
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D-Tagatose production by Lactococcus lactis NZ9000 Cells Harboring Lactobacillus plantarum L-arabinose Isomerase
... a host for the construction of expression vectors, which was grown in LB medium and supplemented with ampicillin (100 mg/mL) at 37°C with rotary shaking at 200 rpm or on LB medium solidified with ...L. ...
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Getting value from the waste: recombinant production of a sweet protein by Lactococcus lactis grown on cheese whey
... of host systems [35, ...food-grade production system becomes particularly ...L. lactis [44, 45] are comparable in the CW-based medium and in the reference conditions in rich ...
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Lactococcus lactis provides an efficient platform for production of disulfide-rich recombinant proteins from Plasmodium falciparum
... protein production potentially giving very high ...L. lactis are uncharacterized, this system has proved an efficient host for the production of disulfide bonded fragments of Pfs48/45 ...
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Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000
... the host to produce efficiently het- erologous ...L. lactis 9 k-1 with the least decreased genome size presented the low- est effect on protein ...L. lactis 9 k-4 with the largest deletions produced ...
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Production and purification of staphylococcal nuclease in Lactococcus lactis using a new expression-secretion system and a pH-regulated mini-reactor
... useful host to avoid protein degradation [4], whereas in Bacillus subtilis, not all extracellular proteases have been inactivated to date (4 active surface proteases, including the 3 HtrA family members, are ...
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In vitro assessment of hypocholesterolemic activity of Lactococcus lactis subsp. lactis
... Similar results were reported by Liong and Shah (2005b, c) for Bifidobacterium and Lactobacillus strains, respectively. These results emphasize that Lactococcus lactis subsp. lactis is promising in ...
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The Anti-Tumor Effect of Lactococcus lactis
... L. lactis-secreted hsTRAIL in “human system”, however at the same time, not allowing to assess the complex immune interactions between the tumor and its environment, including tumor infiltrating ...L. ...
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Production of Recombinant Peanut Allergen Ara h 2 using Lactococcus lactis
... lational fusion with the signal sequence (Fig. 1). The sig- nal sequence encodes a 32 amino acid signal peptide leading to protein secretion through the sec-dependent pathway [26]. Upon secretion the signal peptide was ...
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Production of Recombinant Protein of Salsola Kali (Sal k1) Pollen Allergen in Lactococcus Lactis
... L. lactis has received extensive attention as a recombinant carrier of oral vaccines for allergy ...L. Lactis has been shown to be higher than those achieved with other LAB such as Lactobacillus ...L. ...
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Lactococcus lactis subsp. lactis as a natural anti-listerial agent in the mushroom industry
... Keywords: Listeria monocytogenes; Lactococcus lactis; mushroom industry; competitive 16 exclusion 17 18 *Corresponding author: 19.. Catherine M..[r] ...
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Identification and characterisation of antimicrobial activity of nisin a produced by Lactococcus lactis subsp lactis LL27
... the production and storage of food and ...ocin production is widespread among lactic acid bacteria, especially in ...Lc. lactis, which are often assumed to be mainly associated with milk and dairy ...
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Optimisation of Fermentative Parameters for GABA Enrichment by Lactococcus lactis
... MAtEriAls And MEthOds Material . L. lactis strain B was isolated from Chinese traditional cabbage kimchi. Brown rice was supplied by Mayang Oil Co. Ltd. in Mayang County, Hunan Province, China. Yoghurt, germi- ...
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Characterization of the Lactococcus lactis lactose genes and regulation of their expression
... Site-directed mutagenesis of the Lactococcus lactis lacR gene, that controls expression of the lacABCDFEGX operon, was carried out to identify amino acid residues in the LacR repressor[r] ...
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LANTIBIOTIC NISIN: NATURAL PRESERVATIVE FROM LACTOCOCCUS LACTIS
... the production of the lantibiotic nisin, lactococcins, lactostrepcins, diplococcins and others 72 ...by Lactococcus lactis subsp. lactis A164 66 ...
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