Laser capture microdissection (LCM)
Laser Capture Microdissection and Isolation of High-Quality RNA from Frozen Endometrial Tissue.
... Laser capture microdissection enables the visualisation and isolation of homogeneous cell populations from tissue sections for subsequent downstream molecular ...for LCM is the fact that ...
18
Implementation and evaluation of amyloidosis subtyping by laser-capture microdissection and tandem mass spectrometry
... of laser capture microdissection (LCM) followed by tandem mass spectrometry (MS) to identify the subtype of amyloid with a high degree of confidence from clinical biopsy samples ...
6
Laser Capture Microdissection Assessment of Virus Compartmentalization in the Central Nervous Systems of Macaques Infected with Neurovirulent Simian Immunodeficiency Virus
... using laser capture microdissection (LCM) to assess whether there was evidence of compartmentalization between these two regions of the ...
13
Laser capture microdissection coupled mass spectrometry (LCM-MS) for spatially resolved analysis of formalin-fixed and stained human lung tissues
... end, laser capture microdissection coupled to mass spectrom- etry (LCM-MS) is a method currently being optimized for microproteomics to determine regional tissue differ- ences ...[3]. ...
12
Ensuring good quality rna for quantitative real time pcr isolated from renal proximal tubular cells using laser capture microdissection
... via laser capture microdissection (LCM), followed by extraction of good quality total RNA sufficient for quantitative real-time polymerase chain reaction (qPCR) ...This LCM-qPCR ...
7
Laser capture microdissection as a tool to evaluate human papillomavirus genotyping and methylation as biomarkers of persistence and progression of anal lesions
... by LCM if possible, are denoted ‘ unas- sessable ’ ...4 Laser capture microdissection of high-grade squamous intraepithelial lesion ...for laser capture ...ultraviolet ...
12
Collection of Epithelial Cells from Rodent Mammary Gland Via Laser Capture Microdissection Yielding High-Quality RNA Suitable for Microarray Analysis
... lysate. Laser capture microdissection (LCM) allows specific cell populations to be harvested, thus reducing the amount of biological noise while increasing the sensitivity of microarrays ...
13
Review Article Laser Capture Microdissection in the Genomic and Proteomic Era: Targeting the Genetic Basis of Cancer
... of microdissection with a primer extension preamplification (PEEP) and whole genome amplification (WGA), it became possible to use smaller and smaller samples of cells, thereby refining the study of preneoplastic ...
14
An improved procedure for isolation of high-quality RNA from nematode-infected Arabidopsis roots through laser capture microdissection
... of LCM on animal and human tissues, it has become increasingly popular among plant scientists because of its ability to isolate a single cell-type from a heterogeneous ...of LCM in plant sciences expands, a ...
9
Distinctions in gastric cancer gene expression signatures derived from laser capture microdissection versushistologic macrodissection
... Microdissection is particularly useful for enriching gastric cancer tumor cells obtained from endoscopic biopsy samples, especially from diffuse-type gastric can- cer which is composed of scattered tumor cells ...
12
Laser capture microdissection (LCM) and whole genome amplification (WGA) of DNA from normal breast tissue optimization for genome wide array analyses
... of LCM, 16 μm sections were cut at -20°C in a cryostat and placed on MembraneSlides (Carl Zeiss ...de/ microdissection) gave a distinct nuclear staining that made it easy to identify breast ducts and ...
7
Proteomic analysis of prolactinoma cells by immuno-laser capture microdissection combined with online two-dimensional nano-scale liquid chromatography/mass spectrometry
... Lysis buffer, containing urea (7 M) and thiourea (2 M), was added to a 0.5 ml Eppendorf tube connected to the LCM cap. Captured cells were solubilized by a series of 20 ms sonication bursts, followed by a 20 s ice ...
11
Specific microRNAs as novel biomarkers for combination chemotherapy resistance detection of colon adenocarcinoma
... by laser cap- ture microdissection (LCM) as previously described ...LMD6000 laser capture microdissection microscope (Leica ...
9
Proteomics: a powerful tool to study plant responses to biotic stress
... tion; LCM: laser capture microdissection; ABPP: activity-based protein profiling; APEX: ascorbate peroxidase; ORMs: organelle-locatable reactive molecules; BioID: proximity-dependent biotin ...
20
Laser capture microdissection of gonads from juvenile zebrafish
... NBT BCIP staining for alkaline phosphatase in gonads Zebrafish were embedded in Tissue-Tech Optimal Cutting Temperature (OCT) compound (Sakura Fintek Europe, Zoeterwonde, NL), rapidly frozen in isopentan on dryice and ...
7
Genomic homogeneity in fibrolamellar carcinomas
... by laser capture microdissection (LCM) (Arctu- rus Engineering ...pulsed laser beam activates a precise spot on the transfer ...
6
Microgenomics in ameloblastoma
... tissue. Laser capture microdissection (LCM) is a technique that allows the isolation of individual cell populations for genetic analysis, thereby circumventing the impact of tissue ...2000). ...
52
Next generation transcriptome sequencing of the premenopausal breast epithelium using specimens from a normal human breast tissue bank
... luteal; LCM: laser capture microdissection; LH: luteinizing hormone; MCM: minichromosome maintenance; MORE: Multiple Outcomes of Raloxifene Evaluation; mRNA: messenger RNA; MT: microtubule; ...
16
BK Virus as a Cofactor in the Etiology of Prostate Cancer in Its Early Stages
... Utilizing laser capture microdissection (LCM) on can- cerous prostates, we further show that the p53 gene from TAg-expressing PIA cells was wild type, whereas tumor cells expressing nuclear ...
10
Transcriptome analysis of the ependymal barrier during murine neurocysticercosis
... using laser capture microdissection from mice infected or mock-infected with the closely related parasite Mesocestoides corti, and analyzed the genes that were differentially expressed using ...
13